Cystis coronis ls1 strain

A technology of Saccharomyces coronoides and strains, applied in the field of microorganisms, can solve the problems of chemical fungicides that are not easy to decompose, pesticide residues that endanger human health, and environmental pollution.

Active Publication Date: 2019-11-26
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chemical fungicides are not easy to decompose, and long-term use will easily cause environmental pollution, cause plant pathogenic bacteria to develop drug resistance, and their pesticide residues will endanger human health

Method used

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  • Cystis coronis ls1 strain
  • Cystis coronis ls1 strain
  • Cystis coronis ls1 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Fuzhuan tea made from tea leaves collected in Qianyang, Hunan, has a "flowering phenomenon". Pick the golden-yellow occluded shell on Fuzhuan tea and inoculate it on the PDA medium, culture at 28°C for 5 days, pick the hyphae for streaking the plate, repeat the purification three times to obtain the strain LS1 of the present invention.

Embodiment 2

[0015] Preparation of the spore suspension: the spore suspension of the strain LS1 of the present invention was prepared with the existing technology, and the concentration was 3×10 7 Pieces / mL, to be used.

[0016] The preparation of fermentation broth: the absorption concentration is 3×10 7 The above-mentioned spore suspension was inoculated with 1% pueraria lobata fermentation medium (2.5 g pueraria lobata and 10 mL water) with the above-mentioned spore suspension liquid per mL, and cultured at a constant temperature of 28°C. After 7 days, the fermentation product was ultrasonically extracted with 95% mass concentration of ethanol, and the supernatant was taken and evaporated to a constant weight by a rotary evaporator to obtain a fermentation product. The fermentation product was diluted with distilled water to a mass concentration of 0.1 mg / mL.

[0017] Preparation of unfermented broth: Pueraria lobata was crushed into powder and diluted to 25mL with distilled water. The mass ...

Embodiment 3

[0019] Example 3 Inhibition of pathogenic fungi

[0020] The tested pathogenic fungi: Colletotrichum (Colletotrichum), Tomato Early Blight (Alternariasonali), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Alternaria brassicae (Alternaria brassicae) were provided by the Microbiology Laboratory of the College of Food Science and Technology, Hunan Agricultural University.

[0021] The chemical fungicide hymexazol: purchased from Zhengzhou Yukang Biotechnology Co., Ltd., with an active ingredient content of 80.1%; hymexazol was diluted 1000 times with sterile water as a positive control.

[0022] Treatment group (pueraria lobata fermentation broth, pueraria unfermented broth) and positive control group: using the plate confrontation method, first pour 5mL PDA into a petri dish with a diameter of 90mm, then add 2mL treatment group or hymexazol solution after solidification, shake After homogenization, add 15mL PDA. The medium of the blank control group was added with 2 mL of steri...

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Abstract

A strain of Eurotium Crisatum LS1, which is classified as Eurotium Crisatum LS1, was preserved in the China Center for Type Culture Collection on June 17, 2015, and the preservation number is CCTCC NO: M 2015379. The strain LS1 can grow and biotransform on kudzu root, and the kudzu root is fermented by using the strain LS1 of the present invention, and the obtained fermentation product shows stronger inhibition on bean anthracnose, tomato early blight, rape sclerotia and cabbage black spot The inhibitory effect on Staphylococcus aureus, Bacillus cereus, Escherichia coli, Bacillus subtilis, and Salmonella, which are five spoilage-causing bacteria, is all improved compared with that before fermentation. It shows that the product of Pueraria lobata fermented by bacterial strain LS1 of the present invention has broad-spectrum antibacterial activity, can inhibit pathogenic fungi and spoilage bacteria to a certain extent, and is expected to be developed into a biological pesticide to prevent and treat crop diseases, and is expected to be used as a food fungicide for food preservation.

Description

Technical field [0001] The invention belongs to the field of microorganisms and relates to a Eurotium Cristatum LS1 strain. Background technique [0002] Food is susceptible to microbial contamination in all aspects of raw material production, post-harvest, production, packaging, storage, transportation and sales. For a long time, refrigeration, freezing, pickling, radiation, chemical fungicides, etc. have been used to prevent food from spoiling and spoiling. Among them, chemical fungicides have been widely used due to their low cost, simple operation, and obvious effects. However, chemical fungicides are not easy to decompose, and long-term use can easily cause environmental pollution, cause plant pathogenic bacteria to develop drug resistance, and their pesticide residues endanger human health. Due to the inherent shortcomings and unreasonable use of chemical fungicides, people have realized the potential hazards of chemical fungicides, and began to pay attention to the develo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 易有金王璐夏菠曹熙胡谢欣
Owner HUNAN AGRICULTURAL UNIV
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