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Bacillus amyloliquefaciens strain with broad-spectrum resistance to fruit tree pathogenic fungi and its application

A technology for dissolving starch spores and bacilli, applied in application, bacteria, fungicides, etc., can solve the problems of less fungal disease of fruit trees, polluted environment, and pesticide residues harming human and animal health, etc., and achieves good growth, simple culture conditions, and acid-base stability. good effect

Active Publication Date: 2018-04-10
SHANDONG YANTAI AGRI SCI & TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical fungicides are still mainly used to prevent and control fruit tree diseases. The advantages of chemical fungicides are fast action, good effect, and convenient use. They can eliminate or control the diseases that occur in a short period of time, and effectively reduce losses. It also makes mankind face new problems: a large number of pesticide residues endanger the health of humans and animals, and pollute the environment
At present, my country has successfully developed and put into production a variety of Bacillus commercial preparations, mainly to prevent fungal diseases such as sheath blight, powdery mildew, rice blast and other fungal diseases of grain and vegetables, but there are few biological preparations for fungal diseases of fruit trees

Method used

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  • Bacillus amyloliquefaciens strain with broad-spectrum resistance to fruit tree pathogenic fungi and its application
  • Bacillus amyloliquefaciens strain with broad-spectrum resistance to fruit tree pathogenic fungi and its application
  • Bacillus amyloliquefaciens strain with broad-spectrum resistance to fruit tree pathogenic fungi and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Isolation and identification of Bacillus amyloliquefaciens YTB1407

[0026] The roots of American ginseng planted in Fushan District, Yantai City, Shandong Province, China, are cultivated and identified in the laboratory according to the following conditions, and can obtain the broad-spectrum antibacterial Bacillus amyloliquefaciens YTB1407 of the present invention:

[0027] Isolation and purification of YTB1407: The roots of American ginseng were surface sterilized, cut into small pieces, and cultured on NA solid medium. After 2 days, pick a single colony, inoculate it on NA solid medium by streaking method, culture at 37°C for 1-2 days, pick a single colony and save it.

[0028] The Bacillus amyloliquefaciens YTB1407 described in the present invention is identified according to its colony morphology, individual morphological characteristics, physiological and biochemical characteristics, phylogenetic tree of 16S rDNA and gyrA gene sequence, and the like.

[0029] The...

Embodiment 2

[0035] Cultivation and fermentation conditions of the present invention

[0036] (1) Slope culture conditions: beef extract peptone solid medium: beef extract 3g, peptone 10g, NaCl 5g, agar powder 15g, water 1000mL, pH 7.0-8.0. 121°C, 0.1MPa steam sterilization for 20 minutes; culture temperature 37 ℃, culture time 2-4 days.

[0037] (2) Fermentation culture conditions: beef extract peptone liquid medium: beef extract 3g, peptone 10g, NaCl 5g, water 1000mL, pH 7.0-8.0. 121°C, 0.1MPa steam sterilization for 20 minutes; inoculate the culture with 2% amount, inoculated in the fermentation medium, the liquid content of the fermentation medium is 100mL (the container is a 250mL Erlenmeyer flask), the rotating speed is 150 rpm, the culture temperature is 30°C, and the culture time is 4 days to obtain the fermentation liquid.

[0038] The fermented liquid produced by Bacillus amyloliquefaciens YTB1407 of the present invention is centrifuged at 5000 rpm for 10 minutes, and the precip...

Embodiment 3

[0040] Analysis of the antibacterial activity of broad-spectrum antibacterial thallus and fermentation broth produced by Bacillus amyloliquefaciens YTB1407 of the present invention

[0041] The indicator bacteria (apple ringworm, apple spotted leaf rot, grape white rot and grape anthracnose) were placed in the middle of the potato solid medium plate with a diameter of 6mm, and the antagonistic bacteria YTB1407 was inoculated around by the cross method. See attached Figure 4 . The following table shows the size of the inhibition zone of Bacillus amyloliquefaciens YTB1407 to different fruit tree phytopathogenic fungi:

[0042] Table 2 The size of the inhibition zone of Bacillus amyloliquefaciens YTB1407 to different fruit tree phytopathogenic fungi

[0043] fruit tree pathogenic fungi

Inhibition zone

Apple ringworm

8.11

Apple spot deciduous

9.45

Grape white rot

8.75

Grape anthracnose

7.72

[0044] Take the aseptic fer...

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Abstract

The invention relates to an antagonistic strain which is finally identified as Bacillus amyloliquefaciens through physiological and biochemical reaction, 16SrDNA and gyrA gene sequencing and sequence alignment; the Bacillus amyloliquefaciens is named Bacillus amyloliquefaciens YTB1407. The Bacillus amyloliquefaciens strain has antagonistic activity to various fruit tree pathogenic fungi, including macrophoma kawatsukai, ahernaria mali, grape anthracnose, coniothyrium diplodiella, plasmopara viticola and the like; the Bacillus amyloliquefaciens strain is antibacterial in broad spectrum, stable in anti-microbial activity and lasting in effect and has the huge potential for developing novel, efficient and natural biological control agents.

Description

technical field [0001] The present invention relates to Bacillus amyloliquefaciens YTB1407 strain Bacillus amyloliquefaciens YTB1407, and also relates to the indoor antagonistic activity and stability analysis of the strain against various fruit tree pathogenic fungi. Background technique [0002] Fruit tree disease is an important factor affecting the growth and fruiting process of fruit trees, which seriously restricts the yield and quality of fruit, and causes huge economic losses to the world every year. At present, chemical fungicides are still mainly used to prevent and control fruit tree diseases. The advantages of chemical fungicides are fast action, good effect, and convenient use. They can eliminate or control the diseases that occur in a short period of time, and effectively reduce losses. Human beings are faced with new problems: a large amount of pesticide residues endanger human and animal health and pollute the environment. Therefore, the development and util...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 王英姿于晓丽李宝燕王培松
Owner SHANDONG YANTAI AGRI SCI & TECH INST
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