Fritillaria wabuensis endophytic fungi WBS003 and application thereof
A technology of endophytic fungi and Fritillaria, applied in the field of microorganisms, can solve the problems of long growth period, failure to meet market needs, low total output, etc., and achieve the effect of great application value
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[0023] In the embodiment of the present invention, an endophytic fungus WBS003 of Fritillaria wabu and its application.
[0024] 1. Isolation and purification of strains:
[0025] The endophytic fungus WBS003 of Wabu Fritillaria of the present invention is a bacterial strain obtained from healthy bulbs of Wabu Fritillaria planted in Songpinggou Wabu Fritillaria Base of Maoxian County. The endophytic fungus WBS003 of Wabu Fritillaria of the present invention is separated and obtained by the following steps: rinse with water for 3 to 4 times → rinse with 75% alcohol for 30 s → Rinse with sterile water for 3 to 5 times → sterilize with 0.1% mercuric chloride for 3 to 5 minutes → rinse with sterile water for 3 times. The material is cultured on a plate of PDA medium by the three-print method, and cultured in the dark in a 28°C incubator. After 3 to 4 days, observe whether there are colonies. If no colonies are found in the PDA medium, it proves that the surface of the material is...
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