Antagonistic and growth-promoting pseudomonas producing ACC deaminase and application thereof

A Pseudomonas, growth-promoting technology, applied in applications, plant growth regulators, plant growth regulators, etc., can solve the problems of crop yield reduction, quality decline, etc., to improve competition for iron nutrition, promote watermelon growth, Aids in the absorption of iron nutrients

Inactive Publication Date: 2015-08-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Plant diseases and insect pests, pesticide residue pollution, heavy metal pollution, high temperature, drought, waterlogging, frost and other adversity environments have seriously reduced the yield and quality of crops

Method used

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  • Antagonistic and growth-promoting pseudomonas producing ACC deaminase and application thereof
  • Antagonistic and growth-promoting pseudomonas producing ACC deaminase and application thereof
  • Antagonistic and growth-promoting pseudomonas producing ACC deaminase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A growth-promoting and disease-preventing bacterial strain FRBN3 capable of producing ACC deaminase and antagonizing various plant pathogenic fungi of the present invention is obtained by separating and purifying from the root surface of watermelon plants growing robustly at Anhui Agricultural University in Hefei City, Anhui Province. The specific separation, The screening method is as follows:

[0024] 1 Isolation of strains

[0025] Shake the watermelon roots to remove a large amount of soil attached to the root system, then soak 10 grams of watermelon roots with a small amount of surface soil into a triangular flask filled with 90mL of sterile water, shaker 160r min -1 Shake for 30 minutes to make a sample suspension, and use a 10-fold gradient dilution method to dilute to 10 -7 , take 0.1mL of the appropriate dilution of the suspension and spread it on the beef extract peptone medium plate, after culturing at 28°C for 3 days, pick single colonies with different col...

Embodiment 2

[0039] The siderophore-producing ability of the strain was determined according to Wang Pingping's method. The strain FRBN3 was inoculated in MKB liquid medium (casamino acid 5.0g; glycerol 15mL; K 2 HPO 4 2.5g; MgSO 4 ·7H 2 O 2.5g; distilled water 1000mL; pH 7.2), 160r min -1 Incubate at 28°C for 48h. The cultured bacteria liquid was 8000r·min -1 Centrifuge for 10 min, take 2 mL of the supernatant and add it to an equal volume of CAS detection solution, mix well and react for 1 h, use double distilled water as a control to adjust to zero, and measure the absorbance at 630 nm wavelength (denoted as A). At the same time, take 2mL of CAS detection solution and 2mL of uninoculated MKB liquid medium supernatant and mix well, and measure the absorbance value in the same way as the reference value (denoted as Ar). The value of A / Ar<1 indicates that the strain can produce siderophore, and the smaller the value of A / Ar, the greater the production of siderophore. The general ev...

Embodiment 3

[0043] The isolated and screened strain FRBN3 capable of producing ACC deaminase and antagonizing various plant pathogenic bacteria was identified by the following methods:

[0044] 1 Morphological characteristics

[0045] The obtained strain FRBN3 with ACC deaminase activity was streaked on beef extract peptone medium, cultured for 2 days, and described according to the characteristics of bacterial colonies. Obtained its morphological characteristics are as follows: colony light yellowish white, wet, smooth surface, opaque, neat edges.

[0046] The bacterial strains grown to the logarithmic phase were observed under an optical microscope by the Gram staining method, and it was found that the bacterial body was rod-shaped, without spores, and the Gram staining was negative.

[0047] 2 Physiological and biochemical characteristics

[0048] The physiological and biochemical characteristics of the above isolated strains were measured referring to the "Common Bacterial System Id...

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Abstract

The invention relates to an antagonistic and growth-promoting pseudomonas FRBN3 producing ACC deaminase and an application thereof. The pseudomonas FRBN3 is obtained through separation on surface of watermelon roots from Hefei city, Anhui province and is named Pseudomonas guariconensis. The pseudomonas FRBN3 is preserved in China General Microbiological Culture Collection Center at Apr.14 2015 and is assigned the accession number CGMCC No.10715. The pseudomonas FRBN3 has an ACC deaminase activity and can highly produce siderophores. The pseudomonas FRBN3 has a certain inhibition effect on all of fusarium oxyspirum, fusarium graminearum, bipolaria maydis and fusarium oxgsporum. A liquid bacterial agent prepared from the pseudomonas FRBN3 can be applied in the field of promoting growth of plants and preventing and treating diseases. The liquid bacterial agent can be used in manners of being diluted with water to form spray for use, being blended with seeds or dipped with roots, or being irrigated on root soil.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and in particular relates to an antagonistic Pseudomonas bacterial strain FRBN3 producing ACC deaminase and its application in promoting plant growth and preventing and controlling plant diseases. Background technique [0002] Watermelon Fusarium wilt is a major fungal soil-borne disease in watermelon production. This disease can occur in various growth stages of watermelon. The annual incidence rate is 10%-30% in general, and it can reach more than 50% in severe cases. It seriously affects the growth and development of watermelon. Yield. Fusarium oxysporum f.sp.niveum (Fusarium oxysporum f.sp.niveum) is the main pathogen causing watermelon wilt. It can survive in the soil for about 10 years and is the most destructive soil-borne pathogen. One of the plant pathogenic microorganisms. At present, rootstock grafting and application of chemical pesticides are the main contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/38
Inventor 孙乐妮唐欣昀李清杨恩东陈晓琳曹媛媛常艳赵烨
Owner ANHUI AGRICULTURAL UNIVERSITY
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