Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage

A non-destructive, moisture-resistant technology, applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of complex technology, shorten the breeding cycle, and high cost, and achieve good correlation, easy learning and Operation, high accuracy effect

Inactive Publication Date: 2012-07-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-destructive rapid identification of cucumber heat resistance and humidity resistance at the seedling stage can improve breeding efficiency and

Method used

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  • Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage
  • Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage
  • Method rapidly identifying cucumber breeding material with heat resistance and moisture resistance without damaging seedling stage

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Experimental program
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Embodiment 1

[0014] 2 North China open field cucumbers, 2 North China spring and autumn greenhouse type cucumbers, 2 North China and European hybrid cucumbers, 2 Thailand native type cucumbers, Thailand native type and North China type hybrid cucumber F 1 2 servings, hybrid cucumber F of Thai native type and North China type 2 2 servings, 1 European type cucumber, 1 Japanese type cucumber.

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Abstract

The invention discloses a method rapidly identifying a cucumber breeding material with heat resistance and moisture resistance without damaging a seedling stage, which comprises the following steps carried out in a manual simulation environment with daytime/night=12hr/12hr, daytime temperature of 35 DEG C, night temperature of 25 DEG C, daytime humidity of 80%, night humidity of 100% and daytime illumination intensity of 40,000lux: (1) carrying out first screening on the seventh day after cucumber sowing, and selecting seedlings with the length of hypocotyls not exceeding 4cm; (2) on the ninth day after sowing, selecting the seedlings where first main leaves have the sizes not exceeding 4*5cm and the main leaves and edges of cotyledons are free of thermal burn; and (3) respectively inoculating plasmopara viticola and zucchini yellow mosaic virus (ZYMV) on the seedlings selected in the step (2) at the period of a third main leaf. The seedlings having heat resistance and moisture resistance to the plasmopara viticola and the ZYMV are the cucumber breeding material with the heat resistance and the moisture resistance. The method rapidly identifying the cucumber breeding material with the heat resistance and the moisture resistance without damaging the seedling stage causes no damage to the seedlings, can identify the cucumber breeding material with the heat resistance and the moisture resistance through three simple morphological characteristic indexes and is learned and operated easily. Besides, the method is good in relevance and high in accuracy rate.

Description

technical field [0001] The invention relates to a method for quickly identifying cucumber breeding materials with heat and moisture resistance without damage at the seedling stage. Background technique [0002] With global warming, the average temperature in North China is gradually rising, summer is prolonged, winter is shortened, the distribution of rainwater is unbalanced, and the proportion of cucumbers in protected areas is increasing. Factor. Taking precautions, strengthening the research work on heat and humidity resistance of cucumber, and screening out a number of related resources is an important issue facing cucumber breeders at present. Rapid identification of heat and humidity resistance of cucumbers at the seedling stage can improve the breeding efficiency and shorten the breeding cycle. The previous breeding methods required a large number of physiological and biochemical analyses, which were costly and complicated in technology. SUMMARY OF THE INVENTION ...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06
Inventor 张峰刘立功
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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