Method for observing microscopic structures inside plant roots

Abstract

The invention relates to a method for observing microscopic structures inside plant roots, which is capable of effectively solving the problem of utilizing a microscope to clearly observe the structures inside the plant roots. The method comprises the steps of digging out the plant roots, washing, shearing the plant roots into small sections, fixing the plant roots by methanol, and then embedding by a sepharose solution, cooling, condensing, trimming into small blocks, after freehand section, placing the small blocks into distilled water in a container, performing lactic acid clearing, covering, dyeing via berberine or fluorescein, bleaching by the water, performing poststaining via toluidine blue O or sarranine O, bleaching by the water, performing water seal, observing and photographing via an inverted fluorescence microscope, and obtaining a clear image. The method can be used for observing the microscopic structures inside the plant roots, thereby being effectively used for the research on classification, auxanology, ecology and physiology of medical plants. The method disclosed by the invention has the advantages of simple method, easiness in operation, low cost, low working capacity, high success rate, and good effect, thereby being capable of being used for the research on morphological anatomy of the roots. The method is simpler and more effective for the thinner root sections of the plants.

Description

technical field [0001] The invention relates to a method for observing the internal microstructure of the plant root by using a freehand slicing technique to slice the root of the plant, undergo transparent and dyeing treatments, and use an optical microscope to observe the microstructure of the plant root. Background technique [0002] Freehand sectioning of fresh plant tissue allows the microstructure of the plant interior to be observed with an optical microscope. Many medicinal plants use rhizomes as their medicinal parts. The study of root morphology and anatomy is very important for the classification, development, ecology, and physiology of medicinal plants. The acquisition of high-definition microstructure images must first obtain high-quality slices, and then undergo appropriate transparent and staining treatments. [0003] Since fresh plant material is difficult to preserve for a long time, plant roots can be preserved only after being fixed. However, the current...

AI Technical Summary

Problems solved by technology

However, current fixation methods tend to cause softening and shrinkage of plant tissue, resulting in poor slice quality

The commonly used fixative uses formaldehyde, which will cause strong autofluorescence of the cell wall of plant roots under ultraviolet light, and also affect the dyeing of fluorescent dyes, making it difficult to clearly observe the internal structure of roots

In the current freehand sectioning, chloral hydrate is usually used for transparent treatment of plant slices. Practice has proved that the slices of plant roots after chloral hydrate treatment are still not clear enough to observe under an optical microscope.

It is very difficult to slice the fine roots of plants. The frozen section technique needs to be completed with an expensive frozen section machine. Difficulty penetrating into plant material so that internal root structures such as inner cortex, outer cortex, Caspian bands, etc. cannot be clearly observed