Bipolaria maydis sporulation medium, and preparation method and application of bipolaria maydis sporulation medium

A technology of corn spot disease bacteria and culture medium, applied in the field of plant pathogenic fungus research, can solve problems such as complex operation process, achieve good growth, reduce unnecessary links, and simple production method

Active Publication Date: 2015-10-07
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the water agar plate surface germination method to carry out fungicide inhibition of pathogenic fungus spore germination test, usually requires a corresponding concentration

Method used

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  • Bipolaria maydis sporulation medium, and preparation method and application of bipolaria maydis sporulation medium
  • Bipolaria maydis sporulation medium, and preparation method and application of bipolaria maydis sporulation medium
  • Bipolaria maydis sporulation medium, and preparation method and application of bipolaria maydis sporulation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the influence of different mediums on the growth of pathogen mycelium, sporulation

[0026] Tested strain: corn leaf spot fungus FJ.

[0027] Test medium: mannitol medium, potato dextrose medium (PDA medium), potato sucrose medium (PSA medium) and 1.4% water agar medium.

[0028] Wherein the preparation method of mannitol medium is as follows:

[0029] Weigh and wash the potatoes, cut them into small pieces, add water to boil for 0.5h, filter the potatoes with gauze, add water to make up to 1L, divide them into Erlenmeyer flasks, then add mannitol and agar powder in proportion, sterilize at 121°C for 25min, and wait for the culture medium to cool At about 50°C, pour into a petri dish with a diameter of 8.9cm, about 25ml per dish, and set aside. The quality of raw materials for preparing different concentrations of mannitol medium is shown in Table 1:

[0030] The quality of each raw material of table 1 mannitol medium

[0031]

Potatoes (...

Embodiment 2

[0043] Example 2: Application of spores cultivated in mannitol medium in drug screening

[0044] Tested strain: corn leaf spot fungus FJ.

[0045] The test agents: pyraclostrobin technical (BASF, Germany), iprodione technical (Jiangsu Huifeng Co., Ltd.) and fluazinam technical (Lier Chemical Co., Ltd.).

[0046] Test medium: medium containing 0.5% mannitol and 1.4% water agar medium.

[0047] Preparation of drug-containing medium: On the basis of preliminary experiments, water agar media with different concentrations of drugs in Table 4 were prepared respectively, and the medium without drug was used as a control, and repeated 3 times (3 dishes).

[0048] Test method: use 0.5% mannitol medium to cultivate the pathogenic bacteria for 6-7 days, then cover the surface of the culture medium with the same bacterial age with a sterile filter paper square (about 1.5×1.5cm in size), after the filter paper is wet, Move the filter paper to the surface of the water agar medium containi...

Embodiment 3

[0054] Embodiment 3: the application of the spore cultivated in mannitol medium in pathogenicity determination of pathogen

[0055] Tested strain: Corn spot fungus FJ

[0056] Tested corn variety: T1402

[0057] Test medium: 0.5% mannitol medium, 1.0% mannitol medium, PDA medium and PSA medium.

[0058] Test method: After the fungus has been activated, use an inoculation needle to inoculate the germ cake into the center of the medium plate, with the mycelium facing down, and culture in the dark at 28°C for 7 days (repeat this operation for subculture) to obtain a large number of meristems After spores, inject clean water on the surface of the culture medium, scrape and wash the spores on the surface of the culture medium with a sterile glass slide, filter with double-layer sterile gauze, and dilute the spore suspension with clean water to make the final concentration of conidia 1-10× 10 5 per mL, spray the spore suspension on corn plants (5-6 leaf stage), cover the inoculat...

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Abstract

The invention discloses a bipolaria maydis sporulation medium, and a preparation method and the application of the bipolaria maydis sporulation medium. The bipolaria maydis sporulation medium comprises potatoes, mannitol, agar powder and water, wherein the concentration of the potatoes is 200g/L, the concentration of the mannitol is 5 g/L to 10 g/L, and the concentration of the agar powder is 14g/L. Bipolaria maydis is cultivated on the surface of the bipolaria maydis sporulation medium and is cultivated in a 28-DEG C dark case for 6-7 days, a large amount of conidia which are consistent in form, good in germination effect and high in germination rate can be generated on the surface of the bipolaria maydis sporulation medium, the sporulation quantity is about 1.2 to 3.6 times that of an equivalent PDA (Potato, Dextrose and Agar) culture medium, and the germination rate can be up to more than 90 percent. The bipolaria maydis sporulation medium disclosed by the invention is suitable for subculture of germs and can be used in germ fungicide screening and pathogenecity testing.

Description

technical field [0001] The invention relates to the technical field of research on plant pathogenic fungi, in particular to a spore-producing medium for spore-forming fungi of maize spot and its preparation method and application. Background technique [0002] Southern corn leaf blight is an important disease in corn production, widely distributed in my country. Corn blight is an airborne disease. The conidia produced by P. maize play an important role in the spread, infection and pathogenicity of the pathogen. The conidia can be spread to the corn leaves growing in the field by means of airflow or rain. When there are free water droplets, the conidia The spores can germinate in 4-8 hours to produce germ tubes and invade the epidermal cells of corn leaves, and lesions can be formed within 3-4 days, and then the lesions can produce conidia, which spread with the help of airflow and repeatedly infect corn grown in the field . At present, using the disease resistance of crop...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12N1/14C12Q1/04C12R1/645
Inventor 杨秀娟陈福如甘林阮宏椿杜宜新石妞妞
Owner INST OF PLANT PROTECTION FAAS
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