13 results about "Alternaria brassicae" patented technology

The invention relates to the technical field of microorganisms, and discloses trichoderma yunnanense and application thereof. The Trichoderma yunnanense CCTCC NO: M 2025645 provided by the invention is a dominant strain of a high-yield siderophore, the activity of the siderophore is 69.79%, and the Trichoderma yunnanense can be used for synthesizing and secreting carboxylic acid type siderophore; the strain has a sulfur oxidation function; in addition, the invention also provides an application of the trichoderma sp. In regulation and control of plant diseases, the trichoderma sp. Disclosed by the invention can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and the trichoderma sp. Can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae (Fr.) Kissler, and can be used for antagonizing fusarium solani, fusarium graminearum, fusarium moniliforme, Rosellinia necatrix and Alternaria brassicae. The Trichoderma yunnanense provided by the invention is a strain separated from tobacco planting soil, and compared with a strain subjected to mutagenesis screening in an extreme environment and a laboratory, the Trichoderma yunnanense has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the Trichoderma yunnanense stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect.

The invention discloses a tea saponin bactericide. The active components in the bactericide comprise tea saponin, a microbial complex microbial inoculant and an auxiliary agent. By adding the paenibacillus, the bacillus amyloliquefaciens, the gluconacetobacter xylinum and the tea saponin into the bactericide, the activity of the navel orange colletotrichum gloeosporioides and the navel orange alternaria brassicae can be effectively inhibited.

The present invention discloses the application of isoquinoline-3-carboxylic acid in preventing and controlling plant bacterial diseases. The isoquinoline-3-carboxylic acid provided by the present invention has an obvious inhibitory effect on plant bacterial diseases, and the plant bacterial diseases are Ralstonia solanacearum, Acidovorax avenae subsp. Citrulli, Pseudomonas syringae pv. actinidiae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Alternaria brassicae, Xanthomonas citri subsp. citri, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas fragariae, Pseudomonas syringae pv. lachrymans, Dickeya dadantii, Xanthomonas axonopodis pv. mangiferaeindicae. The isoquinoline-3-carboxylic acid of the present invention has protective and therapeutic effects on Acidovorax avenae subsp. Citrulli.

The application discloses a kind of steroidal compounds with anti-parkinson disease activity and its preparation method and application.The steroidal compound has the structure as shown in formula (I) or formula (II), or stereoisomer, tautomer, pharmaceutically acceptable salt or prodrug of the structure shown in formula (I) or formula (II).The application is fermented by fungal co-cultivation to alternaria brassicae and penicillium granatum, and the expression of silent gene of strain is promoted by adopting co-cultivation and optimizing medium condition strategy, novel secondary metabolite is obtained, and new steroidal compound with treatment parkinson disease value and application prospect is extracted and separated from co-cultivation ferment, which provides great prospect for the research and development of parkinson disease treatment drugs.

The invention discloses an InDel molecular marker for detecting black spot resistance of broccoli, a method and application, and relates to the field of molecular biology. The site is located at 56724071bp-56724089bp of a broccoli chromosome 5, and the site has insertion / deletion variation of 18bp basic groups (the nucleotide sequence is as shown in SEQ ID NO. 1). When the site has a 18bp basic group (the sequence of an amplification product is shown as SEQ ID NO.2), the broccoli is a black spot resistant plant; otherwise, the plants are black spot sensitive plants. The invention also discloses a pair of specific primers for detecting the InDel molecular marker, and the nucleotide sequences of the specific primers are respectively shown as SEQ ID NO. 4 and SEQ ID NO. 5. The InDel molecular marker can rapidly and accurately detect the black spot resistance of broccoli germplasm resources at low cost, does not need field inoculation identification, can be used for early screening of broccoli black spot resistance varieties and molecular marker-assisted breeding, significantly improves the breeding efficiency, and shortens the breeding period.

The invention provides an RPA (recombinase polymerase amplification) primer group, a kit and a method for detecting the alternaria brassicae of broccoli, which are used for detecting the alternaria brassicae of broccoli. The primer group comprises specific primers F1 / R1, F1 / R1 and F2 / R1, and the sizes of theoretical amplification products of the primer group are 204bp, 187bp and 189bp. Specific target genes are screened out through comparative genomics, RPA primers are designed, and a constant-temperature (39 DEG C) and rapid (20 minutes) detection method is established. The primer provided by the invention has strong specificity, is only effective to the brassica oleracea var. Brassicae, and has no cross reaction with sibling species; the sensitivity is high, the detection lower limit is 0.08 ng / mu L, and the sensitivity is 100 times higher than that of conventional PCR. The actual sample verifies that the detection accuracy reaches 100%. The method is easy and convenient to operate, rapid in detection and suitable for field early diagnosis, plant quarantine and disease monitoring of the pathogenic bacteria.

The present invention provides a method for cultivating symbiotic bacteria that promote the growth of Pseudomonas aeruginosa and its application. The cultivation method comprises the following steps: (1) sterilizing the symbiotic bacteria culture medium at 120-125° C. for 10-20 minutes; (2) adding Alternaria alternata BB-1, Alternaria brassicae BB-6, and Alternaria albus BB-8 to the symbiotic bacteria culture medium respectively; maintaining the temperature of the symbiotic bacteria culture medium at 29-30° C., first aerobically fermenting and culturing for 6-7 hours, then anaerobic fermenting and culturing for 3-4 hours, and repeating this process for 50-80 hours to obtain a symbiotic culture product. The present invention uses DSEs of Alternaria alternata BB-1, Alternaria brassicae BB-6, and Alternaria albus BB-8 to establish a mutualistic symbiotic relationship with the host plant Pseudomonas aeruginosa in a long-term ecological adaptation process.

The invention provides a culture method of symbiotic bacteria for promoting growth of Arabidopsis arundinacea and application of the symbiotic bacteria. The culture method comprises the following steps: (1) sterilizing a symbiotic bacteria culture medium at 120-125 DEG C for 10-20 minutes; (2) respectively adding the alternaria alternata BB-1, the alternaria brassicae BB-6 and the alternaria hexaphoria BB-8 into the symbiotic bacterium culture medium; and keeping the temperature of the symbiotic bacterium culture medium at 29-30 DEG C, firstly carrying out aerobic fermentation culture for 6-7 hours, then carrying out anaerobic fermentation culture for 3-4 hours, and repeating the steps for 50-80 hours to obtain a symbiotic culture product. According to the invention, DSE of alternaria alternata BB-1, DSE of alternaria brassicae BB-6 and DSE of alternaria hexangularis BB-8 are used to establish a reciprocal symbiotic relationship with host plant Arabidopsis arundinacea in a long-term ecological adaptation process.

The present invention relates to the technical field of strain cultivation, and specifically discloses a method for cultivating a plant endophytic fungal strain, wherein the method for cultivating the plant endophytic fungal strain is a method for cultivating Alternaria brassicae BB‑6, and the deposit number of Alternaria brassicae BB‑6 is: CCTCCNO: M20232214; the specific cultivation method comprises the following steps: Alternaria brassicae is first placed in a chitosan regulating liquid for uniform dispersion treatment according to a weight ratio of 2:7, and uniform dispersion is completed to obtain a chitosan-regulated Alternaria brassicae agent. In the cultivation of the plant endophytic fungal strain of the present invention, Alternaria brassicae BB‑6 is uniformly dispersed in a chitosan regulating liquid, then cultivated with a culture modifier, and then cultivated by a coordinated cultivation process of the two, and then an antibacterial agent is formed by an acetone solvent to inhibit Aspergillus flavus.

The invention belongs to the technical field of agricultural biological control, and particularly relates to bacillus velezensis, fermentation liquor, a biocontrol microbial inoculum and application of the bacillus velezensis, the fermentation liquor and the biocontrol microbial inoculum. The bacillus velezensis is separated from the Tibet Plateau environment, the preservation number is CGMCC No.37159, and the bacillus velezensis has the advantages of low temperature resistance, high temperature resistance, broad-spectrum plant disease control capacity, high antibacterial capacity and crop growth promotion. The strain can inhibit growth of pathogenic bacteria such as botrytis cinerea, fusarium graminearum, verticillium dahliae, aspergillus niger, sclerotinia sclerotiorum, alternaria brassicae and the like, and has a remarkable prevention and control effect on pathogenic bacteria-alternaria of tomato early blight in plateau areas; the strain and a fermentation liquid or a biocontrol inoculant prepared from the strain have a good application prospect in the aspect of agricultural biological control.

The present invention relates to the field of microbial technology, and discloses a strain of Trichoderma yunnanensis and its application. Trichoderma yunnanensis )CCTCC NO: M 2025645 is a high-yield siderophore strain with a siderophore production activity of 69.79%, capable of synthesizing and secreting carboxylic acid siderophores; and the strain has sulfur oxidation function; In addition, the present invention also provides the use of the Trichoderma in regulating plant diseases, and the Trichoderma of the present invention can antagonize Fusarium solani ( Fusarium solani ), Fusarium graminearum ( Fusarium gramineae )、Fusarium rotundifolia( Fusarium concentricum ) and brown crustacean ( Rosellinia necatrix ), Alternaria brassicae ( Alternaria brassicae (Fr.) Keissler); The Yunnan Trichoderma fungus provided by the present invention is a strain isolated from tobacco-growing soil. Compared with strains screened in extreme environments and laboratory mutagenesis, it has better adaptability to the planting environment and can stably colonize in the rhizosphere and soil of plants, thereby exerting a long-term and stable growth-promoting effect. It is safe and environmentally friendly and has broad application prospects.

The application belongs to the field of agricultural microorganism technology, and discloses bacillus velezensis Bac8 and application thereof in prevention and treatment of black spot disease of cabbage. The strain is isolated from soil of a cabbage planting field, and has been preserved in China Center for Type Culture Collection on February 28, 2026, with a preservation number of CCTCC NO: M 2026354. The strain has significant antagonistic effect on alternaria brassicae, and can be used for development of a biocontrol product, and has a good application prospect in prevention and treatment of black spot disease of cabbage caused by alternaria brassicae.

The application discloses an indoor high-efficiency identification method of Chinese cabbage black spot induced by Leptosphaeria maculans, and comprises the following steps: S1, Chinese cabbage seedling culture; S2, preparation of spore suspension; S3, inoculation of test materials; and S4, culture and investigation of the test materials. Compared with the prior art, the application has the advantages of significantly shortened identification period, realized high-efficiency screening, high controllability of environment, high repeatability of results, reduced risk of misjudgment, saved resource cost and consistent green and sustainable agricultural development direction.