Application of Streptomyces corchorusii NF0919 strain metabolite in grape disease control
A technology for Streptomyces jute, metabolites, applied in the directions of applications, microorganism-based methods, chemicals for biological control, etc., can solve the problems of unmentioned and disclosed
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[0020] For the strain characteristics of the Streptomyces jute NF0919 strain provided in the present invention, see patent ZL201010236886.1. The extraction method of product in Streptomyces jute NF0919 strain fermented liquid of the present invention comprises the following steps:
[0021] (A) Activation of the strain and cultivation of primary seed culture medium
[0022] The slant of the preserved NF0919 strain was activated on Gao’s No. 1 medium, cultured at 30°C for 10 days, and 1 cm 2 Inoculate 50 mL of primary seed culture solution (50 mL of culture solution in a 250 mL Erlenmeyer bottle) with a large or small bacterial block, place on a shaker at 170 rpm at 30°C for 44-48 h, and the 50 mL of primary seed culture The composition of the liquid is: 3% soluble starch, 2% glucose, 1% yeast powder, 1% peptone, 0.5% NaCl, K 2 HPO 4 0.2%, soluble starch, glucose, yeast powder, peptone, NaCl and K 2 HPO 4 The unit ratio of content is W / V.
[0023] (B) Cultivation of se...
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- IPC
- C12P1/04; A01N63/02; A01P3/00; C12R1/465
- Inventors
- 杨敬辉; 陈宏州