Catalytic reduction hemoglobin electrochemical sensor
A hemoglobin, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problem of narrow detection range, and achieve the effects of sensitive detection, simple preparation and low cost
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Embodiment 1
[0017] The electrochemical sensor capable of catalyzing the reduction of hemoglobin of the present invention is a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode as in the prior art, and is characterized in that the working electrode is made according to the following steps:
[0018] a. First, according to the existing technology, use bromide to modify the electrode surface, use the glassy carbon electrode as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum electrode as the counter electrode, and make the working electrode, reference electrode and counter electrode The three-electrode system is put into the pH5.0 sodium acetate-acetic acid buffer solution containing graphene and toluidine blue, and the dosage ratio of the graphene, toluidine blue and pH5.0 sodium acetate-acetic acid buffer solution is 3 μ g: 0.3 mmol: 1L; within the potential range of -0.8 to 1.3V, 25 cycl...
Embodiment 2
[0021] The electrochemical sensor capable of catalyzing the reduction of hemoglobin of the present invention is a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode as in the prior art, and is characterized in that the working electrode is made according to the following steps:
[0022] a. First, according to the existing technology, utilize bromide to modify the electrode surface, use the glassy carbon electrode as the working electrode, the silver / silver chloride electrode as the reference electrode, and the platinum electrode as the counter electrode, and the working electrode, the reference electrode and the counter electrode The three-electrode system composed of electrodes is placed in a pH5.0 sodium acetate-acetic acid buffer solution containing graphene and toluidine blue, and the amount ratio of the graphene, toluidine blue to pH5.0 sodium acetate-acetic acid buffer solution is 10 μg : 0.5 mmol: 1L; In the potential ...
Embodiment 3
[0025] The electrochemical sensor capable of catalyzing the reduction of hemoglobin of the present invention is a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode as in the prior art, and is characterized in that the working electrode is made according to the following steps:
[0026] a. First, according to the existing technology, utilize bromide to modify the electrode surface, use the glassy carbon electrode as the working electrode, the silver / silver chloride electrode as the reference electrode, and the platinum electrode as the counter electrode, and the working electrode, the reference electrode and the counter electrode The three-electrode system composed of electrodes is placed in a pH5.0 sodium acetate-acetic acid buffer solution containing graphene and toluidine blue, and the amount ratio of the graphene, toluidine blue to pH5.0 sodium acetate-acetic acid buffer solution is 20 μg : 0.8 mmol: 1L; In the potential ...
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