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Catalytic reduction hemoglobin electrochemical sensor

A hemoglobin, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problem of narrow detection range, and achieve the effects of sensitive detection, simple preparation and low cost

Inactive Publication Date: 2016-08-17
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the above electrochemical sensors can detect hemoglobin very well, their detection range is narrow

Method used

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Experimental program
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Effect test

Embodiment 1

[0017] The electrochemical sensor capable of catalyzing the reduction of hemoglobin of the present invention is a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode as in the prior art, and is characterized in that the working electrode is made according to the following steps:

[0018] a. First, according to the existing technology, use bromide to modify the electrode surface, use the glassy carbon electrode as the working electrode, the saturated calomel electrode as the reference electrode, and the platinum electrode as the counter electrode, and make the working electrode, reference electrode and counter electrode The three-electrode system is put into the pH5.0 sodium acetate-acetic acid buffer solution containing graphene and toluidine blue, and the dosage ratio of the graphene, toluidine blue and pH5.0 sodium acetate-acetic acid buffer solution is 3 μ g: 0.3 mmol: 1L; within the potential range of -0.8 to 1.3V, 25 cycl...

Embodiment 2

[0021] The electrochemical sensor capable of catalyzing the reduction of hemoglobin of the present invention is a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode as in the prior art, and is characterized in that the working electrode is made according to the following steps:

[0022] a. First, according to the existing technology, utilize bromide to modify the electrode surface, use the glassy carbon electrode as the working electrode, the silver / silver chloride electrode as the reference electrode, and the platinum electrode as the counter electrode, and the working electrode, the reference electrode and the counter electrode The three-electrode system composed of electrodes is placed in a pH5.0 sodium acetate-acetic acid buffer solution containing graphene and toluidine blue, and the amount ratio of the graphene, toluidine blue to pH5.0 sodium acetate-acetic acid buffer solution is 10 μg : 0.5 mmol: 1L; In the potential ...

Embodiment 3

[0025] The electrochemical sensor capable of catalyzing the reduction of hemoglobin of the present invention is a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode as in the prior art, and is characterized in that the working electrode is made according to the following steps:

[0026] a. First, according to the existing technology, utilize bromide to modify the electrode surface, use the glassy carbon electrode as the working electrode, the silver / silver chloride electrode as the reference electrode, and the platinum electrode as the counter electrode, and the working electrode, the reference electrode and the counter electrode The three-electrode system composed of electrodes is placed in a pH5.0 sodium acetate-acetic acid buffer solution containing graphene and toluidine blue, and the amount ratio of the graphene, toluidine blue to pH5.0 sodium acetate-acetic acid buffer solution is 20 μg : 0.8 mmol: 1L; In the potential ...

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Abstract

The invention discloses a catalytic reduction hemoglobin electrochemical sensor which can achieve fast and sensitive detection of hemoglobin in a relatively large concentration range; the electrochemical sensor is a three-electrode system composed of a working electrode, a reference electrode and a counter electrode; the working electrode is prepared by the method according to the following steps: putting a three-electrode system with a glassy carbon electrode as the working electrode into a sodium acetate-acetic acid buffer solution having the pH of 5.0 and containing graphene and toluidine blue, and in a potential range of -0.8 to 1.3 V, carrying out cyclic voltammetry scanning for 25 laps at the scanning speed of 100 mV / s, to prepare a graphene / polytoluidine blue modified electrode; and putting a three-electrode system with the prepared graphene / polytoluidine blue modified electrode as the working electrode into an aqueous solution containing chloroplatinic acid and sodium chloride, and applying a -0.7 to -0.8 V constant potential between the working electrode and the reference electrode with the application time of 60-240 s.

Description

technical field [0001] The invention relates to an electrochemical sensor, in particular to an electrochemical sensor which can realize rapid and sensitive detection of hemoglobin in a relatively large concentration range and can catalyze the reduction of hemoglobin. Background technique [0002] Hemoglobin is an important protein in higher organisms. It is responsible for carrying oxygen, participating in the transportation of carbon dioxide in the blood and the regulation of blood pH. Clinically, the detection of hemoglobin can provide diagnosis for lung diseases, cardiovascular diseases and some tumor diseases Therefore, it is of great significance to quickly and sensitively detect the content of hemoglobin in blood. In the past, the commonly used detection methods for hemoglobin include radioimmunoassay, enzyme-linked immunoassay, etc. These methods require the preparation of biological antibodies, and the specific reaction between hemoglobin and biological antibodies (i...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 孙越兰玉廷张家萌李娟杜洪莹冯春梁
Owner LIAONING NORMAL UNIVERSITY
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