Method for identifying self-fruitful pear varieties by using pollination in vitro

A technology for identifying pears and varieties, applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve the problems of high fruit setting rate, time-consuming, labor-intensive, low efficiency, etc. Easy-to-use effects

Inactive Publication Date: 2010-11-24
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

This method has changed the time-consuming, labor-intensive and inefficient situation of determining the degree of...

Method used

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  • Method for identifying self-fruitful pear varieties by using pollination in vitro
  • Method for identifying self-fruitful pear varieties by using pollination in vitro
  • Method for identifying self-fruitful pear varieties by using pollination in vitro

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Embodiment Construction

[0022] (1) Experimental method

[0023] 1. Hydroponic flower promotion: The 52 varieties tested are all from the pear germplasm resource nursery of the Jiangpu Horticultural Experimental Station of Nanjing Agricultural University (the Institute of Pomology, Chinese Academy of Agricultural Sciences, these varieties are preserved in the National Pear Germplasm Resource Garden, detailed information See the Registration Form for Disclosure of Patented Genetic Resource Sources), in the first ten days of February before spring flowering, cut the robust branches with plump flower buds that have been released from dormancy by low temperature, cut the base of the branches into an inclined plane, and insert them upright in an aqueous solution containing 10% sucrose In a bucket with a depth of 5-6 cm, replace the solution once every 5 days, and put it in a greenhouse or artificial climate room to promote flowering;

[0024] 2. Self-pollination: Take a complete inflorescence, remove the b...

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Abstract

The invention discloses a method for identifying self-fruitful pear varieties by using pollination in vitro indoor, belonging to the field of plant breeding. The method comprises the following steps of: carrying out self-pollination by using large flower buds; immediately cutting styluses from the base parts of the styluses and the ovaries by using blades after carrying out the self-pollination; placing the styluses on a solid medium, culturing the styluses for 48 hours under the dark environment of 25DEG C, and taking out the styluses; dripping several drips of 0.1 percent aniline blue staining fluid on the base parts of the styluses; and observing the grow of pollen tubes from the base parts of the styluses under a microscope. If the pollen tubes grow from the base parts of the styluses, the variety can be fruitful by using the self-pollination; and if the pollen tubes do not grow from the base parts of the styluses, the variety cannot be fruitful by using the self-pollination. The method solves the problem of identifying the self-fruitful property in large quantity within one year, not only can identify all pear variety resources, but also can reference the identification of the self-fruitful property of other fruit trees and has wide application prospect.

Description

1. Technical field [0001] The invention provides a set of in vitro pollination style culture methods to identify pear self-fertile varieties or strains, and belongs to the field of plant breeding. 2. Background technology [0002] Pear is one of the main fruits in my country and even in the world. my country's existing cultivated area is 1,258,400 hectares, and it is the third largest fruit in my country. Pear is a typical gametophytic self-incompatibility fruit tree, that is to say, most varieties of pear are self-pollinating and not fruiting. In production, pollination trees must be rationally allocated or artificially assisted pollination can be obtained. Yield is also often reduced due to improper configuration of pollination trees, bad meteorological conditions during the flowering period affecting insect pollination or untimely artificial pollination, etc., especially in recent years. Many pear trees have poor pollination and fertilization, and the fruit setting rate ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
Inventor 张绍铃齐永杰吴俊吴华清陶书田齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY
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