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Novel application of ocotillol type compound as agricultural fungicide

A compound and fungicide technology, applied in the agricultural field, can solve the problems of no agricultural fungicide and less active research, and achieve the effects of excellent bactericidal activity, favorable environmental protection, and broad antibacterial spectrum.

Active Publication Date: 2018-07-13
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on its activity, and so far there are no relevant literature or reports on the application of agricultural fungicides in agriculture.

Method used

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  • Novel application of ocotillol type compound as agricultural fungicide
  • Novel application of ocotillol type compound as agricultural fungicide
  • Novel application of ocotillol type compound as agricultural fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Ocotillol type compound is to the control of agricultural disease

[0033] The tested strains Helminthosporium maydis Nisik&Miy were isolated from diseased leaves of corn; Rhizoctonia solani (Thanatephorus cucumeris) were isolated from diseased leaves of rice sheath blight; Colletotrichum gloeosporioides was isolated from diseased fruit of grapes; Fusarium graminearum was isolated from ears of wheat head blight.

[0034] Dissolve the test compounds in methanol respectively to make 1000 μg / mL mother solution, and store them at 4°C for later use. PDA medium: cut 200g of peeled potatoes into pieces, boil for 10min, take the juice, add 20g of agar, heat until melted, then add 20g of glucose, and dilute to 1000mL with water.

[0035] The inhibition of mycelium growth was measured by the mycelium growth rate assay method, and the bacteria dish (5 mm in diameter) was taken from the edge of the pre-cultivated test bacteria on the PDA plate, and the mother liquor ...

Embodiment 2

[0040] Since the compound has a good control effect on the fungal disease maize leaf spot, the inhibition of the compound on mycelial growth was determined by the method of measuring mycelial growth rate. Take the plate (diameter 5mm) from the edge of the pre-cultured test bacteria on the PDA plate, mix the mother solution of the test agent with the medium evenly, so that the final concentration of the test agent is 30, 20, 10, 5, 2.5ppm, mix The solution was poured into a sterilized petri dish with a diameter of 9 cm to make a plate, and each plate was inoculated with a fungus plate with the mycelium facing upward, and each treatment was repeated 3 times. Cultivate at 25°C until the control colonies cover more than two-thirds of the petri dish, and measure the growth diameter of the colonies by the cross method. According to the following formula, the inhibition rate (%) at different concentrations was calculated. Inhibition rate (%)=[(control colony diameter (mm)-treatment ...

Embodiment 3

[0045] Compound 1 (or compound 2, or compound 13, or compound 14) was weighed, and conventional excipients such as lignin and diatomaceous earth were used to prepare a wettable powder with an active ingredient concentration of 2% according to the conventional process.

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PUM

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Abstract

The invention discloses novel application of an ocotillol type compound as an agricultural fungicide. The compound is used for preventing and controlling agricultural fungal diseases as the fungicide,can effectively prevent and control crop diseases including rice sheath blights, a rape Sclerotinia disease, wheat scab, grape bitter rot and corn southern leaf blights and is particularly stronger in inhibition activity to the corn southern leaf blights. The compound, as the agricultural fungicide prepared by the active ingredients, has a good control effect to the corn southern leaf blights andhas the characteristics of low toxicity, no residue, no pollution and no public nuisance.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a new application of an ocotilol compound as an active ingredient to prepare an agricultural fungicide. Background technique [0002] Crop diseases are not as easy to be detected in time as pests and weeds, so they often cause neglect and difficulty in prevention and control, so their harm is even more serious. According to reports, there are as many as 10,000 plant diseases caused by pathogenic fungi all over the world, and the losses caused account for 10% to 30% of the total annual crop losses. If plant diseases caused by viruses, bacteria and nematodes are included, the losses will be even more considerable. [0003] In order to meet people's increasing demand for food and other living materials, chemicals have to be used to protect crops from harmful organisms, among which fungicides play an important role. At present, commonly used chemical agents such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/08A01N43/16A01N43/653A01P3/00C07J17/00C07H15/256
CPCA01N43/08A01N43/16A01N43/653C07H15/256C07J17/00C07J17/005
Inventor 孟庆国宋萍萍王文智张建强王朝明刘智邓建强王嘉珍翁卫钊何萌
Owner YANTAI UNIV
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