Screening method of high-performance hydrolytic bacterial strain of waste Baijiu distiller grains

A screening method and technology of liquor, applied in the field of screening and research of complex bacterial groups, can solve the problems of low usable value, high acidity of discarded grains, pollution of the environment, etc., and achieve the effect of promoting development and utilization

Inactive Publication Date: 2018-11-30
GUIZHOU MEDICAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention: In view of the situation of high acidity, low nutrition, low usable value, and environmental pollution of discarded grains, provide a kind of high-efficiency microbial group to improve the hydrolysis rate of discarded grains, reduce the storage...

Method used

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  • Screening method of high-performance hydrolytic bacterial strain of waste Baijiu distiller grains
  • Screening method of high-performance hydrolytic bacterial strain of waste Baijiu distiller grains
  • Screening method of high-performance hydrolytic bacterial strain of waste Baijiu distiller grains

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Embodiment Construction

[0038]First, the Westerdijk Research Center purchases the fungi that hydrolyze the distilled grains of liquor, and isolates the fungal strains from the acidic environment of the distilled grains;

[0039] 1. Sampling in the acidic environment of the liquor enterprise (collecting liquor dregs and fresh liquor dregs in the winery, distiller dregs are used to isolate fungi that may have hydrolyzed liquor dregs, fresh liquor dregs are used to add into the culture medium), weigh 10 g of compost samples into a 250 mL Erlenmeyer flask, add 100 mL of sterile water, and shake in a 30°C constant temperature shaking box for 3 to 4 hours.

[0040] 1.1 Isolation of strains: Let the uniformly shaken bacterial solution stand for 30 minutes, draw an appropriate amount of supernatant for 10-fold gradient dilution, and draw 10 -1 ~10 -5 Inoculate 0.5 mL of each gradient bacterial solution on YPD medium (yeast extract peptone glucose agar medium, containing chloramphenicol), and incubate at 28°...

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Abstract

The invention discloses a screening method of a high-performance hydrolytic bacterial strain of waste Baijiu distiller grains. The screening method is characterized by comprising the following steps:step I, directly separating and culturing fungus from effective fungus that possibly hydrolyzes waste Baijiu distiller grains of relevant waste hydrolytic Baijiu distiller grains in a bacterial strainlibrary in a waste Baijiu distiller grains stacking environment; step II, performing the primary screening for the screened fungus successively by virtue of a sodium carboxymethylcellulose-Congo redculture medium, PDA-aniline blue culture medium, and a xylan-Congo red culture medium by adopting the single polymer which is difficult to hydrolyze in the waste Baijiu distiller grains as an exclusive carbon source; step III, culturing the bacterial strain in a waste distiller grain solution adopting the waste Baijiu distiller grain as an exclusive nutritional substance, and determining the capability of the bacterial strain for the waste distiller grains; step IV, performing the enzyme activity test for the composite bacterial strain in the waste Baijiu distiller grain solution, and screening the bacterial group with synergistic effect and high enzyme activity, thus obtaining the high-performance composite hydrolytic bacterial group; and step V, comparing enzyme activity and biomass hydrolytic capability of the high-performance composite bacterial strain with that of the disclosed mutant bacterial strain having the high-performance hydrolytic cellulose.

Description

technical field [0001] The present invention relates to the optimization research of the scheme of hydrolyzing liquor and discarding grains, in particular to the screening research of two groups of new high-efficiency hydrolyzing liquor and discarding grains compound bacterial groups. technical background [0002] Liquor distilled grains are the husks and residues of grains that are poor in nutrition after repeated fermentation and distillation to extract alcohol. That is, the content of distiller's grains is high in moisture and lignocellulose (cellulose, hemicellulose, lignin), and the content of crude protein and crude fat is low. Because the lost grains have less nutrients and lignocellulose is difficult to hydrolyze, it cannot be as effective as ordinary distiller's grains Therefore, the transformation of waste waste is greatly restricted. Moreover, the waste waste has high acidity and high moisture content, which is very easy to spoil and deteriorate, causing pollutio...

Claims

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Application Information

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IPC IPC(8): C12N1/02C12Q1/04
CPCC12N1/02C12Q1/04
Inventor 康颖倩罗鹏
Owner GUIZHOU MEDICAL UNIV
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