Improved bifidobacterium tomato culture medium and preparation method thereof

A technology of tomato culture medium and bifidobacteria, which is applied in the field of improved bifidobacterium tomato culture medium and its preparation, can solve the problems of excessive intake of chemical substances, complicated preparation process, and unsuitable intake of human body, etc., and achieve tomato The effect of wide distribution, low price and low cost

Inactive Publication Date: 2018-05-29
任秀敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of BL medium is high, the preparation process is complicated, and it ...

Method used

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Effect test

Embodiment 1

[0024] The improved bifidobacterium tomato culture medium of embodiment 1 contains the following components in every liter of bifidobacterium tomato culture solution: molasses 9.16g, corn flour 8.92g, isomaltooligosaccharide 1.30ml, dipotassium hydrogen phosphate 0.5g, Manganese sulfate 0.006g, tomato juice basal medium 1L, wherein the tomato juice basal medium is mixed with pure tomato juice and water at a volume ratio of 1:4.

[0025] The preparation method of every liter of improved bifidobacterium tomato culture medium, the steps are as follows:

[0026] Step 1: Weigh 9.16g of molasses, 8.92g of corn flour, 1.30ml of isomaltooligosaccharide, 0.5g of dipotassium hydrogen phosphate, and 0.006g of manganese sulfate;

[0027] Step 2: Select fresh tomatoes, remove the damaged part, cut into pieces, mash with a multifunctional blender, mix pure tomato juice and water with a volume ratio of 1:4 to make a 1L solution, and then filter through two layers of gauze to obtain tomato b...

Embodiment 2

[0031] The improved bifidobacterium tomato medium of embodiment 2 contains the following components in every liter of bifidobacterium tomato culture solution: molasses 9.51g, corn flour 8.25g, isomaltooligosaccharide 1.10ml, dipotassium hydrogen phosphate 0.4g, Manganese sulfate 0.005g, tomato juice basal medium 1L, wherein the tomato juice basal medium is mixed with pure tomato juice and water at a volume ratio of 1:4.

[0032] The preparation method of every liter of improved bifidobacterium tomato culture medium, the steps are as follows:

[0033] Step 1: Weigh 9.51g of molasses, 8.25g of corn flour, 1.10ml of isomaltooligosaccharide, 0.4g of dipotassium hydrogen phosphate, and 0.005g of manganese sulfate;

[0034] Step 2: Select fresh tomatoes, remove the damaged part, cut into pieces, mash with a multifunctional blender, mix pure tomato juice and water with a volume ratio of 1:4 to make a 1L solution, and then filter through two layers of gauze to obtain tomato basic med...

Embodiment 3

[0038] The improved bifidobacterium tomato medium of embodiment 3 contains the following components in every liter of bifidobacterium tomato culture solution: molasses 9.95g, corn flour 8.48g, isomaltooligosaccharide 1.45ml, dipotassium hydrogen phosphate 0.6g, Manganese sulfate 0.007g, tomato juice basal medium 1L, wherein the tomato juice basal medium is mixed with pure tomato juice and water at a volume ratio of 1:4.

[0039] The preparation method of every liter of improved bifidobacterium tomato culture medium, the steps are as follows:

[0040] Step 1: Weigh 9.95g of molasses, 8.48g of corn flour, 1.45ml of isomaltooligosaccharide, 0.6g of dipotassium hydrogen phosphate, and 0.007g of manganese sulfate;

[0041] Step 2: Select fresh tomatoes, remove the damaged part, cut into pieces, mash with a multifunctional blender, mix pure tomato juice and water with a volume ratio of 1:4 to make a 1L solution, and then filter through two layers of gauze to obtain tomato basic med...

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Abstract

The invention belongs to the technical field of microbial culture mediums, and relates to an improved bifidobacterium tomato culture medium and a preparation method thereof. According to the improvedbifidobacterium tomato culture medium, each litre of a bifidobacterium tomato culture solution consists of the following components: 9-10g of molasses, 8-9g of corn flour, 1.00-1.50ml of isoligosaccharide, 0.4-0.6g of dipotassium phosphate, 0.005-0.007g of manganese sulfate and 1L of a tomato juice basal culture medium. The improved bifidobacterium tomato culture medium provided by the invention has the advantages of being short culture duration, high in bacterium yield, good in activity, low in cost, simple in operations of the preparation method and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial culture medium, and relates to an improved bifidobacterium tomato medium and a preparation method thereof. Background technique [0002] Microecological regulators are probiotics and their metabolite products that can adjust microecological disorders and improve the health of the host. Bifidobacteria are one of the main members of the normal intestinal microflora of humans and animals (including insects). Among probiotics It is the most widely used probiotic preparation at home and abroad. Bifidobacteria are probiotics colonized in the intestinal tract of healthy human beings. From birth to death, Bifidobacteria accompany people throughout their life. As an excellent ecological strain, the existence and quantity of Bifidobacteria are closely related to human health. It has physiological functions such as inhibiting the growth of intestinal pathogenic bacteria, improving protein metabolism, prov...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 任秀敏
Owner 任秀敏
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