The invention relates to a sterilization composition containing probenazole and methoxy acrylates, comprising an active component A, and an active component B, wherein the active component A is selected form probenazole, and the active component B is selected form one of kresoxim methyl, azoxystrobin, pyraclostrobin, trifloxystrobin, kresoxim-methyl, dimoxystrobin, fluoxastrobin, orysastrobin, picoxystrobin, kresoxim-methyl and pyraoxystrobin, wherein a mass ratio of the active component A to the active component B is 80:1-1:60. The composition can be prepared to be wettable powder, water dispersible granules, a suspending agent, a suspoemulsion, an emulsion in water, a micron emulsion, a microcapsule suspending agent or a microcapsule-microcapsule suspending agent, for treating powdery mildew, rice blast, false smut, sheath blight, leaf spot, black spot, rust disease, alternaria leaf spot, bakanae disease, downy mildew, septoria nodorum, net blotch, anthracnose, leaf mold, scab, leaf blight, and tan disease. The composition resists rainwater washing out, and has obvious synergism.