Beta-dextran detection method based on fluorescence enhancement principle

A technology of fluorescence enhancement and detection method, which is applied in the field of fluorescence enhancement and achieves the effects of simple and effective method, wide application range and simple preparation device

Inactive Publication Date: 2016-08-10
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] So far, no researchers have combined the fluorescence enhancement effect of one-dimensional photonic crystals with the metal surface enhancement effect, and used the combined fluorescence enhancement effect to detect guest molecules

Method used

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  • Beta-dextran detection method based on fluorescence enhancement principle
  • Beta-dextran detection method based on fluorescence enhancement principle

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Dip the silicon wafer in acetone solution for 30 minutes, then immerse it in ethanol solution for 30 minutes, and finally immerse it in H2O with a volume ratio of 1:3 2 SO 4 / H 2 o 2 Soak in the mixed solution for 12h, N 2 Blow dry to obtain a clean blank silicon wafer.

[0040] 2) Weigh 4g of tetrabutyl titanate and add it to 16ml of ethanol, slowly add 2ml of glacial acetic acid dropwise under magnetic stirring, and stir at room temperature for 5h to obtain light yellow transparent TiO with a concentration of 39mg / ml. 2 colloidal solution. Dissolve GO in water to make a GO colloid solution with a concentration of 0.25 mg / ml, pour the prepared 0.25 mg / mL GO hydrogel into a high-pressure reactor, and react at 90 ° C for 17 hours to obtain a concentration of 0.25 mg / ml. ml of GO hydrogel.

[0041] 3) TiO layer by layer 2 The colloidal solution and GO hydrogel were spin-coated onto the surface of the substrate, the spin-coating speed was 4500rpm, the spin-coatin...

Embodiment 2

[0049] 1) Dip the silicon wafer in acetone solution for 30 minutes, then immerse it in ethanol solution for 30 minutes, and finally immerse it in H 2 SO 4 / H 2 o 2 Soak in the mixed solution for 4h, N 2 Blow dry to obtain a clean blank silicon wafer.

[0050] 2) Weigh 4g of tetrabutyl titanate and add it to 16ml of ethanol, slowly add 2ml of glacial acetic acid dropwise under magnetic stirring, and stir at room temperature for 6h to obtain TiO with a concentration of 39mg / ml. 2 colloidal solution, and then diluted 3-fold to obtain a TiO concentration of 13 mg / ml 2 colloidal solution. Dissolve GO in water to make a GO colloid solution with a concentration of 0.5 mg / ml, pour the prepared 0.5 mg / mL GO hydrogel into a high-pressure reactor, and react at 90 ° C for 18 hours to obtain a concentration of 0.5 mg / ml. ml of GO hydrogel.

[0051] 3) TiO layer by layer 2 The colloidal solution and GO hydrogel were spin-coated onto the surface of the substrate, the spin-coating spe...

Embodiment 3

[0057] 1) Dip the silicon wafer in an acetone solution for 60 minutes, then immerse it in an ethanol solution for 60 minutes, and finally immerse it in H2O with a volume ratio of 3:7. 2 SO 4 / H 2 o 2 Soak in the mixed solution for 12h, N 2 Blow dry to obtain a clean blank silicon wafer.

[0058] 2) Weigh 4g of tetrabutyl titanate and add it to 16ml of ethanol, slowly add 2ml of glacial acetic acid dropwise under magnetic stirring, and stir at room temperature for 5h to obtain TiO with a concentration of 39mg / ml. 2 colloidal solution, and then diluted 5-fold to obtain a concentration of 7.8 mg / ml TiO 2 colloidal solution. Dissolve GO in water to prepare a GO colloid solution with a concentration of 0.25 mg / ml. Pour the prepared 0.25 mg / mL GO hydrogel into an autoclave and react at 100°C for 17 hours to obtain a concentration of 0.25 mg / ml. ml of GO hydrogel.

[0059] 3) TiO layer by layer 2 The colloidal solution and GO hydrogel were spin-coated onto the surface of the ...

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Abstract

The invention discloses a method for detecting β-glucan based on the principle of fluorescence enhancement. The treated silicon wafer is used as the substrate, and titanium dioxide and graphene oxide hydrogel are deposited on the silicon wafer in combination with spin-coating technology to prepare titanium dioxide/ Graphene oxide hydrogel one-dimensional photonic crystal, and gold-plated on the photonic crystal by vacuum evaporation method, prepared gold-plated titanium dioxide/graphene oxide hydrogel one-dimensional photonic crystal, and used this photonic crystal as a fluorescence-enhanced Substrate, a method for detecting β-glucan by detecting the fluorescence intensity of the fluorescent complex product specifically combined with aniline blue. The present invention uses gold-plated titanium dioxide/graphene oxide hydrogel one-dimensional photonic crystals as the base, and combines the fluorescence enhancement effects of gold nanoparticles and photonic crystals to cause greater fluorescence enhancement, thereby improving the sensitivity of fluorescence detection. The method is simple and effective, the operation is convenient and the required time is short.

Description

technical field [0001] The invention relates to the technical field of fluorescence enhancement, in particular to a method for preparing a gold-plated titanium dioxide / graphene oxide hydrogel one-dimensional photonic crystal. Background technique [0002] Photonic crystals can be regarded as regular structures formed by periodic arrangement of materials with different dielectric constants. [0003] Yablonovitch and John were the first to work on the design of photonic crystals. According to periodicity, photonic crystals can be divided into one-dimensional, two-dimensional and three-dimensional photonic crystals. Photonic crystals have different emission wavelengths, which are determined by the distance between the layers, which also contributes to their unique colors. When the periodicity of the photonic crystal changes, for example, when a chemical stimulus is encountered, the reflection wavelength of the photonic crystal will also change. This provides a convenient ave...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 葛丽芹任姣雨宣红云
Owner SOUTHEAST UNIV
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