Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

In vitro preservation method of plasmopara viticola

A technology for in vitro preservation of grape downy mildew, applied in the field of in vitro preservation of grape downy mildew, can solve the problems of inability to contact with the skin, tingling on the skin, frostbite and the like, and achieve the effect of a simple and effective preservation method

Inactive Publication Date: 2018-09-04
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dimethyl sulfoxide has a certain toxic effect. High volatile concentration may cause headache and dizziness, and it can burn the skin and make the skin feel tingling; liquid nitrogen is a gas that suffocates people, and low temperature will cause frostbite to people. Contact with the skin; so although the above protective agents are commonly used, they also have their own disadvantages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In vitro preservation method of plasmopara viticola
  • In vitro preservation method of plasmopara viticola
  • In vitro preservation method of plasmopara viticola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for in vitro preservation of downy mildew bacteria. During the disease occurrence period, collect well-grown and infected single-spotted leaves of downy mildew. Each piece is separated by newspaper and packaged separately. The samples are put into a foam box with ice packs, and the Back to the lab. Then spray the surface of the diseased leaves with a throat sprayer filled with distilled water, use sterile distilled water to wash away the sundries and the original downy mildew mold layer on the surface of the diseased leaves, wash each diseased leaf separately, and do not mix them together, and then wash the diseased leaves Put it in a porcelain plate covered with multiple layers of moist gauze or filter paper, with the back of the leaf facing up, wrap the petiole with soaked cotton, use the filter paper to dry the remaining water droplets on the surface of the diseased leaf, seal the opening of the container with plastic wrap, and cover it with black Arranged a...

Embodiment 2

[0026] A method for in vitro preservation of grape downy mildew bacteria. During the disease occurrence period, collect well-grown and infected single-spotted leaves of grape downy mildew, separate and pack each piece with newspaper, and put the samples into a foam box with ice packs , brought back to the laboratory. Then use a throat sprayer filled with distilled water to spray the surface of the diseased leaves, and use sterile distilled water to wash away the sundries on the surface of the diseased leaves and the original mold layer of grape downy mildew. Each diseased leaf is washed separately and cannot be mixed together, and then the diseased leaves Put the leaves in a container covered with multiple layers of moist gauze or filter paper, with the back of the leaf facing up, wrap the petiole with soaked cotton, use the filter paper to absorb the remaining water droplets on the surface of the diseased leaf, seal the opening of the container with plastic wrap, and cover it ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an in vitro preservation method of plasmopara viticola. According to the method, plasmopara viticola is embedded in agar powder, and then preserved at -80 DEG C; after 90 daysof preservation, healthy leaves of grapes are still pathogenic for back grafting; and a specific preservation treatment method and conditions of plasmopara viticola, and the processes of determinationof sporangium pathogenicity after preservation are also included in the method. The method provided by the invention is an ideal, simple and effective preservation method.

Description

technical field [0001] The invention relates to the field of in vitro preservation of pathogens, in particular to a method for in vitro preservation of grape downy mildew. Background technique [0002] Grape downy mildew (Plasmopara viticola (Berk. & Curtis.) Berl et de Toni) belongs to the subdivision Flagellate, Oomycetes, Peronomycetes, Peronosporaceae, and the genus Uniaxial. The pathogen is a diploid biotrophic obligate parasite. The sporangia of the asexual stage are its propagules. The sexual stage produces oospores. [0003] Because grape downy mildew is a parasitic bacterium, it cannot be cultivated and preserved on artificial medium so far, which has brought great troubles to the study of the pathogen in different periods and locations. At present, there are many reports on the in vitro preservation of obligate parasites at home and abroad. As reported by Gill and Davidson, the sporangia of vetch fungus can be stored at -80°C for one year after being treated wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12R1/645
CPCC12N1/04
Inventor 宋双姜彩鸽张怡王国珍樊仲庆张华普
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com