33 results about "Bipolaris zeicola" patented technology

The invention discloses a Bacillius subtilis strain, and the culture preservation code for the Bacillius subtilis at China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.1982. Dry-powder microbial inoculum prepared by the strain can be applied to prevention and cure of wheat scab, rice blast, corn southern leaf blight, sclerotium disease germ of garlic, anthracnose germ of cucumber and other germs that cause diseases to agricultural crops and vegetables; the microbial inoculum can also be applied to preservation of fresh fruits such as peaches, apples, grapes, pears, etc. Rice and corn leaves processed with suspension liquid of Bacillus subtilis strain can prevent the corresponding lesion disease; the cucumber seeds or seedlings processed by mixing the seeds with powder, burying the powder around the root or spraying suspension liquid of Bacillus subtilis strain can not only prevent cucumber anthracnose, but also accelerate the growth of the plants. The production of safe and pollution-free vegetables is capable. Fresh peaches processed with suspension liquid of Bacillus subtilis strain are rot-resistance, and the preservation time is prolonged. The preparation process for the microbial inoculum is simple, applicable to commercial production.

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus and elliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention discloses a pseudomonas aeruginosa D10 which is preserved in China Typical Culture Preservation Center, and the preservation number is CCTCC NO: M 209104. A colony on a beef paste peptone culture medium appears brown, round, glossy and wet, and has trim edges; the diameter of the colony is 0.2-0.5 cm; and the thallus is in a short bar shape and does not have gemma. The invention also discloses a preparation method and applications of the pseudomonas aeruginosa D10. The pseudomonas aeruginosa D10 of the invention can inhibit corn northern and southern leaf blight, has strong antergic functions on corn northern leaf blight, corn southern leaf blight, corn curvalaria leaf spot, cotton verticillium wilt, wheat fusarium graminearum, banana colletotrichum toilette and banana fusarium wilt, has the advantages of wide bacteriostatic range, high prevention efficiency and favorable environmental safety, and has favorable prospects for development and applications.

The invention relates to a botanical essential oil for the control of plant pathogenic fungus and a preparation method thereof. The essential oil of the invention is extracted from star aniseseen fruits and contains 29 main ingredients through gas chromatography-mass spectrometry analysis, wherein, trans-anethole accounts for 83.483 percent. The invention first confirms that the trans-anethole is the main antibacterial active ingredient in the essential oil from the star aniseseen as well as the essential oil from the star aniseseen and the trans-anethole thereof has significant inhibition activity for a plurality of plant pathogenic fungus such as early blight of tomato, stem end rot of mango, corn leaf blight, wheat scab, cucumber fusarium wilt, tomato fusarium wilt, cotton fusarium wilt, rice blast fungus, pythium aphani-dermatum, wheat sheath blight, rice sheath blight, etc. and can control plant diseases.

The invention relates to an antagonistic and growth-promoting pseudomonas FRBN3 producing ACC deaminase and an application thereof. The pseudomonas FRBN3 is obtained through separation on surface of watermelon roots from Hefei city, Anhui province and is named Pseudomonas guariconensis. The pseudomonas FRBN3 is preserved in China General Microbiological Culture Collection Center at Apr.14 2015 and is assigned the accession number CGMCC No.10715. The pseudomonas FRBN3 has an ACC deaminase activity and can highly produce siderophores. The pseudomonas FRBN3 has a certain inhibition effect on all of fusarium oxyspirum, fusarium graminearum, bipolaria maydis and fusarium oxgsporum. A liquid bacterial agent prepared from the pseudomonas FRBN3 can be applied in the field of promoting growth of plants and preventing and treating diseases. The liquid bacterial agent can be used in manners of being diluted with water to form spray for use, being blended with seeds or dipped with roots, or being irrigated on root soil.

The invention discloses a bipolaria maydis sporulation medium, and a preparation method and the application of the bipolaria maydis sporulation medium. The bipolaria maydis sporulation medium comprises potatoes, mannitol, agar powder and water, wherein the concentration of the potatoes is 200g / L, the concentration of the mannitol is 5 g / L to 10 g / L, and the concentration of the agar powder is 14g / L. Bipolaria maydis is cultivated on the surface of the bipolaria maydis sporulation medium and is cultivated in a 28-DEG C dark case for 6-7 days, a large amount of conidia which are consistent in form, good in germination effect and high in germination rate can be generated on the surface of the bipolaria maydis sporulation medium, the sporulation quantity is about 1.2 to 3.6 times that of an equivalent PDA (Potato, Dextrose and Agar) culture medium, and the germination rate can be up to more than 90 percent. The bipolaria maydis sporulation medium disclosed by the invention is suitable for subculture of germs and can be used in germ fungicide screening and pathogenecity testing.

The invention relates to a phenanthrene compound in junci medulla as well as a preparation method and application of the phenanthrene compound, belonging to the technical field of phytochemistry. Thestructural formula of the compound is represented by a formula (I) (shown in the description). The phenanthrene compound is separated from all grass of the junci medulla for the first time and is determined as the phenanthrene compound by virtue of a magnetic resonance imaging and mass spectrum determination method, and a specific structure of the phenanthrene compound is represented. The phenanthrene compound presents certain antifungal activity for resisting bipolaria maydis and eggplant phytophthora rot, the lowest inhibition concentrations are 50microg / mL, meanwhile, the phenanthrene compound presents certain bacterial activity for resisting bacterium paratyphosum B and drug-resistant staphylococcus aureus, and the lowest inhibition concentrations are 100microg / mL, so that the phenanthrene compound has good application prospects.

The invention relates to a triterpenoid compound in adiantum capillus-veneris, a preparation method of the triterpenoid compound and application of the triterpenoid compound in electronic cigarettes,belonging to the technical field of phytochemistry. The structural formula of the compound is represented by a formula (I) (shown in the description). The compound is separated from the over-ground part of adiantum capillus-veneris for the first time and is determined as the triterpenoid compound by virtue of a magnetic resonance imaging and mass spectroscopy method, and a specific structure of the compound is represented. The compound presents remarkable antifungal activity to Helminthosporium maydis and Alternaria alternate, and the minimum inhibition concentration is 3.125ug / mL-12.5ug / mL. Meanwhile, the compound presents very good antifungal activity to Micrococcus lysodeikticus, Bacterium paratyphosum B and Pseudomonas aeruginosa and has very good application prospects, and the minimuminhibition concentration is 100ug / mL.

The invention discloses a paenibacillus kribbensis metabolite and application thereof in biocontrol. The structural formula of the paenibacillus kribbensis metabolite is shown as a formula (I). The formula (I) is shown in hte description. The active metabolite has high broad-spectrum antifungal activity, particularly remarkable fungistatic activity for plant pathogenic fungi, such as rice sheath blight fungus, corn southern leaf blight fungus and corn northern leaf blight fungus), and can be mass-produced for preventing and controlling plant diseases, and in terms of the prevention and controlof plant fungal diseases, such as rice sheath blight, corn southern leaf blight and corn northern leaf blight and the preparation of corresponding fungus biocontrol agents, the paenibacillus kribbensis metabolite has a good development and application prospect.

The invention discloses Fritillaria wabuensis endophytic fungi WBS003 and application thereof, the Fritillaria wabuensis endophytic fungi WBS003 is obtained by separation from plants live bulbs of lily family fritillary plant Fritillaria wabuensis by tissue block separation and purification technology, by morphological and molecular biological identification, the Fritillaria wabuensis endophytic fungi WBS003 is identified as cryphonectria nectria haematococca. The strain is preserved in CCCCM (China General Microbiological Culture Collection Center) on 25 November 2014, the preservation number is CGMCC NO.10103, and the strain number is WBS003. By liquid fermentation of the Fritillaria wabuensis endophytic fungi WBS003, broad-spectrum antimicrobial activity of the Fritillaria wabuensis endophytic fungi WBS003 on staphylococcus aureus, pseudomonas aeruginosa and other bacteria, corn helminthosporium maydis, tobacco alternaria alternate, Chinese herbaceous peony colletotrichum orbiculare and other pathogenic fungus can be found, the Fritillaria wabuensis endophytic fungi WBS003 is an important microorganism for search of new resources of fritillary plant antibiotic active substances, and provides a new way for screening of natural antimicrobial drugs.

The invention discloses [(6-substituted-pyrimidine-4-oxy)phenyl]-3-methyl methoxyacrylate having activity of inhibiting pathogenic bacteria on crops and relates to the technical filed of biochemistry.The compound has the structure general formula in the specification and has great activity in applications of inhibiting Fusarium oxysporum.f sp. cucumerinum, Cercospora arachidicola Hori, Macrophomakawatsukai, Rhizoctonia cerealis, Bipolaris maydis, Colletetrichum orbicalare, Fusarium fujikuroi and the like.

The invention discloses pyrazole amide compounds and application thereof, relating to the field of agricultural bactericides. The general formula (I) of the compounds is disclosed in the specification, wherein X is oxygen atom or sulfur atom; R1 is hydrogen atom, halogen, C1-C4 alkyl or alkoxy group, or nitro group; and R2 is hydrogen atom, halogen atom, C1-C4 alkyl or alkoxy group, or nitro group. The compounds have simple structure, have favorable inhibiting activity on leaf spot of corn, scab of wheat and many other plant pathogenic fungi, can be used for preventing and treating plant pathogenic fungi, and provide a new application in the field of pesticides.

The invention relates to application of 3-acetyl thiotetronic acid derivatives in bactericides for controlling phytopathogen. 3-acetyl thiotetronic acid is used as a raw material for synthesizing 3-acetyl-4-benzylidene thiotetronic acid, 3-acetyl-5-p-fluoro-benzylidene thiotetronic acid and 3-acetyl-5-p-trifluoromethyl benzylidene thiotetronic acid under different conditions. The bactericidal activities of the three compounds under different concentrations are measured and the corresponding EC50 values are computed. The 3-acetyl thiotetronic acid derivatives have superior activities in inhibiting bacteria of apple rot, leaf spot of corn, wheat scab and fusarium wilt of tomatoes, have the characteristics of easy degradation, low residual and the like as compared with traditional bactericides, have simple and economic synthetic methods, are lead compounds of bactericides of great value, and can be used for controlling apple rot, leaf spot of corn, wheat scab and fusarium wilt of tomatoes.

The invention discloses novel application of an ocotillol type compound as an agricultural fungicide. The compound is used for preventing and controlling agricultural fungal diseases as the fungicide,can effectively prevent and control crop diseases including rice sheath blights, a rape Sclerotinia disease, wheat scab, grape bitter rot and corn southern leaf blights and is particularly stronger in inhibition activity to the corn southern leaf blights. The compound, as the agricultural fungicide prepared by the active ingredients, has a good control effect to the corn southern leaf blights andhas the characteristics of low toxicity, no residue, no pollution and no public nuisance.

The invention provides an efficient disease-resistant authenticated inoculum for corn leaf blight and a preparation method thereof. The inoculum comprises the following raw materials in proportion byweight: 100 g of sorghum, 0.20 to 0.25 g of magnesium sulfate, 0.20 to 0.25 g of sodium phosphate and 15 to 30 g of corn leaves. The preparation method comprises the following steps: (1) screening ofcorn leaf blight virulent bacterial strains; (2) storage of corn leaf blight virulent bacterial strains; (3) preparation of secondary cultures; (4) preparation of the inoculum; and (5) cultivation ofthe inoculum. The inoculum has the advantages that the obtained bacterial strains for authenticating corn leaf blight disease resistance are bacterial strains with high pathogenicity, high purity andhigh activity, and guarantees are provided for authenticating corn disease resistance inoculation; the work efficiency is greatly improved compared with a plate medium; the infection of gnats and infectious microbes is avoided, so that the high-quality inoculum finished product is obtained; the cultivation of the inoculum and a cultivation condition thereof are beneficial for generation of corn leaf blight germ conidium, so that the inoculum is high in spore formation speed, large in spore formation quantity and long in spore formation period.

The invention discloses a non-traditional peptide KQ-11. The amino acid sequence of the peptide KQ-11 is KQRKKILGCTY. A broad-spectrum antifungal activity experiment of the non-traditional peptide KQ-11 shows that the KQ-11 achieves an inhibition effect on fungi mainly through hypha aggregation. The highest inhibition rates of the peptide KQ-11 on the Bipolaris zeicola, Curvularia lunata, Fusarium graminearum and Bipolaris maydis are 60.4 percent, 61.9 percent, 45.1 percent and 39.9 percent respectively.

The invention discloses a peptide KC-19 with antibacterial activity. The amino acid sequence of the peptide KC-19 is KCHLHPLCKGEALELFSVG. A broad-spectrum antifungal activity experiment of the peptide KC-19 shows that the peptide KC-19 achieves an inhibition effect on fungi mainly through hypha aggregation. The highest inhibition rates of the KC-19 on Bipolaris zeicola, Curvularia lunata, Fusarium graminearum are 47.0%, 47.8% and 25.1% respectively.

The invention discloses bacillus subtilis A16, which is preserved in China Center for Type Culture Collection (CCTCC) with CCTCC NO. 209105. The bacterial colony of the Bacillus subtilis A16 is oyster white on a beef extract peptone culture medium, has irregular shape and no luster, is not wet, has irregular edge, has diameter of 0.5-1cm, and has microfold surface, straight rod-shaped thallus andelliptic spore. The invention also discloses a preparation method and application of the bacillus subtilis A16. The bacillus subtilis A16 has broad bacteriostasis, can suppress exserohilum turcicum and helminthosporium maydis, has higher antagonism for exserohilum turcicum bacteria, helminthosporium maydis bacteria, maize curvularia leaf spot fungus, verticillium dahliae kleb, wheat scab pathogen, colletotrichum musae and banana vascular wilt bacteria, and has high prevention cure efficiency, and good safety and development application prospect.

The invention provides a LAMP detection primer of bipolaris maydis and a visualized detection method and application thereof, and belongs to the technical field of crop disease detection and biology.The LAMP detection primer and the visualized detection method are specially used for specified detection of the bipolaris maydis. The LAMP detection primer of the bipolaris maydis is designed, the primer comprises outer primers F3 and B3, and inner primers FIP and BIP, and the outer and inner primers are as shown in a sequence table. According to the visualized detection method established based on the primer, green fluorescence or ladder shape presented with LAMP characteristics can be observed through chromogenic reaction or agarose gel electrophoresis detection after LAMP isothermal amplification is conducted. By means of the LAMP detection primer and the detection method thereof, rapid, sensitive and accurate detection of the bipolaris maydis can be achieved in production practice, andthe LAMP detection primer and the detection method thereof can be used for the monitoring and identification of pathogenic bacteria and early diagnosis of field diseases to provide reliable technicaland theoretical basis for early warning, prevention and control of the bipolaris maydis.

The invention relates to the technical field of phytoextraction and particularly discloses a myrsine stolonifera extractive, a method for preparing the myrsine stolonifera extractive and application of the myrsine stolonifera extractive. From the perspectives of greening, environmental protection, safety and high efficiency, myrsine stolonifera of plants is used as a raw material of biological pesticide, and the myrsine stolonifera extractive is prepared and extracted through the methyl alcohol chromatography, the resin chromatography and the silica gel chromatography. The myrsine stolonifera extractive has obvious biological inhibitory activities for pests such as houseflies, bipolaris maydism and phyricularia grisea and can be applied to preparation of pesticide preparations.

The invention relates to a cochliobolus heterostrophus ChCDC3 gene and an application thereof, and belongs to the technical field of microorganism genetic engineering. The DNA sequence of the ChCDC3 gene which is from cochliobolus heterostrophus and used for controlling the growth speed of colonies, conidium formation and ascospore formation provided by the invention is as shown in SEQID No:1, andthe amino acid sequence of protein coded by the provided ChCDC3 gene is as shown in SEQID No:2. The ChCDC3 gene can be applied to the genetic engineering field of plants for resisting corn southern leaf blight. The protein ChCDC3 for controlling the growth speed of colonies of the cochliobolus heterostrophus, conidium formation and ascospore formation is subjected to deletion, mutation or modification, the growth of the cochliobolus heterostrophus is slow, the conidium formation and the ascospore formation are limited, lifecycle of the cochliobolus heterostrophus is obstructed, the ChCDC3 gene can be used as a target to be applied to designing and screening corn southern leaf blight resisting medicaments, especially the protein does not exist in plants, and the ChCDC3 gene is safe to theplants.

The invention discloses a preparation method of a soil improvement agent for preventing and treating maize southern leaf blight, and belongs to the field of soil improvement agents. According to the preparation method, a living cicada pupa is first taken; an opening is scratched at the abdomen of the cicada pupa; afterwards, the cicada pupa is put into honey for soaking; after being soaked, the cicada pupa is aired to obtain a solid culture medium; a paecilomyces lilacinus seed solution is uniformly sprinkled on the surface of the solid culture medium to carry out fermentation culture; an obtained substance is dried and ground until lavender downy fungi fully grow, so as to obtain cicada pupa powder covered with paecilomyces lilacinus; subsequently, a waste crab shell is subjected to acid soaking and alkali soaking respectively; after being rinsed and dried, the waste crab shell is mixed with the cicada pupa powder covered with the paecilomyces lilacinus, ammonium dihydrogen phosphate, milk vetch straw, grass carbon and a tea seed cake, so that the soil improvement agent for preventing and treating the maize southern leaf blight can be obtained. The soil improvement agent prepared and obtained by the preparation method can obviously inhibit the growth of a mycelium of helminthosporium maydis, and greatly decreases the morbidity of the maize southern leaf blight; and compared with a conventional chemical insecticide, the soil improvement agent cannot pollute a crop, does not generate drug resistance and is long in prevention and treatment time.

The invention discloses a high-throughput identification method for pathogenic differentiation of bipolaria maydis. The method screens out molecular marker genes related to the pathogenic differentiation mainly according to differences of strains with strong and weak pathogenicity in gene level and transcription level, and the degree of pathogenicity of the strain can be judged by detecting the molecular marker genes of small spot strains. Compared with the prior art, the high-throughput identification method of the invention can identify the degree of the pathogenicity of the bipolaria maydisat a molecular level and on a large sample size, and time and labor are saved.

The invention discloses pyrazole amide compounds and application thereof, relating to the field of agricultural bactericides. The general formula (I) of the compounds is disclosed in the specification, wherein X is oxygen atom or sulfur atom; R1 is hydrogen atom, halogen, C1-C4 alkyl or alkoxy group, or nitro group; and R2 is hydrogen atom, halogen atom, C1-C4 alkyl or alkoxy group, or nitro group. The compounds have simple structure, have favorable inhibiting activity on leaf spot of corn, scab of wheat and many other plant pathogenic fungi, can be used for preventing and treating plant pathogenic fungi, and provide a new application in the field of pesticides.

The invention discloses a methylene diphenyl amide compound and application thereof and relates to the field of agricultural fungicides. The compound has a structure represented by a general formula (I) as shown in the description, wherein X is an oxygen atom or a sulfur atom; and R is a hydrogen atom, a halogen atom, C1-C4 alkyl group or an alkoxy group, or a nitro group. The methylene diphenyl amide compound is simple in structure, and has good inhibitory activity on multiple plant pathogenic fungi such as bipolaris maydis and sclerotinia sclerotiorum. The methylene diphenyl amide compound can be used for preventing and controlling plant pathogenic fungi, which is a novel application of the compound in the field of pesticides.

The invention discloses a formamido diphenyl amide compound and application thereof, and relates to the field of agricultural fungicides. The structure of the compound is showed in the general formula (1), wherein X is an oxygen atom or a sulphur atom; R is a hydrogen atom, a halogen atom, a C1-C4 alkyl group or an alkoxy group. The compounds are simple in structure, have good inhibitory activity for multiple plant pathogenic fungi such as fusarium asiaticum and bipolaris maydis, can be used for preventing and curing the plant pathogenic fungi, and have novel use in the field of pesticides.

The invention discloses a spore-producing medium for corn leaf spot fungus, a preparation method and application thereof. The medium includes potatoes, mannitol, agar powder and water, wherein 200 g / L of potatoes, 5-10 g / L of mannitol, and 14 g / L of agar powder. Cultivated on the surface of the spore-forming medium, and cultivated in the dark at 28°C for 6-7 days, a large number of conidia with uniform shape, good germination effect, and high germination rate can be produced on the surface of the medium, and the sporulation yield is about equal to 1.2 to 3.6 times the sporulation yield of PDA medium, and the germination rate can reach more than 90%. The spore-producing culture medium for the corn leaf spot fungus is suitable for the subculture of the pathogen, and can be used in the screening of the pathogenic agent and the determination of the pathogenicity of the pathogen.

The invention relates to a Cochliobolus heterostrophus ChCDC10 gene and application thereof and belongs to the technical field of microorganism genetic engineering. The DNA sequence of the ChCDC10 gene from Cochliobolus heterostrophus and used for controlling conidium formation, ascospore formation and virulence is as shown in SEQ ID No. 1. The amino acid sequence of protein encoded by the ChCDC10 gene is as shown in SEQ ID NO. 2. The ChCDC10 gene is applicable to the field of plant anti-Cochliobolus heterostrophus genetic engineering. By deleting, mutating or modifying the Cochliobolus heterostrophus protein ChCDC10 for controlling conidium formation, ascospore formation and virulence, conidium formation and ascospore formation are limited, and virulence is lowered; the protein can be used as the target to apply in the design and screening of anti-Cochliobolus heterostrophus chemicals, especially plants do not contain the protein, and plant safety is achieved.