Biocontrol strain and agent capable of preventing and controlling plasmopara viticola and phytophthora capsici and application of biocontrol strain and agent
A technology for grape downy mildew and pepper blight, which is applied in the application, biocide, fungicide and other directions, can solve problems such as threats to food safety, fruit and environmental pollution, and achieves good control effect, no toxic side effects, and broad application prospects. Effect
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Embodiment 1
[0026] 1. Strain preparation. Strain Streptomyces niger PY-1 was activated and cultured on PSA medium at a culture temperature of 25-30°C and cultured in the dark for 4 days. PSA medium composition: 200g potato, 20g sucrose, 20g agar, 1000mL distilled water.
[0027] 2. Preparation of biological control agents. Liquid medium ingredients: 200g potato, 20g sucrose, 1000mL distilled water. The filling volume is 200mL (500mL Erlenmeyer flask), and the inoculum volume is 1×10 9 cfu, fermentation temperature 27°C, 150r / min shaking fermentation culture for 5 days, the fermentation liquid was centrifuged at 12000r / min for 30min, and the supernatant was filtered through a 0.45μm filter membrane to prepare a stock solution of biocontrol agents capable of preventing grape downy mildew and pepper blight , add water to dilute 300 times to carry out indoor control test.
[0028] Indoor and in vitro control effect evaluation of grape downy mildew.
[0029] The detached leaves were use...
Embodiment 2
[0039] Evaluation of the control effect of indoor flat panel on the control of Phytophthora capsici by law enforcement.
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