A composition for regulating skin microecology containing biological fermentation products and its application

Abstract

The present invention relates to the field of cosmetic technology, and in particular to a composition for regulating skin microecology containing a biological fermentation product and its application. The composition comprises inulin, micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soybean milk fermentation product filtrate and water. The composition can effectively adjust the balance of skin microecology, and has excellent effects of removing wrinkles, preventing aging, and moisturizing.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition for regulating skin microecology containing a biological fermentation product and application thereof. Background Art

[0002] Skin microecology refers to an ecosystem composed of various microorganisms such as bacteria, fungi, viruses, mites and arthropods, as well as tissues, cells, various secretions and microenvironments on the surface of the skin. The interaction between skin microorganisms, the host and the external environment constitutes the balance of the skin microecology. Skin microorganisms are important members of the skin microecology system. The flora on the surface of the skin can usually be divided into resident bacteria and transient bacteria. Transient bacteria refer to a type of microorganisms acquired through contact with the external environment. This type is the main pathogen that causes skin infections, including Staphylococcus aureus, Pseudomonas aeruginosa, etc.

[0003] Skin aging and becoming rough and dry are also caused by the disruption of the skin microecology; the microecology of aged skin has changed greatly compared to that of young skin, and also affects the physiological state of the skin, including parameters such as skin elasticity, firmness, and skin sensitivity. Existing cosmetics or cosmetic additives rarely solve the technical problems of skin microecology and skin wrinkles, looseness, dehydration, and dryness at the same time.

[0004] Therefore, providing a composition to achieve the purpose of regulating skin microecology and having both high-efficiency wrinkle removal, anti-aging and moisturizing is one of the key technical issues currently being addressed. Summary of the invention

[0005] The present invention provides a composition for regulating skin microecology containing a biological fermentation product and a related technical solution for its application, so as to solve the technical problems of skin microecology imbalance and skin wrinkles, aging, dryness and dehydration.

[0006] In one aspect, the present invention provides a composition for regulating skin microecology containing a biological fermentation product, wherein the composition comprises inulin, micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate and water.

[0007] Preferably, the amount of inulin used, by mass, is 0.1-1 part, 0.2-1 part or 0.3-0.8 part.

[0008] Preferably, the amount of the micrococcal lysate used, by mass, is 0.5-3.5 parts, 0.8-3.4 parts or 1-3 parts.

[0009] Preferably, the amount of the bifid yeast fermentation product lysate used, by mass, is 0.5-3.5 parts, 0.6-3 parts or 0.8-3 parts.

[0010] Preferably, the amount of the rice fermentation product filtrate used, by mass, is 2-6 parts, 2.5-5.8 parts or 2.5-5.5 parts.

[0011] Preferably, the amount of the lactobacillus / soymilk fermentation product filtrate used is 0.3-1.5 parts, 0.4-1.3 parts or 0.5-1.2 parts by mass.

[0012] Preferably, the amount of water used, by mass, is 10-100 parts, 12-90 parts or 15-65 parts.

[0013] Preferably, the composition for regulating skin microecology containing biological fermentation products comprises, by mass, 0.1-1 parts of inulin, 0.5-3.5 parts of micrococcal lysate, 0.5-3.5 parts of bifid yeast fermentation product lysate, 2-6 parts of rice fermentation product filtrate, 0.3-1.5 parts of lactobacillus / soy milk fermentation product filtrate and 5-30 parts of water.

[0014] Preferably, the composition for regulating skin microecology containing biological fermentation products comprises, by mass, 0.2-1 parts of inulin, 0.8-3.4 parts of micrococcal lysate, 0.6-3 parts of bifid yeast fermentation product lysate, 2.2-5.8 parts of rice fermentation product filtrate, 0.4-1.3 parts of lactobacillus / soy milk fermentation product filtrate and 8-25 parts of water.

[0015] Preferably, the mass ratio of the micrococcal lysate to the bifid yeast fermentation product lysate is (1-1.5):1, (1.05-1.5):1 or (1.1-1.4):1.

[0016] Preferably, the composition further contains konjac glucomannan in an amount of 0.2-1.2 parts, 0.3-1 parts or 0.4-1 parts by mass.

[0017] Preferably, the mass ratio of the rice fermentation product filtrate to konjac glucomannan is (5-8):1, (5.5-7.5):1 or (5.5-7):1.

[0018] Preferably, the composition for regulating skin microecology containing biological fermentation products comprises, by mass, 0.1-1 parts of inulin, 0.5-3.5 parts of micrococcal lysate, 0.5-3.5 parts of bifid yeast fermentation product lysate, 2-6 parts of rice fermentation product filtrate, 0.3-1.5 parts of lactobacillus / soy milk fermentation product filtrate, 0.2-1.2 parts of konjac glucomannan and 10-100 parts of water.

[0019] Preferably, the composition for regulating skin microecology containing biological fermentation products includes, by mass, 0.2-1 parts of inulin, 0.8-3.4 parts of micrococcal lysate, 0.6-3 parts of bifid yeast fermentation product lysate, 2.2-5.8 parts of rice fermentation product filtrate, 0.4-1.3 parts of lactobacillus / soy milk fermentation product filtrate, 0.3-1 parts of konjac glucomannan and 12-90 parts of water.

[0020] On the other hand, the present invention also provides a method for preparing the composition for regulating skin microecology containing biological fermentation products, comprising the following steps: adding water to a container, adding inulin and konjac glucomannan and stirring evenly, then adding micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, continuing stirring, and sterilizing to obtain the composition.

[0021] Preferably, the stirring speed is 50-500 rpm and the stirring time is 0.1-5 hours.

[0022] Preferably, the sterilization is ultraviolet sterilization.

[0023] Preferably, a standing treatment is performed before the sterilization.

[0024] Preferably, the standing temperature is room temperature.

[0025] On the other hand, the present invention also provides use of the composition in preparing cosmetics.

[0026] Preferably, the cosmetics include lotions, essences, creams, pastes, foundations, facial masks, facial cleansers, and shampoo products.

[0027] On the other hand, the present invention also provides use of the composition in preparing cosmetics for regulating skin microecology.

[0028] On the other hand, the present invention also provides a cosmetic comprising the above composition, acrylates / C10-30 alkyl acrylate cross-linked polymer, glycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizate, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone, arginine and water.

[0029] Preferably, the cosmetic comprises, calculated by mass percentage, 2-25% of the composition of any one of claims 1-4, 0.2-0.8% of acrylates / C10-30 alkyl acrylate cross-linked polymer, 2-5% of glycerol, 0.2-1.5% of sodium hyaluronate, 0.2-1.5% of allantoin, 0.2-1% of dipotassium glycyrrhizate, 0.5-3% of butylene glycol, 0.08-0.6% of 1,2-hexanediol, 0.08-0.6% of p-hydroxyacetophenone, 0.2-1% of arginine and the remainder of water.

[0030] On the other hand, the present invention also provides a method for preparing the cosmetic, comprising: adding water into a container, adding the composition, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, glycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizate, butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone and arginine, stirring and mixing, and sterilizing to obtain the cosmetic.

[0031] Preferably, the stirring speed is 50-500 rpm and the stirring time is 0.1-5 hours.

[0032] Preferably, the sterilization is ultraviolet sterilization.

[0033] Preferably, a standing treatment is performed before the sterilization.

[0034] Preferably, the standing temperature is room temperature.

[0035] Beneficial effects:

[0036] The composition of the present invention can effectively solve the technical problems of skin microecological imbalance and the occurrence of wrinkles, aging, dryness and water deficiency on the skin, and efficiently imparts skin microecological balance and the effects of removing wrinkles, preventing aging and moisturizing the skin.

[0037] When the composition of the present invention is diluted to 1.5% by mass, the diameter of the inhibition zone for inhibiting Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Aspergillus niger is in the range of 10-15 mm. The composition of the present invention can effectively inhibit the growth and survival of harmful bacteria on the skin and give the skin a healthy state.

[0038] The composition of the present invention reduces the proportion of crow's feet by 26.43% after one month of use, and reduces the proportion of crow's feet by 38.27% after three months of use. The composition of the present invention has excellent anti-aging and wrinkle-removing effects and gives the skin a firm state.

[0039] The micrococcal lysate and the bifid yeast fermentation product lysate of the present invention can give the composition a highly effective anti-aging and wrinkle-removing effect. The combination of the two produces a mutually reinforcing relationship and can synergistically increase the effect of the composition in removing crow's feet. When the ratio of the two is in the range of (1-1.5):1, the wrinkle-removing effect of the obtained composition is more significant.

[0040] The composition of the present invention can increase the skin's moisture content by 48.7% after 3 hours of use, and can still increase the skin's moisture content by 27.5% after 8 hours of use. It has highly efficient instantaneous moisturizing and long-lasting moisturizing effects, giving the skin a continuous plump and hydrated state.

[0041] The rice fermentation product filtrate and konjac glucomannan in the composition of the present invention can both impart a good moisturizing and hydrating effect to the combination; and when the ratio of the two is in the range of 5-8:1, the composition shows a better moisturizing effect. DETAILED DESCRIPTION

[0042] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0043] Micrococcal lysate: Xiamen Xulijin Biotechnology Co., Ltd. Bifida yeast fermentation product lysate: Xi'an Prius Bioengineering Co., Ltd. Rice fermentation product filtrate: Hubei Maidehao Chemical Co., Ltd. Lactobacillus / soy milk fermentation product filtrate: Jining Fangyu Chemical Co., Ltd. Konjac glucomannan: Shaanxi Xiazhou Biotechnology Co., Ltd.

[0044] 1. Preparation of test composition

[0045] Composition 1

[0046] The raw materials are composed of: 0.8 parts of inulin, 2.6 parts of micrococcal lysate, 2 parts of bifid yeast fermentation product lysate, 4.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.8 parts of konjac glucomannan and 45 parts of water; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 1.3:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 6:1.

[0047] The specific preparation steps are as follows: adding water to a container, adding inulin and konjac glucomannan, stirring at 80 rpm for 0.5 hour, stirring evenly, then adding micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, maintaining the speed and stirring for 0.2 hour, and ultraviolet sterilization to obtain the composition 1.

[0048] Composition 2

[0049] The raw materials are composed of: 0.75 parts of inulin, 2.3 parts of micrococcal lysate, 2.3 parts of bifid yeast fermentation product lysate, 4.97 parts of rice fermentation product filtrate, 1.05 parts of lactobacillus / soy milk fermentation product filtrate, 0.63 parts of konjac glucomannan and 45.5 parts of water; the amount ratio of micrococcal lysate and bifid yeast fermentation product lysate is 1:1, and the amount ratio of rice fermentation product filtrate and konjac glucomannan is 7.89:1.

[0050] The specific preparation steps are as follows: adding water to a container, adding inulin and konjac glucomannan, stirring at 100 rpm for 0.4 hour, stirring evenly, then adding micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, stirring at 80 rpm for 0.3 hour, and ultraviolet sterilization to obtain the composition 2.

[0051] Composition 3

[0052] The raw materials are composed of: 0.8 parts of inulin, 4.6 parts of bifid yeast fermentation product lysate, 4.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.8 parts of konjac glucomannan and 45 parts of water; the ratio of rice fermentation product filtrate to konjac glucomannan is 6:1.

[0053] The specific preparation steps are as follows: adding water to a container, adding inulin and konjac glucomannan, stirring at 80 rpm for 0.5 hour, stirring evenly, then adding the bifid yeast fermentation product lysate, rice fermentation product filtrate, and lactobacillus / soy milk fermentation product filtrate, maintaining the speed and stirring for 0.2 hour, and ultraviolet sterilization to obtain the composition 3.

[0054] Composition 4

[0055] The raw materials are composed of: 0.8 parts of inulin, 4.6 parts of micrococcal lysate, 4.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.8 parts of konjac glucomannan and 45 parts of water; the ratio of rice fermentation product filtrate to konjac glucomannan is 6:1.

[0056] The specific preparation steps are as follows: water is added into a container, inulin and konjac glucomannan are added, and the mixture is stirred at 80 rpm for 0.5 hour until uniformly mixed. Then, micrococcal lysate, rice fermentation product filtrate, and lactobacillus / soymilk fermentation product filtrate are added, stirring is continued for 0.2 hour while maintaining the rotation speed, and ultraviolet sterilization is performed to obtain the composition 4.

[0057] Composition 5

[0058] The raw materials are composed of: 0.8 parts of inulin, 1.6 parts of micrococcal lysate, 3 parts of bifid yeast fermentation product lysate, 4.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.8 parts of konjac glucomannan and 45 parts of water; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 0.53:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 6:1.

[0059] The specific preparation steps are as follows: water is added into a container, inulin and konjac glucomannan are added, and the mixture is stirred at 80 rpm for 0.5 hour until uniformly mixed. Then, micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, and lactobacillus / soymilk fermentation product filtrate are added, stirring is continued for 0.2 hour while maintaining the rotation speed, and ultraviolet sterilization is performed to obtain the composition 5.

[0060] Composition 6

[0061] The raw materials are composed of: 0.8 parts of inulin, 3.1 parts of micrococcal lysate, 1.5 parts of bifid yeast fermentation product lysate, 4.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.8 parts of konjac glucomannan and 45 parts of water by weight; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 2.07:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 6:1.

[0062] Specific preparation steps: add water into a container, add inulin and konjac glucomannan, stir at 80 rpm for 0.5 hour, stir evenly, then add micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, maintain the speed and continue stirring for 0.2 hour, and sterilize with ultraviolet light to obtain the composition 6.

[0063] Composition 7

[0064] The raw materials are composed of: 0.8 parts of inulin, 2.6 parts of micrococcal lysate, 2 parts of bifid yeast fermentation product lysate, 2.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 2.8 parts of konjac glucomannan and 45 parts of water by weight; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 1.3:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 1:1.

[0065] The specific preparation steps are as follows: water is added into a container, inulin and konjac glucomannan are added, and the mixture is stirred at 80 rpm for 0.5 hour until uniformly mixed. Then, micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, and lactobacillus / soy milk fermentation product filtrate are added, stirring is continued for 0.2 hour while maintaining the rotation speed, and ultraviolet sterilization is performed to obtain the composition 7.

[0066] Composition 8

[0067] The raw materials are composed of: 0.8 parts of inulin, 2.6 parts of micrococcal lysate, 2 parts of bifid yeast fermentation product lysate, 3.8 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 1.8 parts of konjac glucomannan and 45 parts of water by weight; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 1.3:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 2.1:1.

[0068] The specific preparation steps are as follows: add water into a container, add inulin and konjac glucomannan, stir at 80 rpm for 0.5 hour, stir evenly, then add micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, maintain the speed and continue stirring for 0.2 hour, and sterilize with ultraviolet light to obtain the composition 8.

[0069] Composition 9

[0070] The raw materials are composed of: 0.8 parts of inulin, 2.6 parts of micrococcal lysate, 2 parts of bifid yeast fermentation product lysate, 5.2 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.4 parts of konjac glucomannan and 45 parts of water; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 1.3:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 13:1.

[0071] The specific preparation steps are as follows: water is added into a container, inulin and konjac glucomannan are added, and the mixture is stirred at 80 rpm for 0.5 hour to be evenly stirred. Then, micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, and lactobacillus / soy milk fermentation product filtrate are added, stirring is continued for 0.2 hour while maintaining the rotation speed, and ultraviolet sterilization is performed to obtain the composition 9.

[0072] Composition 10

[0073] The raw materials are composed of: 0.8 parts of inulin, 2.6 parts of micrococcal lysate, 2 parts of bifid yeast fermentation product lysate, 5.4 parts of rice fermentation product filtrate, 1 part of lactobacillus / soy milk fermentation product filtrate, 0.2 parts of konjac glucomannan and 45 parts of water; the ratio of micrococcal lysate to bifid yeast fermentation product lysate is 1.3:1, and the ratio of rice fermentation product filtrate to konjac glucomannan is 27:1.

[0074] The specific preparation steps are as follows: adding water to a container, adding inulin and konjac glucomannan, stirring at 80 rpm for 0.5 hour, stirring evenly, then adding micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate, maintaining the speed and stirring for 0.2 hour, and ultraviolet sterilization to obtain the composition 10.

[0075] 2. Testing

[0076] 1. Regulate skin microecology test

[0077] The above composition 1-2 was prepared with sterile water to prepare a composition group 1-2 with a mass concentration of 1.5% as a test sample.

[0078] Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Aspergillus niger are common adverse bacteria on the skin surface. The inhibitory effect of the above bacteria is used to characterize the effect of the composition in regulating the skin microecological barrier.

[0079] Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Aspergillus niger were selected and made into bacterial suspension using PBS. The bacterial concentration was 6.2×10 6 CFU / mL.

[0080] Oxford cup method to test the antibacterial zone: absorb 100μL of the above bacterial suspension on the surface of the coagulation medium, spread it evenly and cover the plate, and let it stand at 20℃ for 10min; invert the sterile Oxford cup on the surface of the culture medium, add the sample of the combination group until the liquid surface is parallel to the surface of the Oxford cup, and culture it in the incubator at 37℃ for 18h. Then test the antibacterial zone to determine the antibacterial effect. Among them, the antibacterial zone diameter > 20mm is marked with ++++; the antibacterial zone diameter is 15-20mm, marked with +++; the antibacterial zone diameter is 10-15mm, marked with ++; the antibacterial zone diameter <10mm is marked with +; the antibacterial zone diameter is 0mm, marked with -. The specific test results are shown in Table 1.

[0081] Table 1 Skin microecology regulation test

[0082] Test / Bacteriostasis Pseudomonas aeruginosa Staphylococcus aureus Escherichia coli Aspergillus niger Composition 1 ++ ++ ++ ++ Composition 2 ++ ++ ++ ++

[0083] From the test results in Table 1, it can be seen that the composition of the present invention has a good inhibitory effect on Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Aspergillus niger, can well control harmful bacteria on the skin, give the skin a healthy state, and has an excellent effect of regulating the skin microecology.

[0084] 2. Anti-aging and anti-wrinkle test

[0085] The 36 volunteers recruited were randomly divided into 6 groups, each group had 3 males and 3 females, all aged 40-55 years old. The test samples were the above-mentioned compositions 1-6. The test method was: each group of volunteers applied the above-mentioned composition once every night on the face (including the crow's feet area), applied evenly, about 2g each time, and used it continuously for 2 months. The 3D skin imaging system PR I MOS-CR was used to take 3D images of the local skin of the eye corners of the volunteers before using the test sample, after using the sample for 1 month, and after using the sample for 2 months; then the software was used to analyze and convert the reduction ratio of the number of crow's feet. The reduction ratio β value of the number of crow's feet after using it for 1 month and 2 months was used as the anti-aging and wrinkle removal effect; wherein, the β value after using it for 1 month = [(the number of crow's feet before using the sample - the number of crow's feet after using the sample for 1 month) / the number of crow's feet before using the sample] × 100%, and the β value after using it for 2 months = [(the number of crow's feet before using the sample - the number of crow's feet after using the sample for 2 months) / the number of crow's feet before using the sample] × 100%. The results are shown in Table 2.

[0086] Table 2 Anti-aging and anti-wrinkle test

[0087]

[0088]

[0089] 3. Moisturizing and hydrating test

[0090] 24 volunteers were selected and randomly divided into 6 groups, with 2 males and 2 females in each group, all aged 40-55 years old. The test samples were the above-mentioned compositions 1-2, 7-10. Test instrument: Skin moisture tester CorneometerCM825. Test area: the same 3×3 inner area on the inside of the right arm. Sample use method: Each group of volunteers applied the test sample evenly on the test area, and the amount used was 0.06g. Test method: Test the skin moisture content of the test area before use and 3 and 8 hours after use, and take three different positions for testing each time, and take the average value; then calculate the skin moisture content increase ratio η value after 3 and 8 hours of use. Among them, the η value after 3 hours of use = [(skin moisture content of the sample after 3 hours of use - skin moisture content before using the sample) / skin moisture content before using the sample] × 100%; the η value after 8 hours of use = [(skin moisture content of the sample after 8 hours of use - skin moisture content before using the sample) / skin moisture content before using the sample] × 100%. The results are shown in Table 3.

[0091] Table 3 Moisturizing and hydrating test

[0092]

[0093]

[0094] It can be seen from the test results in Tables 1-3 above that the composition of the present invention can effectively solve the technical problems of skin microecological imbalance and the occurrence of wrinkles, aging, dryness and dehydration on the skin, and efficiently imparts skin microecological balance and the effects of removing wrinkles, preventing aging, and moisturizing the skin.

[0095] From the test results in Table 1, it can be seen that when the composition of the present invention is diluted to 1.5% by mass, the diameter of the inhibition zone for inhibiting Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, and Aspergillus niger is in the range of 10-15 mm. The composition of the present invention can effectively inhibit the growth and survival of harmful bacteria on the skin and give the skin a healthy state.

[0096] From the test results in Table 2, it can be seen that the composition of the present invention reduces the proportion of crow's feet by 26.43% after one month of use, and reduces the proportion of crow's feet by 38.27% after three months of use. The composition of the present invention has excellent anti-aging and anti-wrinkle effects and gives the skin a firm state.

[0097] From the crow's feet removal effects of compositions 1, 3-4 in Table 2, it can be seen that the micrococcal lysate and the bifid yeast fermentation product lysate of the present invention can give the composition a highly effective anti-aging and wrinkle removal effect, and the combination of the two produces a mutually reinforcing relationship, which can synergistically increase the crow's feet removal effect of the composition. When the ratio of the two is in the range of (1-1.5):1, the wrinkle removal effect of the obtained composition is more significant.

[0098] From the test results in Table 3, it can be seen that the composition of the present invention can increase the skin moisture content by 48.7% after 3 hours of use, and can still increase the skin moisture content by 27.5% after 8 hours of use. It has efficient instant moisturizing and long-lasting moisturizing effects, giving the skin a continuous plump and hydrated state.

[0099] The test results of compositions 1, 7-10 in Table 3 show that both rice fermentation product filtrate and konjac glucomannan can impart a good moisturizing effect to the combination; and when the ratio of the two is in the range of 5-8:1, the composition shows a better moisturizing effect.

[0100] 3. Application

[0101] Cosmetic 1: raw materials in percentage by mass, the above composition 1: 5%; acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer: 0.5%; glycerin: 3%; sodium hyaluronate: 0.5%; allantoin: 1%; dipotassium glycyrrhizate: 0.5%; 1,3-butylene glycol: 2%; 1,2-hexanediol: 0.4%; p-hydroxyacetophenone: 0.3%; arginine: 0.4% and the balance water.

[0102] Preparation steps: add water into a container, add composition 1, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, glycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, 1,3-butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone and arginine, stir and mix at 100 rpm for 0.5 hour, and sterilize with ultraviolet light to obtain the cosmetic 1.

[0103] Cosmetic 2: raw materials, calculated by mass percentage, the above composition 1: 7%; acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer: 0.2%; glycerin: 2.7%; sodium hyaluronate: 1%; allantoin: 1.2%; dipotassium glycyrrhizate: 0.7%; 1,3-butylene glycol: 2.5%; 1,2-hexanediol: 0.4%; p-hydroxyacetophenone: 0.4%; arginine: 0.5% and the balance water.

[0104] Preparation steps: add water into a container, add composition 1, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, glycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizate, 1,3-butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone and arginine, stir and mix at 120 rpm for 0.4 hour, and sterilize with ultraviolet light to obtain the cosmetic 2.

[0105] Cosmetic 3: raw materials, calculated by mass percentage, the above composition 1: 3%; acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer: 0.7%; glycerin: 4.5%; sodium hyaluronate: 1.1%; allantoin: 1.3%; dipotassium glycyrrhizate: 0.75%; 1,3-butanediol: 3%; 1,2-hexanediol: 0.35%; p-hydroxyacetophenone: 0.35%; arginine: 0.4% and the balance water.

[0106] Preparation steps: add water into a container, add composition 1, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, glycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, 1,3-butylene glycol, 1,2-hexanediol, p-hydroxyacetophenone and arginine, stir and mix at 75 rpm for 0.6 hour, and sterilize with ultraviolet light to obtain the cosmetic 3.

[0107] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. Use of a composition for removing crow's feet in preparing cosmetics, characterized in that: The composition comprises, by weight, 0.1-1 parts of inulin, 0.5-3.5 parts of micrococcal lysate, 0.5-3.5 parts of bifid yeast fermentation product lysate, 2-6 parts of rice fermentation product filtrate, 0.3-1.5 parts of lactobacillus / soy milk fermentation product filtrate, 10-100 parts of water and 0.2-1.2 parts of konjac glucomannan; The mass ratio of the rice fermentation product filtrate to konjac glucomannan is (5-8):1; the mass ratio of the micrococcal lysate to the bifid yeast fermentation product lysate is (1-1.5):

1.

2. The use according to claim 1, characterized in that: The composition comprises, by weight, 0.2-1 parts of inulin, 0.8-3.4 parts of micrococcal lysate, 0.6-3 parts of bifid yeast fermentation product lysate, 2.2-5.8 parts of rice fermentation product filtrate, 0.4-1.3 parts of lactobacillus / soy milk fermentation product filtrate, 12-90 parts of water and 0.2-1.2 parts of konjac glucomannan.

3. The use according to claim 1, characterized in that: The preparation method of the composition is as follows: water is added into a container, inulin and konjac glucomannan are added and stirred evenly, then micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate are added, stirring is continued, and sterilization is performed to obtain the composition.

4. Use of a moisturizing and hydrating composition in the preparation of cosmetics, characterized in that: The composition comprises, by weight, 0.1-1 parts of inulin, 0.5-3.5 parts of micrococcal lysate, 0.5-3.5 parts of bifid yeast fermentation product lysate, 2-6 parts of rice fermentation product filtrate, 0.3-1.5 parts of lactobacillus / soy milk fermentation product filtrate, 10-100 parts of water and 0.2-1.2 parts of konjac glucomannan; The mass ratio of the micrococcal lysate to the bifid yeast fermentation product lysate is (1-1.5):1; the mass ratio of the rice fermentation product filtrate to the konjac glucomannan is (5-8):

1.

5. The use according to claim 4, characterized in that: The composition comprises, by weight, 0.2-1 parts of inulin, 0.8-3.4 parts of micrococcal lysate, 0.6-3 parts of bifid yeast fermentation product lysate, 2.2-5.8 parts of rice fermentation product filtrate, 0.4-1.3 parts of lactobacillus / soy milk fermentation product filtrate, 12-90 parts of water and 0.2-1.2 parts of konjac glucomannan.

6. The use according to claim 4, characterized in that: The preparation method of the composition is as follows: water is added into a container, inulin and konjac glucomannan are added and stirred evenly, then micrococcal lysate, bifid yeast fermentation product lysate, rice fermentation product filtrate, lactobacillus / soy milk fermentation product filtrate are added, stirring is continued, and sterilization is performed to obtain the composition.

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