31 results about "Alternaria brassicae" patented technology

The invention relates to bacillus subtillis R31 with broad-spectrum antagonism capacity on plant pathogenic fungi, and in particular provides a bacterial strain (CCTCC NO: M209261) of the Bacillus subtillis R31 as well as growth inhibition action thereof on the plant pathogenic fungi and especially capacity thereof on the biological prevention and control of banana vasicular wilt. The plant endophytic bacillus subtillis is proved to be new bacillus subtillis through obtained 16S rDNA and gyrA and gyrB gene segments as well as BLAST search and comparison on NCBI (National Center of Biotechnology Information) and has better flat antagonism activity on multiple plant pathogenic fungi, better antagonism capacity especially on various soil-borne and vascular bundle diseases, i.e. fusarium oxysporum, rhizoctonia solani, alternaria, and the like, and better prevention and control effect in greenhouses and fields.

The invention relates to a bacillus subtilis str.TR21 (hereinafter referred to as 'TR21') strain having broad-spectrum antagonism to plant pathogenic fungi, in particular to the bacillus subtilis TR21 strain and a growth inhibition function thereof on the plant pathogenic fungi. The bacillus subtilis TR21 strain provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) in January 20, 2010 with a preservation number of CCTCC No: M2010019, wherein the China Center for Type Culture Collection is located in Wuhan University, Lojia Mountain, Wuchang, Hubei, China. The strain provided by the invention is proved by obtained gene segments 16S rDNA, gyrA and gyrB and BLAST search and comparison on NCBI to be a novel bacillus subtilis strain having relatively higher flat antagonistic activity to a plurality of plant pathogenic fungi, particularly having high antagonism to various soil borne vascular diseases, such as fusarium oxysporum, rhizoctonia solani, alternaria and the like, and having good prevention and control effects shown in fields.

The present invention relates to compositions containing the fungus Alternaria alternata and, or the secondary metabolites thereof, belonging to the family of the diketopiperazines (DKPs), for controlling the pathogen fungus Plasmopara viticola.

The invention relates to a streptomyces viridocyaneus Sv-29 and an application thereof, and belongs to the field of biotechnology. The streptomyces viridocyaneus (S. viridocyaneus) Sv-29 is obtained from nature by a general method. The S. viridocyaneus Sv-29 is preserved in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC NO.7398. The S. viridocyaneus Sv-29 can produce various antibacterial active substances, and has good antagonism against various fungal pathogens. Especially, the S. viridocyaneus Sv-29 has stronger prevention and treatment effects on Chinese cabbage alternaria leaf spot caused by alternaria brassicae and cotton fusarium wilt caused by fusarium oxysporum f. sp. vasinfectum.

The invention discloses a bacillus subtilis KN-104 strain. The strain is deposited in China Center for Type Culture Collection with the deposit number of CCTCC NO: M2017697. By directional target screening, the obtained bacillus subtilis is high in specificity of disease resistance, has a remarkable prevention effect on rice blast, leaf blast, rice sheath blight and alternaria brassicae, but doesnot have prevention effect on diseases such as botrytis cinerea, watermelon wilt disease, apple anthracnose, soybean root rot and tomato leaf mould. Meanwhile, the strain is high in fertility and doesnot have hemolysis.

The invention relates to the field of bacillus, in particular to a bacillus subtilis strain NH-6 and application thereof. The bacillus subtilis strain NH-6 is deposited at China General Microbiological Culture Collection Center with the preservation number of CGMCC 17815. The bacillus subtilis strain NH-6 has the advantages such as simple culture method, high growth rate, wide antibacterial spectrum and high resistance to stress. A biological control preparation prepared by the bacillus subtilis strain NH-6 can be used as a biological pesticide or a biological fertilizer to prevent and controlroot-knot nematode diseases and various plant pathogenic fungi including alternaria brassicae, banded sclerotial blight, botrytis cinerea and the like.

The invention discloses a biopesticide without pesticide residue; the biopesticide comprises the materials in parts by weight: 35-46 parts of alternaria tenuis nees, 20-40 parts of jasmine oil, 12-18 parts of rice hull powder, 21-26 parts of amino acid, 5-12 parts of allicin, 5.5-7 parts of magnesium phosphate, 5-8 parts of anise, 35-46 parts of corn straws, 10-16 parts of verticicladiella powder, 5-19 parts of amino-oligosaccharin, 25-40 parts of erythrina indica sawdust and 80-100 parts of water. The biopesticide has no pollution to the environment, safe to humans and domestic animals and low in price, has no pesticide residue and is a nuisanceless biopesticide.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker which is used for identifying alternaria brassicae of a sand pear variety, and belongs to the technical field of molecular genetic breeding of fruit trees. A method for identifying the alternaria brassicae of the sand pear variety comprises the following steps: (1) extracting a genomic DNA (Deoxyribonucleic Acid) of a to-be-detected pear; (2) carrying out a PCR (Polymerase Chain Reaction) amplification reaction by taking the genomic DNA of the to-be-detected pear as a template and utilizing a primer pair; (3) detecting a PCR amplification product, wherein through sequence alignment, basic group in an S83.0_275630 position is the SNP marker of a high-susceptible variety or a high-resistance variety for identifying the alternaria brassicae of the sand pear variety, and the primer pair comprises a forward primer S83-F, SEQ ID NO.1 and a reverse primer S83-R, SEQ ID NO.2. According to the SNP molecular marker, which is screened by using the method and is used for identifying the resistance to the alternaria brassicae of the sand pear variety, disclosed by the invention, a theoretical foundation is laid for the research on molecular breeding of resistance to the alternaria brassicae and a mechanism of resistance to the alternaria brassicae of sand pears.

The invention discloses a strain of bacillus subtilis Y2 and application thereof to prevention and treatment of the Korla pear blackhead disease. The Y2 strain is collected in China Center for Type Culture Collection in Wuhan University in China on October 30, 2018, and the collection number is CCTCC NO: M2018678. In plate antagonistic experiments, it is found that the Y2 strain has the activity of inhibiting the main pathogenic bacteria alternaria brassicae XL2 of the Korla pear blackhead disease, and the hypha growth inhibition rate reaches 42.74%; and a sterile filtrate of the Y2 strain also has very high bacteriostatic activity. Inoculation experiments on Korla pears prove that the Y2 strain can inhibit infection and colonization of pathogenic bacteria of the Korla pear blackhead disease. Therefore, the bacillus subtilis Y2 can be applied to prevention and treatment of the Korla pear blackhead disease as a biocontrol bacterium.

The invention discloses a botanical biopesticide which comprises the following substances in parts by weight: 15-27 parts of KPT EC (emulsifiable concentrate), 3.5-6.5 parts of monoammonium phosphate, 12-18 parts of carclazyte, 3-6 parts of furfuran resin, 11-19 parts of queretagetin, 5-10 parts of glyphosate isopropylamine, 15-25 parts of alternaria tenuis, 15-30 parts of oleic acid, 5-17 parts of vaseline, 3.5-6 parts of sodium sorbate, 20-30 parts of isopropanol, 5-7 parts of gastrodia elata, 11-18 parts of ricinoleate polymer, and 45-62 parts of eugenol. The botanical biopesticide can prevent and control various crop diseases, reduces the dose of pesticides, lowers the pesticide use cost of farmers, adopts a pure plant solvent, is low-carbon and environmental-friendly, reduces the pollution and hazard to the environment, and is safe and environmental-friendly to use.

The invention discloses a biological pesticide capable of controlling a pest source. The biological pesticide comprises the following components in parts by weight: 9-15 parts of beauveria bassiana, 34-44 parts of matrine, 12-32 parts of altenusin, 10-20 parts of sunflower seed oil, 55-69 parts of tea saponin, 1-3 parts of sodium methylene bis-naphthalene sulfonate, 5-8 parts of benzoyl peroxide, 5-7 parts of avermectin, 3-4 parts of methacrylic acid, 11-17 parts of potassium polymetaphosphate and 20-30 parts of silicone oil. Due to use of natural plant extracts, the biological pesticide provided by the invention can be used for inhibiting the growth and reproduction of pests and diseases, and the sterilization efficiency is high; except for crops, the pesticide is effective against common aphids and red spiders of flowers and the like, so that the pests are eliminated from the source.

The present invention relates to a strain of Bacillus subtilis R31 with broad-spectrum antagonistic ability to phytopathogenic fungi, in particular to provide Bacillus subtilis R31 (Bacillus subtilis R31) bacterial strain (CCTCC NO: M 209261) and its growth to phytopathogenic fungi Inhibition, especially in the biological control of banana wilt. The plant endophytic Bacillus subtilis provided by the present invention is proved to be a new Bacillus subtilis through the obtained 16S rDNA, gyrA and gyrB gene fragments, and through BLAST search and comparison on NCBI, capable of treating various plant pathogens The fungus has good plate antagonistic activity, especially for various soil-borne vascular diseases such as Fusarium oxysporum, Rhizoctonia solani, Alternaria and other soil-borne vascular diseases, and has shown good activity in greenhouses and fields. Control effect.

The invention relates to the technical field of biology, in particular to a brassica alboglabra bailey BoWRKY33 gene and an application thereof to resistance to black spot caused by Alternaria brassicae. Specifically, the invention discloses the gene BoWRKY33 for improving the resistance of brassica alboglabra bailey to alternaria alternata and a protein coded by the gene, and further discloses agene overexpression vector 35SPro: BoWRKY33-YFP and a gene silencing VIGS vector BoWRKY33-PTY.

The invention discloses application of a dehydroabietylamine (substituted) benzaldehyde Schiff base derivative, which is used for wood preservation. The dehydroabietylamine (substituted) benzaldehydeSchiff base derivative disclosed by the invention has an effective inhibition effect on botrytis cinerea, fusarium solani, fusarium graminearum, fusarium oxysporum, alternaria brassicae, coriolus versicolor and the like, and can be directly used for wood preservation or preparation of wood preservatives; and the raw materials are wide in sources and low in cost, and the dehydroabietylamine (substituted) benzaldehyde Schiff base derivative has the advantages of naturalness, no toxicity, renewability and the like.

The invention relates to a red date alternaria brassicae occurrence rule. A pesticide which can effectively control the red date alternaria brassicae is screened out, and the mix proportion of the pesticide is determined. Eleven kinds of bactericides which are common in Alaer and Aksu agricultural commodities markets are screened out. The screening result indicates that pyraclostrobin tops the virulence for spores of alternaria brassicae, followed by fluorosilicone, difenoconazole and carbendazim. When carbendazim and pyraclostrobin are combined in the ratio of 1:6, the mixture reaches the highest virulence and has an obvious synergistic effect.

The invention relates to a fragrance generating fungus and application thereof, and belongs to the field of microorganism. The fragrance generating fungus is common tubercularia vulgaris GS-1, the collection organization is the China Center for Type Culture Collection, the collection date is July 4, 2016, and the collection number is CCTCC NO. M 2016369. The fragrance generating fungus can be used for preventing and controlling botrytis cinerea, bipolaris oryzae, fusarium graminearum, alternaria brassicae, sclerotinia sclerotiorum and fruit botrytis cinerea, so that the preventing and controlling research thought on various plant diseases can be broadened, and the limitation on development and utilization of antibiological inoculants in China can be solved within a certain range.

The invention relates to application of a 1-sulfonyl naphthol derivative in prevention and treatment of plant pathogenic fungi and an antibacterial agent, and belongs to the technical field of antibacterial agents. In the application of the 1-sulfonyl naphthol derivative, the 1-sulfonyl naphthol derivative has a structure shown as a formula I, and in the formula I, R is C1-C6 alkyl, aryl or heteroaryl, wherein the aryl is phenyl, substituent substituted phenyl or naphthyl. In the application of the 1-sulfonyl naphthol derivative of the present invention, the 1-sulfonyl naphthol derivative canbe used for detecting 16 plant pathogenic fungi such as fusarium graminearum, wheat root rot fungi, wheat stem rot fungi, magnaporthe oryzae, setosphaeria turcica, bipolaris maydis, maize curvularia leaf spot, oilseed rape sclerotinia sclerotiorum, tobacco fusarium oxysporum, phytophthora nicotianae, alternaria alternata, cotton fusarium oxysporum, cucumber fusarium oxysporum and botrytis cina, alternaria brassicae and phytophthora capsici, and shows good antibacterial activity.

The invention provides plant pesticide and a preparation method and application thereof. The plant pesticide is prepared from aralia chinensis and pterocarya stenoptera. The preparation method includes: putting raw materials into a reaction kettle, and injecting water according to a solid-liquid weight ratio of 1:4; using an electric heating steam generator to heat for 4h to 80 DEG C, and enteringa centrifugal machine for separation of liquid and residue; concentrating the liquid to obtain the plant pesticide. The plant pesticide has the advantages that the plant pesticide can effectively inhibit common fruit-vegetable pest like cabbage caterpillar, aphid, plutella xylostella, prodenia litura, red spider and empoasca flavescens, and one-time killing rate is up to higher than 80%; the plant pesticide has certain prevention and control effect on the common fruit-vegetable pest like alternaria brassicae.

The invention relates to a bacillus subtilis str.TR21 (hereinafter referred to as 'TR21') strain having broad-spectrum antagonism to plant pathogenic fungi, in particular to the bacillus subtilis TR21 strain and a growth inhibition function thereof on the plant pathogenic fungi. The bacillus subtilis TR21 strain provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) in January 20, 2010 with a preservation number of CCTCC No: M2010019, wherein the China Center for Type Culture Collection is located in Wuhan University, Lojia Mountain, Wuchang, Hubei, China. The strain provided by the invention is proved by obtained gene segments 16S rDNA, gyrA and gyrB and BLAST search and comparison on NCBI to be a novel bacillus subtilis strain having relatively higher flat antagonistic activity to a plurality of plant pathogenic fungi, particularly having high antagonism to various soil borne vascular diseases, such as fusarium oxysporum, rhizoctonia solani, alternaria and the like, and having good prevention and control effects shown in fields.

The invention discloses a primer and a method for detecting Alternaria brassicae by LAMP, belonging to the technical field of molecular biology. A method for detecting Alternaria brassicae by LAMP disclosed in the invention comprises the steps of extracting Alternaria brassicae genomic DNA; using the genomic DNA as a template, and using primers to perform LAMP amplification; LAMP product detection: after the reaction, Directly observe the color change of the reaction solution; or detect it by agarose gel electrophoresis. A primer and a method for detecting Alternaria brassicae disclosed by the invention have strong specificity and high sensitivity; the equipment used is simple, easy to operate and easy to identify; and it can realize rapid and efficient amplification.

The invention belongs to the technical field of pesticides, and particularly relates to a phthalide hydrazide compound as well as a preparation method and application thereof. The phthalide hydrazide compound prepared by the invention has good bacteriostatic activity, and when the phthalide hydrazide compound is used as an active component in preparation of a bactericide, plant diseases caused by plant pathogenic fungi can be prevented and treated; the bactericidal composition is used for preventing and treating plant diseases caused by alternaria alternata, pyricularia grisea, phytophthora capsici, watermelon fusarium wilt, potato dry rot, sclerotinia sclerotiorum, valsa mali, botrytis cinerea, alternaria brassicae and fusarium graminearum.

The invention discloses Fenghuang Dancong tea plant Bacillus sp. ZF04 and its use in biocontrol. Through Fenghuang Dancong tea plant root sampling, separation purification, screening and culture, Bacillus sp. ZF04 GDMCC No. 60014 with good biocontrol effects is obtained. Physiological and biochemical characteristics and a molecular level of the Bacillus sp. ZF04 are obviously different from those of the common bacillus and is a typical novel strain. The Bacillus sp. ZF04 has good bacteriostasis effects on pear alternaria brassicae, walnut leaf blight pathogens, red date alternaria brassicae and rhizoctonia solani, has mycelia growth inhibition rates of 77.55%, 83.30%, 74.52% and 75.19%, has a fruit pythium hyphae growth inhibition rate of 31.60%, is typical, outstanding and specific biocontrol bacteria and has wide application values in biocontrol.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker which is used for identifying alternaria brassicae of a sand pear variety, and belongs to the technical field of molecular genetic breeding of fruit trees. A method for identifying the alternaria brassicae of the sand pear variety comprises the following steps: (1) extracting a genomic DNA (Deoxyribonucleic Acid) of a to-be-detected pear; (2) carrying out a PCR (Polymerase Chain Reaction) amplification reaction by taking the genomic DNA of the to-be-detected pear as a template and utilizing a primer pair; (3) detecting a PCR amplification product, wherein through sequence alignment, basic group in an S83.0_275630 position is the SNP marker of a high-susceptible variety or a high-resistance variety for identifying the alternaria brassicae of the sand pear variety, and the primer pair comprises a forward primer S83-F, SEQ ID NO.1 and a reverse primer S83-R, SEQ ID NO.2. According to the SNP molecular marker, which is screened by using the method and is used for identifying the resistance to the alternaria brassicae of the sand pear variety, disclosed by the invention, a theoretical foundation is laid for the research on molecular breeding of resistance to the alternaria brassicae and a mechanism of resistance to the alternaria brassicae of sand pears.

The invention discloses a bactericidal pesticide. The bactericidal pesticide is prepared from raw materials in parts by weight as follows: 12-14 parts of fentoxin, 21-23 parts of jasmine oil, 20-22 parts of rice hull powder, 10-15 parts of amino acid, 13-15 parts of allicin, 12-14 parts of magnesium phosphate, 20-25 parts of star anise, 20-24 parts of corn straw, 2-4 parts of tortoiseshell, 10-12 parts of bacillus papillae powder and 11-15 parts of herba taraxaci. The bactericidal pesticide is a novel pesticide which is efficient, targeted, environmentally friendly, free of secondary toxicity, residues, pollution or hazards and capable of keeping ecological balance.

The invention discloses a compound with anti-neurodegenerative change activity and a preparation method and application thereof. The compound with anti-neurodegenerative change activity can be produced by adopting a co-culture mode of two strains of alternaria brassicae and penicillium, and the capacity of microorganisms for synthesizing secondary metabolites is excavated as much as possible by virtue of research methods of changing a culture medium, culture conditions, a mixed culture ratio and the like. And scale-up culture can be carried out on the basis of repeatable and sustainable development of the process. The compound has neuroprotective activity on neurodegenerative change disease cells, and shows a better protective effect than a positive drug tert-butylhydroquinone (TBHQ).

The invention discloses an application of asarum caulescens and extract thereof in preventing and treating plant pathogenic fungi. The application comprises the following steps: soaking the asarum caulescens with methanol, extracting the asarum caulescens methanol extract with petroleum ether and ethyl acetate, and detecting the bacteriostatic activity of each phase extract of the asarum caulescens . Research finds that the asarum caulescens methanol extract has an obvious inhibiting effect on mycelial growth of 12 plant pathogenic fungi including pyricularia oryzae, hylocereus undatus alternaria brassicae,Colletotrichum musae, mango colletotrichum gloeosporioides, wheat scab, Macrophoma kawatsukai, Alternaria solani, Phomopsis mangiferae, and Fusarium oxysporum. Therefore, the asarum caulescens methanol extract has a certain broad-spectrum inhibition effect on pathogenic fungi, and has the potential of being developed into a botanical bactericide.

The invention relates to alternaria brassicae and application thereof, and belongs to the technical field of microorganisms. According to the invention, the alternaria brassicae is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 15, 2021, and the preservation number of the alternaria brassicae is CGMCC No.23045; the liquid culture of the alternaria brassicae strain has a remarkable inhibition effect on three pathogenic fungi including fusarium graminearum, exserohilum turcicum and brown blotch of citrus grandis, and has a wide application prospect.