Fritillaria wabuensis endophytic fungi WBS003 and application thereof

A technology of endophytic fungi and Fritillaria, applied in the field of microorganisms, can solve the problems of long growth period, failure to meet market needs, low total output, etc., and achieve the effect of great application value

Active Publication Date: 2015-07-15
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wabu Fritillaria mostly grows on the western Sichuan Plateau at an altitude of 3000-4000m, and is mainly produced in Ganzi Prefecture,

Method used

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  • Fritillaria wabuensis endophytic fungi WBS003 and application thereof
  • Fritillaria wabuensis endophytic fungi WBS003 and application thereof
  • Fritillaria wabuensis endophytic fungi WBS003 and application thereof

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Embodiment 1

[0023] In the embodiment of the present invention, an endophytic fungus WBS003 of Fritillaria wabu and its application.

[0024] 1. Isolation and purification of strains:

[0025] The endophytic fungus WBS003 of Wabu Fritillaria of the present invention is a bacterial strain obtained from healthy bulbs of Wabu Fritillaria planted in Songpinggou Wabu Fritillaria Base of Maoxian County. The endophytic fungus WBS003 of Wabu Fritillaria of the present invention is separated and obtained by the following steps: rinse with water for 3 to 4 times → rinse with 75% alcohol for 30 s → Rinse with sterile water for 3 to 5 times → sterilize with 0.1% mercuric chloride for 3 to 5 minutes → rinse with sterile water for 3 times. The material is cultured on a plate of PDA medium by the three-print method, and cultured in the dark in a 28°C incubator. After 3 to 4 days, observe whether there are colonies. If no colonies are found in the PDA medium, it proves that the surface of the material is...

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Abstract

The invention discloses Fritillaria wabuensis endophytic fungi WBS003 and application thereof, the Fritillaria wabuensis endophytic fungi WBS003 is obtained by separation from plants live bulbs of lily family fritillary plant Fritillaria wabuensis by tissue block separation and purification technology, by morphological and molecular biological identification, the Fritillaria wabuensis endophytic fungi WBS003 is identified as cryphonectria nectria haematococca. The strain is preserved in CCCCM (China General Microbiological Culture Collection Center) on 25 November 2014, the preservation number is CGMCC NO.10103, and the strain number is WBS003. By liquid fermentation of the Fritillaria wabuensis endophytic fungi WBS003, broad-spectrum antimicrobial activity of the Fritillaria wabuensis endophytic fungi WBS003 on staphylococcus aureus, pseudomonas aeruginosa and other bacteria, corn helminthosporium maydis, tobacco alternaria alternate, Chinese herbaceous peony colletotrichum orbiculare and other pathogenic fungus can be found, the Fritillaria wabuensis endophytic fungi WBS003 is an important microorganism for search of new resources of fritillary plant antibiotic active substances, and provides a new way for screening of natural antimicrobial drugs.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus WBS003 of Wabu Fritillaria and its application. Background technique [0002] Wabu Fritillaria (F.unibracteata Hsiao et K.C.Hsiavar.wabuensis (S.Y.Tang et S.C.Yue) Z.D.Liu, S.Wang et S.C.Chen) is a plant of the genus Fritillaria in the family Liliaceae, which was included in the 2010 edition of "Chinese Pharmacopoeia" as Sichuan One of the base species of Fritillaria medicinal materials. Its bulbs are used as medicine, which has the effects of clearing away heat and nourishing the lungs, resolving phlegm and relieving cough. Wabu Fritillaria mostly grows on the western Sichuan plateau at an altitude of 3000-4000m, and is mainly produced in Ganzi Prefecture, Sichuan Province. It has a long growth period and its total output is relatively low, which is far from meeting the needs of the market. Fritillaria has pharmacological effects such as antitussive...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P1/00A01P3/00C12R1/645
CPCC12N1/145C12R2001/645
Inventor 吴卫苏雪梅潘峰陈艾萌唐小琴
Owner SICHUAN AGRI UNIV
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