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Application of Streptomyces corchorusii NF0919 strain metabolite in grape disease control

A technology for Streptomyces jute, metabolites, applied in the directions of applications, microorganism-based methods, chemicals for biological control, etc., can solve the problems of unmentioned and disclosed

Inactive Publication Date: 2013-01-30
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most bacterial strains or preparations can only prevent and treat one kind of disease, but it is not mentioned and disclosed in the prior art to use the same antagonistic actinomycete to simultaneously treat multiple diseases of grapes

Method used

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  • Application of Streptomyces corchorusii NF0919 strain metabolite in grape disease control
  • Application of Streptomyces corchorusii NF0919 strain metabolite in grape disease control

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Embodiment

[0020] For the strain characteristics of the Streptomyces jute NF0919 strain provided in the present invention, see patent ZL201010236886.1. The extraction method of product in Streptomyces jute NF0919 strain fermented liquid of the present invention comprises the following steps:

[0021] (A) Activation of the strain and cultivation of primary seed culture medium

[0022] The slant of the preserved NF0919 strain was activated on Gao’s No. 1 medium, cultured at 30°C for 10 days, and 1 cm 2 Inoculate 50 mL of primary seed culture solution (50 mL of culture solution in a 250 mL Erlenmeyer bottle) with a large or small bacterial block, place on a shaker at 170 rpm at 30°C for 44-48 h, and the 50 mL of primary seed culture The composition of the liquid is: 3% soluble starch, 2% glucose, 1% yeast powder, 1% peptone, 0.5% NaCl, K 2 HPO 4 0.2%, soluble starch, glucose, yeast powder, peptone, NaCl and K 2 HPO 4 The unit ratio of content is W / V.

[0023] (B) Cultivation of se...

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Abstract

The invention relates to a method for extraction of an active metabolite from a Streptomyces corchorusii NF0919 strain (with a preservation number of CGMCC No.3968 in CGMCC and a strain patent number of ZL201010236886.1) fermentation broth. The metabolite can effectively control Grape Anthracnose caused by plant pathogenic fungus Glomerella cingulata infection, Caucasus grape black rot caused by Physalospora baccae infection, Grape Alternaria Rot caused by Alternaria viticola infection, Grape white rot caused by Coniothyrium dlodiella infection, Grape Downy mildew caused by Plasmopara viticola infection, grape grey mould caused by Botrytis cinerea infection, grape spot anthracnose caused by Elsinae ampelina infection, and other plant diseases.

Description

field of invention [0001] The present invention relates to a kind of microorganism and application, more specifically relate to a kind of Streptomyces jute ( Streptomyces corchorusii ) The extraction method of metabolites in the fermentation liquid of NF0919 strain, and the application of the extract in the prevention and control of grape diseases belong to the technical field of biological pesticides. Background technique [0002] By the phytopathogenic fungus Clusaria pericytes ( Glomerella cingulata ) Infection caused by grape anthracnose, cystosporum ( Physalospora baccae ) infection caused by grape house blight, Alternaria ( Alternaria viticola ) caused grape cob brown rot (blight) disease, Coniothyrium dlodiella Grape white rot caused by infestation, Plasmodium ( Plasmopara viticola ) infection caused by grape downy mildew, Botrytis cinerea ( Botrytis cinerea ) infection caused by botrytis cinerea and scabella ( Elsinae ampelina ) infection caused by grape...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04A01N63/02A01P3/00C12R1/465
Inventor 杨敬辉陈宏州庄义庆
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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