Method for rapidly detecting wheat tissue callose

A detection method, callose technology, is applied in the field of rapid detection of wheat tissue callose, which can solve the problems of expensive equipment, affecting the production effect, and difficulty in mastering the technology, and achieves easy mastery, strong operability, and relative content difference obvious effect
CN103149189AInactive Publication Date: 2013-06-12CROP RES INST SHANDONG ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CROP RES INST SHANDONG ACAD OF AGRI SCI
Publication Date
2013-06-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for dyeing and observing wheat tissue callose and belongs to the technical field of chemical plant tissue positioning. The method mainly comprises the following steps: (1) preparing a solution; (2) treating the material; (3) slicing; (4) dyeing materials; (5) producing sections; and (6) exciting through a laser excitation device, and observing and shooting through a fluorescence microscope. By adoption of a bare-handed slicing method, the wheat tissue slices which are manufactured in a bare-handed mode are dyed under the alkaline conditions by using the callose specifity fluorescent dye-water-soluble aniline blue, the sample slice manufacturing, dyeing and observing period is obviously shortened, and the callose is accurately positioned. The method is high in operability, the manufactured slices are conveniently observed, and the acquired image is clear.
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Description

technical field

[0001] The invention relates to the technical field of microscopic preparation of plant tissue and chemical localization of tissue components, in particular to a rapid detection method for callose in wheat tissue. Background technique

[0002] Callose is a kind of glucan bound by β-1,3-bonds, which plays an important role in regulating plant life activities such as sieve tube metabolism and gametophyte development. Its synthesis and decomposition are directly related to the normal growth and metabolism of plants. process. In addition, when plants are subjected to adverse environmental stress or strong stimuli, such as pathogenic bacteria infection, nutritional stress, mechanical damage, high temperature and other adversities, callose metabolism will immediately feedback and regulate the physiological activities of plant cells (Wang, McCallum, Fetch et al. 2012; Chapman et al., 2009; Saulnier et al., 2012). Under adversity, callose will be quickly synthesize...

Claims

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