7 results about "Alternaria brassicae" patented technology

The application discloses a kind of steroidal compounds with anti-parkinson disease activity and its preparation method and application.The steroidal compound has the structure as shown in formula (I) or formula (II), or stereoisomer, tautomer, pharmaceutically acceptable salt or prodrug of the structure shown in formula (I) or formula (II).The application is fermented by fungal co-cultivation to alternaria brassicae and penicillium granatum, and the expression of silent gene of strain is promoted by adopting co-cultivation and optimizing medium condition strategy, novel secondary metabolite is obtained, and new steroidal compound with treatment parkinson disease value and application prospect is extracted and separated from co-cultivation ferment, which provides great prospect for the research and development of parkinson disease treatment drugs.

The invention discloses an InDel molecular marker for detecting black spot resistance of broccoli, a method and application, and relates to the field of molecular biology. The site is located at 56724071bp-56724089bp of a broccoli chromosome 5, and the site has insertion / deletion variation of 18bp basic groups (the nucleotide sequence is as shown in SEQ ID NO. 1). When the site has a 18bp basic group (the sequence of an amplification product is shown as SEQ ID NO.2), the broccoli is a black spot resistant plant; otherwise, the plants are black spot sensitive plants. The invention also discloses a pair of specific primers for detecting the InDel molecular marker, and the nucleotide sequences of the specific primers are respectively shown as SEQ ID NO. 4 and SEQ ID NO. 5. The InDel molecular marker can rapidly and accurately detect the black spot resistance of broccoli germplasm resources at low cost, does not need field inoculation identification, can be used for early screening of broccoli black spot resistance varieties and molecular marker-assisted breeding, significantly improves the breeding efficiency, and shortens the breeding period.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The present invention provides a method for cultivating symbiotic bacteria that promote the growth of Pseudomonas aeruginosa and its application. The cultivation method comprises the following steps: (1) sterilizing the symbiotic bacteria culture medium at 120-125° C. for 10-20 minutes; (2) adding Alternaria alternata BB-1, Alternaria brassicae BB-6, and Alternaria albus BB-8 to the symbiotic bacteria culture medium respectively; maintaining the temperature of the symbiotic bacteria culture medium at 29-30° C., first aerobically fermenting and culturing for 6-7 hours, then anaerobic fermenting and culturing for 3-4 hours, and repeating this process for 50-80 hours to obtain a symbiotic culture product. The present invention uses DSEs of Alternaria alternata BB-1, Alternaria brassicae BB-6, and Alternaria albus BB-8 to establish a mutualistic symbiotic relationship with the host plant Pseudomonas aeruginosa in a long-term ecological adaptation process.

The invention belongs to the technical field of agricultural biological control, and particularly relates to bacillus velezensis, fermentation liquor, a biocontrol microbial inoculum and application of the bacillus velezensis, the fermentation liquor and the biocontrol microbial inoculum. The bacillus velezensis is separated from the Tibet Plateau environment, the preservation number is CGMCC No.37159, and the bacillus velezensis has the advantages of low temperature resistance, high temperature resistance, broad-spectrum plant disease control capacity, high antibacterial capacity and crop growth promotion. The strain can inhibit growth of pathogenic bacteria such as botrytis cinerea, fusarium graminearum, verticillium dahliae, aspergillus niger, sclerotinia sclerotiorum, alternaria brassicae and the like, and has a remarkable prevention and control effect on pathogenic bacteria-alternaria of tomato early blight in plateau areas; the strain and a fermentation liquid or a biocontrol inoculant prepared from the strain have a good application prospect in the aspect of agricultural biological control.

The application belongs to the field of agricultural microorganism technology, and discloses bacillus velezensis Bac8 and application thereof in prevention and treatment of black spot disease of cabbage. The strain is isolated from soil of a cabbage planting field, and has been preserved in China Center for Type Culture Collection on February 28, 2026, with a preservation number of CCTCC NO: M 2026354. The strain has significant antagonistic effect on alternaria brassicae, and can be used for development of a biocontrol product, and has a good application prospect in prevention and treatment of black spot disease of cabbage caused by alternaria brassicae.

The application discloses an indoor high-efficiency identification method of Chinese cabbage black spot induced by Leptosphaeria maculans, and comprises the following steps: S1, Chinese cabbage seedling culture; S2, preparation of spore suspension; S3, inoculation of test materials; and S4, culture and investigation of the test materials. Compared with the prior art, the application has the advantages of significantly shortened identification period, realized high-efficiency screening, high controllability of environment, high repeatability of results, reduced risk of misjudgment, saved resource cost and consistent green and sustainable agricultural development direction.