Application of rice OsNDPK1 gene in the aspect of improvement of plant disease resistance

Abstract

The occurrence of diseases is an important factor restricting crop high yield. The fields with diseases usually have yields reduced by 20%-30%, the fields with serious diseases have the yields reduced by 50%-60%, and serious economic losses are caused. According to the invention, an over expression vector of OsNDPK1 gene (with the gene serial number of Os07g0492000) is constructed, and an over expression mutant of the OsNDPK1 gene is obtained through agrobacterium transformation. Experiments show that: the OsNDPK1 gene can improve resistance of plants to bacteria PstDC3000 and fungus botrytis cinerea BC22, can regulate generation of callose in plant bodies, and can induce expression of anti-disease genes NPR1 and PR1. Analysis and identification of OsNDPK1 gene functions provide theoretical basis and production and practice bases for obtaining of new varieties of anti-disease transgenic plants.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to a rice OsNDPK1 Application of genes in improving plant disease resistance. Background technique [0002] NDPK (nucleoside diphosphate kinase) widely exists in animals, fungi, plants and prokaryotes. NDPK is a phosphorylated protein whose main function is to maintain the intracellular NTP concentration by catalyzing the transfer reaction of phosphate groups between NDP and NTP. Provide raw materials for nucleic acid synthesis (Sonia D, et al., 2006). There are three kinds of NDPKs in plants, NDPK1 is the main form, which exists in the cytoplasm, accounting for about 70% of the total NDPKs, and has a higher expression level in the meristem (Dorion S, et al., 2006 ). NDPK2 is mainly involved in some signaling pathways, related to phytochrome (Matsushita Y, et al., 2002). NDPK3 mainly exists in the mitochondrial intermembrane space and interacts with adenosine nucleic acid transpor...

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Problems solved by technology

So far, the species that have cloned the NDPK gene include spinach (Spinacia oleracea) (Nomura T, et al., 1992), pea (pisum sativum) (Finan P M, et al., 1994), tomato (Lycopersicon esculentum) (Harris N, et al., 1994), oat (avena sativa) (Sommer D, et al., 1994), rice (Oryza sativa) (Yano A, et al., 1993), etc., but NDPK is involved in plant growth and development The role of

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