Tea saponin bactericide and application thereof

Abstract

The invention discloses a tea saponin bactericide. The active components in the bactericide comprise tea saponin, a microbial complex microbial inoculant and an auxiliary agent. By adding the paenibacillus, the bacillus amyloliquefaciens, the gluconacetobacter xylinum and the tea saponin into the bactericide, the activity of the navel orange colletotrichum gloeosporioides and the navel orange alternaria brassicae can be effectively inhibited.

Description

Technical Field

[0001] The invention relates to the technical field of fungicides, and in particular to a tea saponin fungicide and application thereof. Background Art

[0002] Navel orange (scientific name: Citrus sinensis Osb.var.brasliliensis Tanaka) is a cultivated variety of sweet orange of the Rutaceae family (Rutaceae) and Citrus genus (Citrus L.). It is named navel orange because of the underdeveloped secondary fruitlets attached to the top of the fruit and the navel-shaped crack on the top of the fruit.

[0003] During the cultivation of navel oranges, navel orange anthracnose is a common disease that can occur in every part of the plant. In severe cases, it often causes a large number of leaves to fall off, the branches and shoots of the plant to die, and there will be stunted and fallen fruits, causing the tree to decline, ultimately affecting the navel orange yield and even causing the death of the entire plant. During the storage and transportation process after harvest, a large number of fruits may also rot.

[0004] Navel orange black spot is a fungal disease that attacks navel oranges and mainly harms fruits and leaves. It is divided into two types: black spot type and black star type. It mainly occurs within one and a half months after the flowering period or flower fall, and is prone to cause fruit rot. The harmful fungi are citrus sphaerocephala and citrus stem spot, which belong to the subphylum Ascomycota (the sexual state is the subphylum Deuteromycota).

[0005] Anthracnose and black spot of navel orange can be controlled by field preparation and pesticide spraying. For a long time, the application of chemical pesticides has been the most effective means of controlling anthracnose and black spot of navel orange. However, as people's awareness of green food gradually increases, the abuse of pesticides does not meet people's general pursuit of green food. Therefore, microbial fungicides are the natural choice to inhibit plant diseases and insect pests.

[0006] Therefore, it is urgent to develop a fungicide for preventing and treating navel orange anthracnose and navel orange black spot. Summary of the invention

[0007] In view of this, the present invention discloses a tea saponin fungicide and application thereof, which can effectively solve the above-mentioned problems existing in the prior art.

[0008] To achieve the above object, the technical solution of the present invention is to provide

[0009] A tea saponin fungicide, wherein the active components of the fungicide include tea saponin, a microbial composite agent and an auxiliary agent.

[0010] Furthermore, the microbial composite agent is at least one or more of Paenibacillus, Bacillus amyloliquefaciens and Gluconobacter xylinus.

[0011] Furthermore, the microbial composite agent includes a mixture of Paenibacillus, Bacillus amyloliquefaciens and Gluconic Acetobacter xylinum, and the weight ratio of Paenibacillus, Bacillus amyloliquefaciens and Gluconic Acetobacter xylinum in the microbial composite agent is 1:1:2.

[0012] Furthermore, the auxiliary agent is at least one of an emulsifier, a wetting and dispersing agent, an antifreeze agent, a preservative, a thickener, a defoaming agent and a solvent.

[0013] Preferably, the auxiliary agent includes an emulsifier, a wetting and dispersing agent, an antifreeze agent, a preservative, a thickener, a defoaming agent and a solvent.

[0014] Furthermore, the solvent is at least one or more selected from propanol, isopropanol, propylene glycol, glycerol, n-butanol, isobutanol, tert-butanol, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol dimethyl ether, ethyl acetate, mineral oil, aromatic hydrocarbon solvent oil and methyl oleate.

[0015] Furthermore, the emulsifier is at least one or more of calcium dodecylbenzene sulfonate, styrylphenol polyoxyethylene ether phosphate, benzylphenol polyoxyethylene ether phosphate, polyethylene glycol monostearate, glycerol monostearate, dibenzylphenol polyoxyethylene ether, polyoxyethylene lauryl ether, polyoxyethylene sorbitan monolaurate, polyoxyethylene monopalmitate, styrylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil polyoxyethylene ether and cardanol polyoxyethylene ether.

[0016] Furthermore, the wetting and dispersing agent is selected from at least one of lignin sulfonate, sodium polycarboxylate, naphthalene sulfonate, sodium dodecyl sulfate, sodium octadecyl sulfosuccinate, alkylphenol ethoxylate, EO-PO block copolymer, fatty alcohol polyoxyethylene ether phosphate, and fatty alcohol ethoxylate.

[0017] Furthermore, the antifreeze agent is at least one or more selected from ethylene glycol, 1,2-propylene glycol, glycerol, polyethylene glycol, sorbitol, sodium chloride, ethylene glycol butyl ether, ethylene glycol butyl ether acetate, diethylene glycol, triethylene glycol, and polyethylene glycol;

[0018] The preservative is at least one selected from the group consisting of formaldehyde, isothiazolinone, benzoic acid, sodium benzoate, sorbic acid and potassium sorbate;

[0019] The thickener is one or more of glycerol, xanthan gum, sodium alginate, magnesium aluminum silicate, polyvinyl alcohol, hydroxyethyl cellulose, modified starch, gelatin, guar gum, sodium carboxymethyl cellulose, organic bentonite, and silicon dioxide;

[0020] The defoaming agent is one or more of polydimethylsiloxane, polyether-modified silicon and higher alcohol.

[0021] Furthermore, by weight, the fungicide includes 10-25 parts of tea saponin, 10-20 parts of microbial composite agent, 3-10 parts of emulsifier, 0.1-5 parts of wetting and dispersing agent, 0.1-5 parts of antifreeze agent, 0.1-3 parts of preservative, 0.1-1 parts of thickener, 0.01-0.2 parts of defoaming agent and 10-30 parts of solvent.

[0022] Furthermore, by weight, the fungicide includes 15-20 parts of tea saponin, 12-18 parts of microbial composite agent, 5-8 parts of emulsifier, 1.5-4 parts of wetting and dispersing agent, 1.5-3.5 parts of antifreeze agent, 1-2 parts of preservative, 0.2-0.8 parts of thickener, 0.1-0.18 parts of defoaming agent and 15-25 parts of solvent.

[0023] Furthermore, by weight, the bactericide includes 18 parts of tea saponin, 16 parts of microbial composite agent, 7 parts of emulsifier, 3 parts of wetting and dispersing agent, 2.5 parts of antifreeze agent, 1.5 parts of preservative, 0.5 parts of thickener, 0.1 parts of defoaming agent and 20 parts of solvent.

[0024] Furthermore, the emulsifier is calcium dodecylbenzene sulfonate;

[0025] The wetting and dispersing agent is sodium octadecyl sulfosuccinate;

[0026] The antifreeze agent is polyethylene glycol; the preservative is sodium benzoate; the thickener is magnesium aluminum silicate; the defoaming agent is polydimethylsiloxane; and the solvent is isobutyl alcohol.

[0027] Furthermore, the formulation of the fungicide composition is one of a suspension, a powder, a water-dispersible granule, a dispersible oil suspension and a suspoemulsion.

[0028] Furthermore, tea saponin fungicide can be used to inhibit navel orange black spot bacteria and navel orange anthracnose bacteria.

[0029] The advantages and beneficial effects of the present invention are that the present invention can effectively inhibit the activity of navel orange anthracnose bacteria and navel orange black spot bacteria by adding Paenibacillus, Bacillus amyloliquefaciens, Gluconobacter xylonacetobacter and tea saponin into the fungicide. DETAILED DESCRIPTION

[0030] The specific implementation of the present invention is further described below in conjunction with the examples. The following examples are only used to more clearly illustrate the technical solutions of the present invention, and cannot be used to limit the protection scope of the present invention.

[0031] The preservation information of each strain in the microbial composite agent of the present invention is as follows:

[0032] Paenibacillus sp was deposited in the General Microbiology Center of China Culture Collection Administration on April 9, 2021, with the deposit number CGMCC No.22158.

[0033] Bacillus amyloliquefaciens was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on January 18, 2013, with the deposit number of CGMCC No.7159.

[0034] Gluconacetobacter xylinum 323 was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on July 12, 2004, with the deposit number of CGMCC NO.1186.

[0035] The effective viable counts of Paenibacillus, Bacillus amyloliquefaciens and Xylotreneacetobacter 323 were all 2 billion / g.

[0036] Test 1: Preparation of fungicide

[0037] Example:

[0038] A tea saponin bactericide is prepared from the following raw materials in parts by weight: tea saponin, bacillus, bacillus amyloliquefaciens, gluconic acid acetobacter, emulsifier, wetting dispersant, antifreeze, preservative, thickener, defoamer and solvent.

[0039] Among them, calcium dodecylbenzene sulfonate is selected as the emulsifier;

[0040] The wetting and dispersing agent is sodium octadecyl sulfosuccinate;

[0041] The antifreeze agent is polyethylene glycol; the preservative is sodium benzoate; the thickener is magnesium aluminum silicate; the defoaming agent is polydimethylsiloxane; and the solvent is isobutyl alcohol.

[0042] The weight parts of the ingredients in Examples 1 to 5 are as follows:

[0043] Table 1 - Example 1 - Example 5 Weight parts of each ingredient

[0044] Example 1 Example 2 Example 3 Example 4 Example 5 Tea saponin 18 10 25 15 20 Paenibacillus 4 2.5 5 3 4.5 Bacillus amyloliquefaciens 4 2.5 5 3 4.5 Gluconacetobacter xylinum 8 5 10 6 9 Emulsifier 7 10 3 8 5 Wetting and dispersing agents 3 0.1 5 1.5 4 antifreeze 2.5 5 0.1 3.5 1.5 preservative 1.5 0.1 3 1 2 Thickener 0.5 1 0.1 0.8 0.2 Defoaming agent 0.1 0.01 0.2 0.1 0.18 Solvents 20 30 10 25 15

[0045] Control group:

[0046] A tea saponin bactericide is prepared from the following raw materials in parts by weight: tea saponin, bacillus, bacillus amyloliquefaciens, gluconic acid acetobacter, emulsifier, wetting dispersant, antifreeze, preservative, thickener, defoamer and solvent.

[0047] Among them, calcium dodecylbenzene sulfonate is selected as the emulsifier;

[0048] The wetting and dispersing agent is sodium octadecyl sulfosuccinate;

[0049] The antifreeze agent is polyethylene glycol; the preservative is sodium benzoate; the thickener is magnesium aluminum silicate; the defoaming agent is polydimethylsiloxane; and the solvent is isobutyl alcohol.

[0050] The weight portions of the ingredients in control group 1 to control group 8 are as follows:

[0051] Table 2 - Weight of each ingredient in control group 1 to control group 8

[0052]

[0053]

[0054] Test 2: Antibacterial activity test

[0055] (1) The present invention selects two strains of navel orange anthracnose and navel orange black spot, and uses the mycelium growth rate method (Ann. Appl. Biol., 2008, 152, 369) to determine the antibacterial activity of the compound.

[0056] (2) First, weigh a certain amount of PDA (potato dextrose agar) into a wide-mouth flask, add distilled water to prepare a culture medium and sterilize it in a 120℃ autoclave for half an hour. Weigh 1 mg of the drug to be tested and dissolve it in 10 mL of acetone to prepare a 100 mg / L agent. Then take half of the agent and dilute it to 10 mL with acetone to prepare a 50 mg / L agent. Similarly, you can prepare a 25 mg / L agent. Take 5 mL of the agent and pour it into 50 mL of potato dextrose agar and mix well. Then pour it into 3 high-temperature sterilized culture media respectively. After cooling, use an inoculation loop to inoculate the above pathogens respectively, seal it with a sealing film, and transfer it to an appropriate temperature for observation. When the colony in the blank group without adding the agent reaches 80%, the colony of the experimental group is measured by the cross method. The formula is as follows:

[0057] Inhibition rate = (blank group colony diameter - experimental group colony diameter) / blank group colony diameter × 100%.

[0058] (3) The test results are shown in Table 3 below:

[0059] Table 3 - Inhibition rate of pathogens in the examples and control groups (unit / %)

[0060]

[0061] From the above table, it can be seen that: when the fungicides of Examples 1-5 of the present invention are used, Example 1 is the best, and at different concentrations, the inhibition rate of navel orange anthracnose and navel orange black spot can reach 100%; the control groups 1-8 are all poor in antibacterial properties compared with Example 1, especially the control group 1 without adding Paenibacillus, Bacillus amyloliquefaciens, and Xylotrene Acetobacter, indicating that Paenibacillus, Bacillus amyloliquefaciens, and Xylotrene Acetobacter are helpful in inhibiting the activity of navel orange anthracnose and navel orange black spot. It can be seen that the fungicide provided by the present invention can effectively inhibit the activity of navel orange anthracnose and navel orange black spot.

[0062] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A tea saponin fungicide, characterized in that: The active components in the bactericide include tea saponin, microbial composite agent and auxiliary agent.

2. A tea saponin fungicide according to claim 1, characterized in that: The microbial composite agent is at least one or more of Paenibacillus, Bacillus amyloliquefaciens and Glucose Acetobacter xylinum.

3. A tea saponin fungicide according to claim 2, characterized in that: The microbial composite bacterial agent comprises a mixture of Paenibacillus, Bacillus amyloliquefaciens and Gluconic Acetobacter xylinum, and the weight ratio of Paenibacillus, Bacillus amyloliquefaciens and Gluconic Acetobacter xylinum in the microbial composite bacterial agent is 1:1:

2.

4. A tea saponin fungicide according to claim 1, characterized in that: The auxiliary agent is at least one or more of an emulsifier, a wetting and dispersing agent, an antifreeze agent, a preservative, a thickener, a defoaming agent and a solvent.

5. A tea saponin fungicide according to claim 4, characterized in that: The emulsifier is at least one of calcium dodecylbenzene sulfonate, polyoxyethylene styrylphenol ether phosphate, polyoxyethylene benzylphenol ether phosphate, polyethylene glycol monostearate, glycerol monostearate, polyoxyethylene dibenzylphenol ether, polyoxyethylene lauryl alcohol ether, polyoxyethylene sorbitan monolaurate, polyoxyethylene monopalmitate, polyoxyethylene styrylphenol ether, fatty alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether, castor oil polyoxyethylene ether and cardanol polyoxyethylene ether.

6. A tea saponin fungicide according to claim 4, characterized in that: The wetting and dispersing agent is selected from at least one of lignin sulfonate, sodium polycarboxylate, naphthalene sulfonate, sodium dodecyl sulfate, sodium octadecyl sulfosuccinate, alkylphenol ethoxylate, EO-PO block copolymer, fatty alcohol polyoxyethylene ether phosphate, and fatty alcohol ethoxylate.

7. A tea saponin fungicide according to claim 4, characterized in that: The antifreeze agent is at least one or more selected from ethylene glycol, 1,2-propylene glycol, glycerol, polyethylene glycol, sorbitol, sodium chloride, ethylene glycol butyl ether, ethylene glycol butyl ether acetate, diethylene glycol, triethylene glycol, and polyethylene glycol; the preservative is at least one or more selected from formaldehyde, isothiazolinone, benzoic acid, sodium benzoate, sorbic acid, and potassium sorbate.

8. A tea saponin fungicide according to claim 4, characterized in that: The thickener is one or more of glycerol, xanthan gum, sodium alginate, magnesium aluminum silicate, polyvinyl alcohol, hydroxyethyl cellulose, modified starch, gelatin, guar gum, sodium carboxymethyl cellulose, organic bentonite, and silicon dioxide; the defoamer is one or more of polydimethylsiloxane, polyether modified silicon, and high carbon alcohol.

9. A tea saponin fungicide according to claim 1, characterized in that: The dosage form of the fungicide composition is one of suspension, powder, water-dispersible granule, dispersible oil suspension and suspoemulsion.

10. Use of the tea saponin fungicide as claimed in any one of claims 1 to 9 in inhibiting navel orange black spot pathogen and navel orange anthracnose pathogen.

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