54 results about "Isomalto oligosaccharides" patented technology

Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.

Disclosed are food products, in particular sports drinks and energy bars, that include isomalto-oligosaccharides. In one embodiment, at least 35% of the nutritive carbohydrate content of the food product comprise an isomalto-oligosaccharide. In another embodiment, at least 35% of the caloric content of the food product is attributed to the isomalto-oligosaccharide. In another embodiment, the isomalto-oligosaccharide is present in an amount effective to provide a nutritive caloric content of at least 50 kcal.

The invention relates to a composition containing oligosaccharide and an anabasine insecticide, wherein the oligosaccharide is one or more selected from chitosan oligosaccharide, sodium alginate oligosaccharides, pectin oligosaccharide, oligosaccharides, xylo-oligosaccharide and soybean oligosaccharide, and the anabasine insecticide is one or more selected from imidacloprid, acetamiprid, thiamethoxam, thiacloprid, nitenpyram, dinotefuran and clothianidin. The mass proportion of the oligosaccharide to the anabasine insecticide is (40:1)-(1:40). Compared with the prior art, for the pesticide composition disclosed by the invention, a composition of active ingredients has a synergistic effect; and compared with anabasine singe dosages, for the pesticide composition disclosed by the invention, the effects on aphid, aleyrodid, plant hopper and the like are obviously improved.

The invention provides a cookie for correcting the deviation of simple obesity. The cookie comprises various minor crops, medicinal and edible raw materials and diet fibers and has a unique function, which cannot be replaced by medicines, for correcting the simple obesity caused by overhigh heat taken in by modern people because of diet deviation. The cookie consists of wheat, sheep eye beans, lily beans, circular and flat beans, wheat fibers, konjaku flour, kelp, cordate houttuynia, honeysuckle, siraitia grosvenorii, bunge cherry seed, semen cassiae, hawthorn, fuling, yam, chrysanthemum, semen raphani, lotus leaves, lotus seeds, malt, dried ginger, liquorice, spina date seeds, peach kernel, almond, fructus lycii, orange skin, lily, scaphium scaphigerum, 500-type isomalto oligosaccharides, soybean phospholipids and a swelling agent. The cookie is processed by mixing the raw materials according to ruled proportions. The manufacturing technology is simple; and the cookie has wide application prospect.

The invention discloses a compound synbiotics preparation and its application. The compound synbiotics preparation includes composite Probiotics and Prebiotics, the composite Probiotics comprise Bacillus subtilis and Bacillus licheniformis, the Prebiotics are anyone of yeast cell wall polysaccharides, mannan oligosaccharides, fructo-oligosaccharides, soybean oligosaccharides, isomalto oligosaccharides, lactosucrose, galactooligosaccharides, sucrose thermal oligosaccharides caramel and oligonucleotides xylose; and the survival rate of spores is higher than 90% after the mixing granulation of the compound synbiotics preparation and a feed, and the total number of live bacteria is 1.0*10<9>-1.0*10<11>CFU/g. By utilizing the cooperative effect of different Probiotics and Prebiotics, the compound synbiotics preparation avoids the disadvantages of single Probiotics, increases the content of the Probiotics, reduces the competitor rate, improves the heat resistance and the storability of the synbiotics, improves the quality of feeding synbiotics, and has a wide application prospect.

A method of improving the health and production of ruminant animals, the method including the step of orally administering to the animal an effective amount of an indigestible oligosaccharide selected from the group of gluco-oligosaccharides and isomalto-oligosaccharides.

The invention relates to an antrodia mycelium flavor soft sweet and a processing method thereof. The main raw material comprises the following components by mass: 10%-27% of fresh antrodia mycelium, 10%-20% of white granulated sugar, 0-20% of glucose syrup, 16%-30% of isomalto oligosaccharide, 20%-30% of malt dextrin, 2%-6% of agar and 1%-3% of carrageenan, and accessories (taking the total weight of the main raw material as a basis for calculating) comprise 0.1%-0.3% of citric acid, 0.5%-2% of Vc, 0.2%-0.8% of sodium citrate and 0.02%-0.1% of potassium sorbate, before the end of sugaring off, when the sugar paste concentration is 75%, antrodia mycelium pellet is added for forming and drying. The characteristics of the processing method is scientific adding of the fresh antrodia mycelium and the isomalto oligosaccharide, the health function of the soft sweet is increased, the soft sweet quality is improved, the amount of added sugar is reduced, the antrodia mycelium flavor soft sweet is suitable for the majority of people to eat, expands consumer groups, and can meet the needs of consumer groups in the elderly.

The invention provides alpha-glucosidase. The amino acid sequence of the alpha-glucosidase is SEQ ID NO:1. The optimum action temperature of the alpha-glucosidase is 60 DEG C; over 80% of enzyme activity can be retained in a range of 50-65 DEG C; the optimum action pH value is 4.5; over 80% of enzyme activity can be retained in a pH range of 3.0-6.0. The alpha-glucosidase is capable of efficiently converting maltose; the content of isomaltose, the content of panose and the content of isomaltotriose in a conversion product are the highest and respectively reach 44.1%, 23.0% and 21.2%; the total content of the isomaltose, the panose and the isomaltotriose is 88.3%. Therefore, the alpha-glucosidase can be widely applied to production of isomalto oligosaccharides and has a wide market prospect.

The invention provides a novel composite probiotic preparation for a pig-raising fermentation bed, wherein the preparation comprises the following components: bacillus subtilis, anaerobic bacteroid, lactobacillus fermenti, bifidobacteria, lactobacillus, saccharomycetes, fructooligosaccharide, isomalto-oligosaccharide, DL-methionine, L-lysine, multi-vitamin, and soybean powder. The probiotics, which are used for the preparation of the ecological pig-raising fermentation bed, propagate and grow well in organic materials such as straw, rich husk, and the like, can strengthen the decomposition efficiency of pig manure and urine, have a stable number of bacterial colonies in the culture period, effectively prolong the service life of the fermentation bed, at the same time help to modulate the microecological balance in the pig intestinal tracts, improve the immunity function and productive performance of ecologically-fed pigs, and prominently increase the economic profit of pig feeding.

The invention relates to health food and in particular relates to pine pollen tablets. The pine pollen tablets are prepared from the following raw materials by weight percent: 49%-90% of pine pollen and 10%-51% of isomalto-oligosaccharides. The pine pollen tablets have the effect of improving immunity; the pine pollen contains rich vitamin B and various enzymes, so that the gastrointestinal motility can be promoted and the digestive absorption function can be improved; the pine pollen has a very remarkable effect of protecting the liver and can prevent and treat the liver diseases, promote the liver cell activity and improve the detoxification function of the liver after being taken for a long term; the pine pollen also can effectively regulate the endocrine system and improve the body immunity, so that the endocrine system of a human body can be excited for self protection when pathogenic bacteria and harmful substances enter the body.

The invention discloses a formula of meal-replacement prebiotic milkshake (a prebiotic food). The formula of the meal-replacement prebiotic milkshake comprises the following ingredients in proportion:10-20% of of oat powder, 10-20% of wheat germs, 5-15% of psyllium husk, 5-10% of soybean protein isolate, 5-10% of soybean dietary fiber, 5-10% of whey protein, 5-10% of oat fiber, 5-20% of isomalto-oligosaccharides, 4-20% of inulin, 1-6% of stachyose, 1-2% of xylo-oligosaccharides, 2-8% of fructo-oligosaccharides, 2-3% of galacto-oligosaccharides, and 1-5% of soybean oligosaccharides. Compared with common protein powder and the three meals, the formula of the meal-replacement prebiotic milkshake is obvious in the effects of increasing frequency of defecation, deceasing weight and reducing waist circumference. By applying the effects of regulating intestinal flora, delaying gastric emptying, increasing stool volume and water content and the like of the prebiotics in formula improvement ofweight-reducing meal-replacement products, especially in formula improvement of meal-replacement milkshake, better use experience and weight-reducing effects are achieved.

The invention discloses a synbiotics composition, a synbiotics preparation, a preparation method and application thereof and relates to the technical field of microorganism additives. The synbiotics preparation prepared from the synbiotics composition is prepared from the following components in parts by weight: 400-500 parts of rice bran, 5-15 parts of lactic acid bacteria, 2-8 parts of enterococcus faecalis, 2-8 parts of enterococcus faecium, 160-240 parts of clostridium butyricum, 5-15 parts of bacillus subtilis, 290-340 parts of isomalto-oligosaccharides and 0-5 parts of a mildew preventive. According to the synbiotics composition, the technical problems of feeding synbiotics produced at present that the bacterium content is low, the content of contaminating microorganisms is high and the quality is not stable are solved; the synbiotics composition and the synbiotics preparation, disclosed by the invention, are cooperatively matched with a plurality of types of probiotics and prebiotics, and a gastrointestinal environment capable of maintaining body health is established and maintained; the growth speed of livestock and poultry is improved and the immunity of the organism is enhanced; and the diarrhea rate and the morbidity are reduced, and the production performance of the livestock and poultry is improved.

Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.

The invention relates to a method for synthesizing isomalto oligosaccharides through a fully enzymatic transformation path. The method includes the steps of slurry adjustment and liquefaction, saccharification, transglycosidation, decolorization, ion exchange, vacuum concentration, nanofiltration and separation and drying. Polysaccharides are transformed into the isomalto oligosaccharides in an enzymatic mode by the utilization of alpha-transglucosidase. According to the method, the polysaccharides are transformed effectively for production of the isomalto oligosaccharides. The technology is simple, operation is easy, and pH adjustment is not needed, so that the use amount of acid-base is reduced, and the load of ion exchange in the follow-up technologies is reduced. The transformation ratio of the finished product isomalto oligosaccharides is high, and a chemical synthesis method can be replaced by the method.

The invention provides a mutant enzyme having trans-sialidase activity (EC 3.2.1.18), characterized by an enhanced trans-sialidase:sialidase ratio when compared to its parent sialidase enzyme. Further the enzyme may be used in a method for trans-sialylating mono- and oligo-saccharides, including galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), malto-oligosaccharides (MOS), isomalto-oligosaccarides (IMO), lactulose, melibiose, maltose, glycosyl sucrose, lactosucrose and fucose. Trans-sialidated mono- and oligo-saccharides, produced with the mutant enzyme, are useful in preparing infant formula, a prebiotic nutritional supplement, and a food supplement.

The invention discloses a synbiotics composition, a synbiotics preparation, a preparation method and an application thereof and relates to the technical field of microorganism additives. The synbiotics preparation prepared from the synbiotics composition comprises the following components in parts by weight: 450-510 parts of rice bran, 2-8 parts of lactic acid bacteria, 1-5 parts of enterococcus faecalis, 1-5 parts of enterococcus faecium, 200-300 parts of Brady yeast, 5-12 parts of bacillus subtilis, 30-70 parts of fructo-oligose, 30-70 parts of chitosan oligosaccharide, 100-200 parts of isomalto-oligosaccharides and 0-5 parts of mildew preventives. According to the invention, the technical problems of low bacteria content, high percentage of infectious microbe and unstable quality of the presently produced synbiotics for feeding are solved. The three sugars are compounded into the prebiotics of the synbiotics composition provided by the invention, so that the prebiotics effect is enhanced, the balance of the intestinal microecology is maintained under the synergic effect of various prebiotics and synbiotics, the growth of the livestock is boosted and the intestines diseases are reduced.

The invention belongs to the technical field of microbial culture mediums, and relates to an improved bifidobacterium tomato culture medium and a preparation method thereof. According to the improvedbifidobacterium tomato culture medium, each litre of a bifidobacterium tomato culture solution consists of the following components: 9-10g of molasses, 8-9g of corn flour, 1.00-1.50ml of isoligosaccharide, 0.4-0.6g of dipotassium phosphate, 0.005-0.007g of manganese sulfate and 1L of a tomato juice basal culture medium. The improved bifidobacterium tomato culture medium provided by the invention has the advantages of being short culture duration, high in bacterium yield, good in activity, low in cost, simple in operations of the preparation method and the like.

The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the α-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of α-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.