Aniseed essential oil for inhibiting plant pathogenic fungi and preparing method thereof

A technology of plant pathogenic fungi and essential oils, applied in the field of pesticides

Inactive Publication Date: 2008-07-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no evidence that star anise essential oil has any effect on plant pathogenic fungi: Alternaria solani, Botryodiplodiatheobromae, Bipolanis maydis, Fusarium graminearum, Fusarium oxysporum f .

Method used

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  • Aniseed essential oil for inhibiting plant pathogenic fungi and preparing method thereof
  • Aniseed essential oil for inhibiting plant pathogenic fungi and preparing method thereof
  • Aniseed essential oil for inhibiting plant pathogenic fungi and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of star anise essential oil

[0022] The shade-dried star anise fruit is crushed to 40 meshes, an appropriate amount of crushed material is accurately weighed, a certain amount of distilled water is added, and water distillation is carried out after soaking for 1 hour. Add 1 mg of NaCl to every 5 mL of distillate mixed with oil and water, extract with anhydrous ether (repeat 3 times, 10 mL each time), dry with anhydrous sodium sulfate (2 g), let the ether volatilize naturally, and obtain refined The star anise essential oil, sealed and stored at 4 ℃.

[0023] Calculate the oil rate (v / w):

[0024]

Embodiment 2

[0025] Embodiment 2: the chemical composition analysis of star anise essential oil

[0026] GC conditions: the chromatographic column is Agilent HP-5 quartz capillary column [30m×0.25mm×0.25μm (5% phenyl)-methyl polysiloxane]. The temperature of the injection port was 250°C, and the temperature of the transfer line was 240°C. The temperature program is as follows: the initial column temperature is 50°C and stays for 1min; the temperature is raised to 150°C at 3°C / min, and stays for 1min; , stay for 5min. The carrier gas is high-purity helium (99.999%); the column flow rate is 1 mL / min. The essential oil was prepared by the method described in Example 1, and the injection volume of the essential oil was 1.0 μL each time (split ratio: 1:20). The gas chromatogram of star anise essential oil is shown in Figure 1.

[0027] GC-MS analysis: Agilent 6890GC-5973MSD mass spectrometer was used to complete. GC conditions were as described above. MS conditions are as follows: ion sou...

Embodiment 3

[0035] Embodiment 3: the antifungal activity of star anise essential oil and its main component trans-anethole

[0036](1) Medium: potato dextrose agar medium and oat tomato medium

[0037] Potato dextrose agar medium (PDA): The steps for preparing 1L of potato dextrose agar medium (Potato dextrose agar, PDA) are: wash and peel 200g of potatoes, cut into small pieces, add 1000mL of deionized water, boil for 30min , filter, take the filtrate, add 20g of agar and 20g of glucose, heat to melt the agar, filter it with gauze while it is hot, and then add deionized water to make it up to 1000mL. Sterilize with damp heat at 121°C for 20 minutes. This medium is used for the cultivation of fungi other than Magnaporthe oryzae and Pythium melonum.

[0038] Oat tomato agar medium (OTA): The steps to prepare 1L of oat tomato agar medium (Oat-tomato agar medium, OTA) are: chop two fully mature tomatoes, squeeze the juice with a juicer, and pour the juice with four layers of gauze Filter ...

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Abstract

The invention relates to a botanical essential oil for the control of plant pathogenic fungus and a preparation method thereof. The essential oil of the invention is extracted from star aniseseen fruits and contains 29 main ingredients through gas chromatography-mass spectrometry analysis, wherein, trans-anethole accounts for 83.483 percent. The invention first confirms that the trans-anethole is the main antibacterial active ingredient in the essential oil from the star aniseseen as well as the essential oil from the star aniseseen and the trans-anethole thereof has significant inhibition activity for a plurality of plant pathogenic fungus such as early blight of tomato, stem end rot of mango, corn leaf blight, wheat scab, cucumber fusarium wilt, tomato fusarium wilt, cotton fusarium wilt, rice blast fungus, pythium aphani-dermatum, wheat sheath blight, rice sheath blight, etc. and can control plant diseases.

Description

technical field [0001] The invention relates to the preparation and analysis of star anise essential oil with antibacterial activity, the inhibitory effect of star anise essential oil and its main component trans-anethole on plant pathogenic fungi, and belongs to the technical field of pesticides. Background technique [0002] Plant essential oil is a general term for a class of volatile oily liquids present in plants that can be distilled with water vapor and have a certain fragrance. Plant essential oils have various biological activities such as antibacterial and insecticidal, and are widely used in the fields of medicine, pesticides, spices, cosmetics and food. [0003] Star anise (Illicium verum Hook.f) belongs to Magnoliaceae (Magnoliaceae) star anise genus in classification, is an important medicinal plant and spice plant, mainly distributed in my country's Yunnan, Guangxi, Guangdong, Fujian, Anhui, Jiangxi, Hunan and other provinces . my country is the main producer...

Claims

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Application Information

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IPC IPC(8): A01N65/00A01P3/00
Inventor 周立刚郭泽建姜微波黄永富赵江林龚佑文张小娟
Owner CHINA AGRI UNIV
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