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Paenibacillus kribbensis metabolite and application thereof in biocontrol

A technology of Bacillus and biocontrol agents, applied in the field of Paenibacillus Cliburnum metabolites and its application in biocontrol, to achieve remarkable control effect and strong antibacterial effect

Active Publication Date: 2019-01-25
广西科胜生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Paenibacillus mainly focuses on the breeding of excellent strains and improving the production of active substances by means of fermentation or genetic engineering. For the purification of active substances of Paenibacillus kribbensis metabolites , identification and application in the prevention and treatment of plant fungal diseases have not been reported, and there are still great deficiencies and research space

Method used

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  • Paenibacillus kribbensis metabolite and application thereof in biocontrol
  • Paenibacillus kribbensis metabolite and application thereof in biocontrol
  • Paenibacillus kribbensis metabolite and application thereof in biocontrol

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Experimental program
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Effect test

Embodiment 1

[0042] The optimized screening of embodiment 1 Paenibacillus Kliben TRCC82001 substratum

[0043] 1. With sucrose as the main sugar source, NaNO 3 As the main nitrogen source, the culture medium of Paenibacillus Cliburnum metabolites was optimized and screened by orthogonal test method, and the strain growth (concentration of bacterial culture solution, OD 600 ) and measure the antibacterial rate index to rice sheath blight.

[0044] 2. The results are shown in Table 1, the comprehensive sucrose, NaNO 3 、K 2 HPO 4 The impact of these three factors on fermentation at different levels, and finally determine the proportion of each composition of the medium most suitable for the growth of the strain is 3% sucrose, 0.15% NaNO 3 , 0.1% K 2 HPO 4 , the maximum antibacterial rate can reach more than 85%.

[0045] Table 1 Orthogonal experiment design and statistical results of optimization of the distribution ratio of each group of medium

[0046]

[0047]

Embodiment 2

[0048] Example 2 Preparation of Paenibacillus Kliben TRCC82001 metabolites

[0049] 1. Cultivation of Paenibacillus Kliben Metabolites

[0050] Cultivate the metabolites of Paenibacillus Kliben with the above-mentioned optimized and screened basal medium, adjust the pH of the medium to 7-8, and place it under the culture conditions of 30°C and 180r / min, 100mL / 1000mL (v / v) shake flask fermentation culture for 84h.

[0051] 2. Pretreatment of Paenibacillus Cliburnum Metabolites

[0052] After the above-mentioned shaking flask fermentation obtains the fermented liquid, add an equal volume of ethanol, shake vigorously to make the ethanol fully contact with the fermented liquid, and let stand overnight to fully analyze the polysaccharide secreted by the thalline. Remove the precipitated polysaccharides by low-temperature freezing and centrifugation to obtain a clear fermentation liquid, and then use a rotary evaporator to conduct vacuum distillation and concentration to remove e...

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Abstract

The invention discloses a paenibacillus kribbensis metabolite and application thereof in biocontrol. The structural formula of the paenibacillus kribbensis metabolite is shown as a formula (I). The formula (I) is shown in hte description. The active metabolite has high broad-spectrum antifungal activity, particularly remarkable fungistatic activity for plant pathogenic fungi, such as rice sheath blight fungus, corn southern leaf blight fungus and corn northern leaf blight fungus), and can be mass-produced for preventing and controlling plant diseases, and in terms of the prevention and controlof plant fungal diseases, such as rice sheath blight, corn southern leaf blight and corn northern leaf blight and the preparation of corresponding fungus biocontrol agents, the paenibacillus kribbensis metabolite has a good development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological control, and more specifically relates to a metabolite of Paenibacillus kribben and its application in biological control. Background technique [0002] The Food and Agriculture Organization of the United Nations (FAO) survey and statistics show that the annual loss of world food due to weed damage reaches 11%, the loss due to rodent damage reaches 20%, the loss due to pest damage reaches 14%, and the loss due to disease damage reaches 10%. Chemical pesticides are the main measures to solve diseases, insects, weeds, and rodents in the process of food production. However, with the excessive use of chemical pesticides, they have adverse effects on environmental ecology, soil structure, and microbial diversity. In addition, plant pathogenic bacteria Severe resistance has developed to many varieties, and the use of chemical pesticides has become more and more restricted. At present, my country is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04A01P3/00C12P17/18C12R1/01
CPCC07D487/04C12P17/182
Inventor 廖美德舒灿伟胡亮亮赵鹏飞郑文博
Owner 广西科胜生物科技有限公司
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