Method for identifying self-fruitful pear varieties by using pollination in vitro

Abstract

The invention discloses a method for identifying self-fruitful pear varieties by using pollination in vitro indoor, belonging to the field of plant breeding. The method comprises the following steps of: carrying out self-pollination by using large flower buds; immediately cutting styluses from the base parts of the styluses and the ovaries by using blades after carrying out the self-pollination; placing the styluses on a solid medium, culturing the styluses for 48 hours under the dark environment of 25DEG C, and taking out the styluses; dripping several drips of 0.1 percent aniline blue staining fluid on the base parts of the styluses; and observing the grow of pollen tubes from the base parts of the styluses under a microscope. If the pollen tubes grow from the base parts of the styluses, the variety can be fruitful by using the self-pollination; and if the pollen tubes do not grow from the base parts of the styluses, the variety cannot be fruitful by using the self-pollination. The method solves the problem of identifying the self-fruitful property in large quantity within one year, not only can identify all pear variety resources, but also can reference the identification of the self-fruitful property of other fruit trees and has wide application prospect.

Description

1. Technical field [0001] The invention provides a set of in vitro pollination style culture methods to identify pear self-fertile varieties or strains, and belongs to the field of plant breeding. 2. Background technology [0002] Pear is one of the main fruits in my country and even in the world. my country's existing cultivated area is 1,258,400 hectares, and it is the third largest fruit in my country. Pear is a typical gametophytic self-incompatibility fruit tree, that is to say, most varieties of pear are self-pollinating and not fruiting. In production, pollination trees must be rationally allocated or artificially assisted pollination can be obtained. Yield is also often reduced due to improper configuration of pollination trees, bad meteorological conditions during the flowering period affecting insect pollination or untimely artificial pollination, etc., especially in recent years. Many pear trees have poor pollination and fertilization, and the fruit setting rate ...

AI Technical Summary

Problems solved by technology

This method has changed the time-consuming, labor-intensive and inefficient situation of determining the degree of self-fertilization of fruit tree varieties only by the rate of artificial self-pollination in the field for a long time.

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