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A strain of Penicillium citricaria with the function of preventing disease and promoting growth and its bacterial agent and application

A technology for Penicillium tangerineum and Penicillium tangerineum is applied in the field of microorganisms to achieve the effects of strong growth-promoting ability, growth promotion and reduction of morbidity rates

Active Publication Date: 2022-07-15
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The reports on Penicillium citrinum mainly use it as a fermentation strain. Studies have found that Penicillium citrinum is the dominant strain producing lipase and the dominant population producing α-amylase in the fermentation process of soybean paste. A report on the systematic research on the effect of mildew on growth promotion and disease prevention and its application

Method used

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  • A strain of Penicillium citricaria with the function of preventing disease and promoting growth and its bacterial agent and application
  • A strain of Penicillium citricaria with the function of preventing disease and promoting growth and its bacterial agent and application
  • A strain of Penicillium citricaria with the function of preventing disease and promoting growth and its bacterial agent and application

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Embodiment 1, the separation method of bacterial strain 44M-3

[0054] Penicillium 44M-3 was isolated from soil samples in Yutong Village, Mianchi County, Sanmenxia City, Henan Province.

[0055] Separation method: dry the soil samples at room temperature, grind and sieve them, weigh 10 g of the ground and sieved soil samples, add 90 mL of sterile water and fully shake to obtain a soil suspension, which is 10 of the soil samples. -1 , and then the soil suspension was shaken, and then the gradient was diluted to 10 -2 , 10 -3 , 10 -4 and 10 -5 , take 100 μL of dilutions of different gradients and spread them on red Bengal medium, invert in a constant temperature incubator at 28°C for 48h, until the colonies grow, pick a single colony and cultivate it on PDA medium for 5d, pick the edge of the colony. The mycelial block was transferred to a new PDA plate, and the transfer was repeated 3 times to obtain a pure culture of the isolate.

Embodiment 2

[0056] Example 2. Inhibitory effect of strain 44M-3 on 14 pathogenic fungi on 5 plants

[0057] Plate co-cultivation method: inoculate the strain 44M-3 on the PDA medium plate for 8-10 days, when 44M-3 produces a large number of conidia, elute the spores with 0.05% (v / v) Tween 80 solution, and prepare the spores Suspension (2×10 7 CFU / mL);

[0058] Coat the PDA plate with 44M-3 spore suspension. After culturing at 28°C for 12h, punch the bacterial cake with a 6mm diameter hole punch and transfer it to a new PDA plate at a symmetrical position of 0.5mm from the edge, in the center of the plate. Inoculated with pathogenic bacteria, the pathogenic bacteria are 6 pathogenic bacteria on sesame seeds, Fusarium oxysporum, Fusarium solani, Helminthosporium soxhlet, Coccidioides bean, Rhizoctonia solani, Corynebacterium multimaster and Coccidioides spp. 4 strains of pathogenic bacteria on tobacco, Conchlea, Phytophthora, Fusarium oxysporum and Alternaria, Rhizoctonia graminearum mol...

Embodiment 3

[0064] Embodiment 3, the preparation of strain 44M-3 high sporopollen

[0065] Inoculate Penicillium citricaria on PDA medium, incubate alternately between light and dark for 5-7 days in a constant temperature incubator at 25°C, 8h light / 16h dark, and wash with 0.05% (v / v) Tween 80 sterile water. Desporulate to form spore suspension; divide PDB into 300mL conical flasks, each bottle is 200mL, and each bottle is connected to 5mL spore suspension, 180r / min, 28 ℃ shaker culture for 4d, as the seed liquid of solid fermentation culture.

[0066] Weigh 1500 g of wheat, soak it in distilled water for 8 hours, remove the water to control it, and divide it into fungal culture bags, 100 g per bag, sterilize at 121°C for 30 minutes. Each bag was inoculated with 10 mL of seed solution, and the seed solution was evenly infiltrated by the wheat, placed in a 30°C dark constant temperature incubator for 7-10 days. When the solid fermentation medium is fully sporulated, the solid fermented pr...

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Abstract

The present invention relates to a strain of Penicillium tangerineum with growth-promoting effect and broad-spectrum disease prevention effect and a fungicide thereof. Culture Collection Center, deposit number: CCTCC NO: M 2019768. Using the strain of Penicillium citricaria to prepare a high-sporopollen fungicide, when using, resuspend the high-sporopollen with 0.05% (v / v) Tween 80 sterile water, and treat the plants by dipping or irrigating the roots with the spore suspensions of different concentrations. root. The Penicillium citricariae of the invention has a good inhibitory effect on pathogenic fungi on various crops; the Penicillium citricarius high sporopollen suspension can promote the growth of corn, wheat, cotton, sesame, cucumber and tobacco roots and plants At the same time, the bacterial agent has good biological safety, and the biological agent will not cause common problems of chemical pesticides such as drug resistance, pesticide residues, and environmental pollution. It is healthy for humans and animals, environmentally friendly, and meets the needs of ecological agriculture and green environmental protection.

Description

technical field [0001] The invention relates to a strain of Penicillium citricariae capable of preventing and treating various diseases and having a growth-promoting effect and an application thereof, belonging to the technical field of microorganisms. Background technique [0002] my country has long relied on chemical pesticides for crop disease prevention and control, resulting in excessive use of pesticides, which may cause environmental pollution and food pesticide residues, and bring potential harm to human health. The use of microbial preparations and their metabolites that are harmless to the human body and the ecological environment can reduce the application of chemical pesticides, which has become a worldwide development direction. Biopesticides are favored in controlling the occurrence and spread of diseases because of their environmental friendliness, safety for humans and animals, long-term effects, and no residues. [0003] At present, the most studied biocon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G13/00A01G22/00A01G22/20A01G22/50A01G22/05A01G22/45A01G22/25A01G17/00A01N63/36A01P3/00A01P1/00A01P21/00C12R1/80
CPCC12N1/14A01G13/00A01G22/00A01G22/20A01G22/50A01G22/05A01G22/45A01G22/25A01G17/005C12R2001/80C12N1/145
Inventor 刘新涛赵新贝倪云霞赵辉刘红彦千慧敏何碧珀王婧张春艳
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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