Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus amyloliquefaciens strain and applications thereof in inhibiting plasmopara viticola

A technology for grape downy mildew and amyloliquefaciens, which is applied to Bacillus amyloliquefaciens and its application in inhibiting grape downy mildew, and can solve problems such as affecting health, restricting the processing and export of grape products, and destroying the ecological environment. , to achieve the effect of significant application value

Active Publication Date: 2017-03-22
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of grape downy mildew in production is still dominated by chemical control, but the negative impact of "3R" brought about by it is very serious
The extensive use of chemical pesticides has seriously damaged the ecological environment, resulting in a large amount of pesticide residues in grape fruits, affecting people's health
In my country's grape-producing areas, before the grapes are picked, the control methods for grape downy mildew are also mainly chemical pesticides, which not only affects the health of the people, but also limits the processing and export of grape products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus amyloliquefaciens strain and applications thereof in inhibiting plasmopara viticola
  • Bacillus amyloliquefaciens strain and applications thereof in inhibiting plasmopara viticola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, acquisition, identification and preservation of Bacillus amyloliquefaciens KB

[0024] 1. Acquisition of strains

[0025] Collect peaches and kiwifruits from organic orchards in northern China. The strains were isolated from fruits, separated by conventional gradient dilution coating, and cultured on LB solid medium, NA solid medium, KB solid medium and CM0002 solid medium at 28°C respectively. The cultivated colonies were purified and preserved on LB solid medium, and the primary screening and multiple re-screening of antagonistic bacteria were carried out with grape downy mildew as the target pathogen, and finally a strain with strong antibacterial activity was obtained, which was named strain KB.

[0026] 2. Identification of strains

[0027] Refer to the methods described in "Principles and Methods of Soil Microbiology Research" (Lin Xiangui. Principles and Methods of Soil Microbiology Research [M], Higher Education Press, 2010) for the determinatio...

Embodiment 2

[0036] The antibacterial activity of embodiment 2, bacterial strain

[0037] 1. Collect fresh leaves of the grape variety "Red Milk", first rinse with tap water, then sterilize with 75% ethanol aqueous solution for 30 seconds, then rinse with sterile water, and then punch holes with a hole punch to obtain leaf discs with a diameter of 1.5 cm .

[0038] 2. Take the leaf disk obtained in step 1, place it in a petri dish covered with sterile gauze, put the petri dish in a sterile operating table, and blow it for 4-5 minutes.

[0039] 3. Group processing

[0040] Test group: After completing step 2, take the leaf disk and soak it in the biocontrol bacteria solution for 30-60 seconds.

[0041] The preparation method of biocontrol bacteria liquid: Bacillus amyloliquefaciens KB is inoculated to LB liquid culture medium (initial bacterial concentration 10 5 cfu / mL), shake culture at 28-30°C, 180-220rpm for 48 hours, take the whole system, and store it at 4°C for later use.

[0042...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a bacillus amyloliquefaciens strain and applications of the bacillus amyloliquefaciens strain in inhibiting plasmopara viticola. The bacillus amyloliquefaciens KB provided by the invention is assigned with the accession number of CGMCC NO.8179. The cultured product of bacillus amyloliquefaciens KB is also within the protection range of the invention, and the fermented product of bacillus amyloliquefaciens KB is also within the protection range of the invention. The invention further protects the applications of the bacillus amyloliquefaciens KB, the cultured product of bacillus amyloliquefaciens KB, or the fermented product of bacillus amyloliquefaciens KB in inhibiting plasmopara viticola, in preventing and treating plasmopara viticola, and in preventing and treating plant diseases caused by plasmopara viticola. The invention provides the bacillus amyloliquefaciens strain having the inhibiting effect for plasmopara viticola, which has the great application value for preventing and treating plasmopara viticola.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a strain of bacillus amyloliquefaciens and its application in inhibiting grape downy mildew. Background technique [0002] Grape downy mildew is a fungal disease. The widespread occurrence of grape downy mildew has caused a large reduction in fruit production, seriously affecting the edible value and market value of the fruit, resulting in a reduction in the economic income of fruit farmers, and seriously restricting the development of my country's grape industry. No matter in developed countries or in developing countries, due to the lack of necessary effective control agents, the losses caused by grape downy mildew account for more than 20% of the total. The occurrence of grape downy mildew has seriously affected modern grape production, especially the production and processing of high-end grape products. [0003] Grape downy mildew mainly damage...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 刘建斌张殿朋李兴红
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com