Biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and application of biocontrol strain and agent

A technology for grape downy mildew and pepper blight, which is applied in the application, biocide, fungicide and other directions, can solve problems such as threats to food safety, fruit and environmental pollution, and achieves good control effect, no toxic side effects, and broad application prospects. Effect

Active Publication Date: 2015-03-25
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, the control of grape downy mildew and pepper blight only relies on chemical pesticides, which not only cause serious pollution to fruits and the environmen

Method used

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  • Biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and application of biocontrol strain and agent
  • Biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and application of biocontrol strain and agent
  • Biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and application of biocontrol strain and agent

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Effect test

Embodiment 1

[0026] 1. Strain preparation. Strain Streptomyces niger PY-1 was activated and cultured on PSA medium at a culture temperature of 25-30°C and cultured in the dark for 4 days. PSA medium composition: 200g potato, 20g sucrose, 20g agar, 1000mL distilled water.

[0027] 2. Preparation of biological control agents. Liquid medium ingredients: 200g potato, 20g sucrose, 1000mL distilled water. The filling volume is 200mL (500mL Erlenmeyer flask), and the inoculum volume is 1×10 9 cfu, fermentation temperature 27°C, 150r / min shaking fermentation culture for 5 days, the fermentation liquid was centrifuged at 12000r / min for 30min, and the supernatant was filtered through a 0.45μm filter membrane to prepare a stock solution of biocontrol agents capable of preventing grape downy mildew and pepper blight , add water to dilute 300 times to carry out indoor control test.

[0028] Indoor and in vitro control effect evaluation of grape downy mildew.

[0029] The detached leaves were use...

Embodiment 2

[0039] Evaluation of the control effect of indoor flat panel on the control of Phytophthora capsici by law enforcement.

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Abstract

The invention discloses a biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and an application of the biocontrol strain and agent. The biocontrol strain is characterized by being a streptomyces atratus py-1 strain in surface soil of a grapery and is collected with the number of China General Microbiological Culture Collection Center (CGMCC) No:7826 in CGMCC; and a secondary metabolite of streptomyces atratus py-1 is used as an active component of the biocontrol strain. The biocontrol agent is prepared through the steps of inoculating the streptomyces atratus py-1 into 200ml of psa culture solution in a 500ml triangular flask to culture for 5 days; centrifuging a fermentation solution for 30 minutes; filtering a supernatant solution by using a filter membrane to obtain a stock solution; diluting the stock solution, then, spraying the diluted stock solution on grapes, and irrigating the diluted stock solution into the roots of hot peppers. The biocontrol agent has a certain effect on inhibiting egg plant stem blight, egg plant root rot, tomato spotted wilt virus, tomato late blight, cucumber anthracnose, maize ear rot, sorghum curvularia lunata and wheat root rot.

Description

technical field [0001] The invention relates to a biocontrol strain, a preparation and its application, in particular to a biocontrol strain capable of preventing and treating grape downy mildew and capsicum blight, a preparation and its application, belonging to the technology of biocontrol preparations made from beneficial biocontrol strains field. Background technique [0002] Grape downy mildew is caused by grape downy mildew ( Plasmopara viticola (Berk. and Curt.) Berl. and de Toni) caused by fungal disease, is a worldwide ancient disease, all over the grape producing areas at home and abroad, is one of the main grape leaf diseases. The disease was first discovered on wild grapes in eastern North America. In 1870, Europe introduced American phylloxera-resistant rootstocks, and grape downy mildew entered Europe. A few years later, downy mildew first occurred on a large scale in southern France, and then spread throughout Europe, becoming the primary disease of local gr...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01G7/06C12R1/465
CPCA01G13/00A01N63/10C12N1/205C12R2001/465
Inventor 梁春浩刘长远白元俊关天舒王辉刘丽于舒怡黄玉茜赵达臧超群李柏宏
Owner LIAONING ACAD OF AGRI SCI
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