Application of rice OsNDPK1 gene in the aspect of improvement of plant disease resistance

A disease resistance, rice technology, applied in the field of genetic engineering, can solve the problem that the role of NDPK is not very clear, and achieve the effect of improving plant disease resistance and increasing yield

Inactive Publication Date: 2013-11-13
HEBEI AGRICULTURAL UNIV.
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the species that have cloned the NDPK gene include spinach (Spinacia oleracea) (Nomura T, et al., 1992), pea (pisum sativum) (Finan P M, et al., 1994), tomato (Lycopersicon esculentum) (Harris N, et al., 1994), oat (avena sativa) (Sommer D, et al., 1994), rice (Oryza sativa) (Yano A, et al., 1993), etc., but NDPK is involved in plant growth and development The role of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of rice OsNDPK1 gene in the aspect of improvement of plant disease resistance
  • Application of rice OsNDPK1 gene in the aspect of improvement of plant disease resistance
  • Application of rice OsNDPK1 gene in the aspect of improvement of plant disease resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Overexpression OsNDPK1 Acquisition and Disease Resistance Analysis of Transgenic Arabidopsis

[0024] OsNDPK1 The CDS region of the gene was first cloned on the pMD-19 simple vector, and then used Kpn I and Bam HI will OsNDPK1 The CDS region of the gene is excised and applied Kpn I and Bam HI will OsNDPK1 The CDS region of the gene was connected to the vector pCAMBIA1300 Kpn I and Bam between HI restriction sites. The vector contains the CaMV 35S promoter, which can drive the overexpression of the target gene. contain OsNDPK1 The pCAMBIA1300 vector of the CDS region of the gene was transformed into Arabidopsis thaliana by the method of Agrobacterium dipping flowers. Overexpressed by RT-PCR analysis OsNDPK1 Positive plants ( figure 1 , 2 ).

[0025] will overexpress OsNDPK1 The strains of Arabidopsis and wild-type Arabidopsis were cultured for 4 weeks under long-day light and inoculated with needle-free syringes. Pst DC3000 bacterial strain 2 day...

Embodiment 2

[0029] Expression analysis of disease resistance-related genes

[0030] Inoculation of BC22 with wild-type and overexpressed OsNDPK1 After 0, 1, 3, 6, 12, and 24 hours of the transgenic Arabidopsis, RT-PCR analysis revealed that the wild-type Arabidopsis chitinase gene Cha1 The expression level decreased 3h after inoculation, while overexpression OsNDPK1 transgenic Arabidopsis Cha1 Gene expression can be continuously expressed ( Figure 7 ), which shows that the overexpression OsNDPK1 The transgenic Arabidopsis thaliana can continue to resist the infection of pathogenic bacteria; at the same time, the inventors analyzed the resistance-related genes NPR1 with PR1 The expression changes, it was found that the overexpression OsNDPK1 transgenic Arabidopsis NPR1 with PR1 The expression levels were significantly higher than those of wild-type Arabidopsis, NPR1 Genes are particularly pronounced ( Figure 8 ). NPR1 Genes play a key role in plant systemic acquired re...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The occurrence of diseases is an important factor restricting crop high yield. The fields with diseases usually have yields reduced by 20%-30%, the fields with serious diseases have the yields reduced by 50%-60%, and serious economic losses are caused. According to the invention, an over expression vector of OsNDPK1 gene (with the gene serial number of Os07g0492000) is constructed, and an over expression mutant of the OsNDPK1 gene is obtained through agrobacterium transformation. Experiments show that: the OsNDPK1 gene can improve resistance of plants to bacteria PstDC3000 and fungus botrytis cinerea BC22, can regulate generation of callose in plant bodies, and can induce expression of anti-disease genes NPR1 and PR1. Analysis and identification of OsNDPK1 gene functions provide theoretical basis and production and practice bases for obtaining of new varieties of anti-disease transgenic plants.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to a rice OsNDPK1 Application of genes in improving plant disease resistance. Background technique [0002] NDPK (nucleoside diphosphate kinase) widely exists in animals, fungi, plants and prokaryotes. NDPK is a phosphorylated protein whose main function is to maintain the intracellular NTP concentration by catalyzing the transfer reaction of phosphate groups between NDP and NTP. Provide raw materials for nucleic acid synthesis (Sonia D, et al., 2006). There are three kinds of NDPKs in plants, NDPK1 is the main form, which exists in the cytoplasm, accounting for about 70% of the total NDPKs, and has a higher expression level in the meristem (Dorion S, et al., 2006 ). NDPK2 is mainly involved in some signaling pathways, related to phytochrome (Matsushita Y, et al., 2002). NDPK3 mainly exists in the mitochondrial intermembrane space and interacts with adenosine nucleic acid transpor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54A01H5/00
Inventor 王凤茹董金皋司贺龙时翠平李生辉贾慧
Owner HEBEI AGRICULTURAL UNIV.
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap