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Kit for low-energy laser induction of vascularization of mesenchymal stem cells

A stem cell and laser-induced technology, applied in the field of cells, can solve the problems of low efficiency and unsatisfactory effect, and achieve the effect of high efficiency, good effect of induced differentiation, and high separation purity

Active Publication Date: 2017-08-15
华夏(青岛)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of this method to induce differentiation is low, and the effect is not ideal, and it cannot avoid problems such as hemangioma caused by excessive vascularization during the induction of differentiation.

Method used

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  • Kit for low-energy laser induction of vascularization of mesenchymal stem cells
  • Kit for low-energy laser induction of vascularization of mesenchymal stem cells
  • Kit for low-energy laser induction of vascularization of mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A kit for low-energy laser-induced vascularization of mesenchymal stem cells, comprising a box body, a red-light laser-based low-energy laser rehabilitation treatment instrument inside the box body, and several reagent bottles, each of which contains Tissue decomposition fluid, cell culture fluid and cell digestion fluid. The cell culture fluid is mainly formed by dissolving α-MEM medium, fetal bovine serum, penicillin, streptomycin, and ethyl maltol with water for injection. Dissolve the α-MEM medium 30g, the fetal calf serum 3g, the penicillin 1×10 in the water for injection 5 U, the streptomycin 0.1g, the ethyl maltol 1g.

Embodiment 2

[0048] A kit for low-energy laser-induced vascularization of mesenchymal stem cells, comprising a box body, a red-light laser-based low-energy laser rehabilitation treatment instrument inside the box body, and several reagent bottles, each of which contains Tissue decomposition fluid, cell culture fluid and cell digestion fluid. The cell culture fluid is mainly formed by dissolving α-MEM medium, fetal bovine serum, penicillin, streptomycin, and ethyl maltol with water for injection. Dissolve the α-MEM medium 50g, the fetal calf serum 8g, the penicillin 3×10 in the water for injection 5 U, the streptomycin 0.3g, the ethyl maltol 3g.

Embodiment 3

[0050] A kit for low-energy laser-induced vascularization of mesenchymal stem cells, comprising a box body, a red-light laser-based low-energy laser rehabilitation treatment instrument inside the box body, and several reagent bottles, each of which contains Tissue decomposition fluid, cell culture fluid and cell digestion fluid. The cell culture fluid is mainly formed by dissolving α-MEM medium, fetal bovine serum, penicillin, streptomycin, and ethyl maltol with water for injection. Dissolve the α-MEM medium 40g, the fetal calf serum 5g, the penicillin 2×10 in the water for injection 5 U, the streptomycin 0.2g, the ethyl maltol 2g.

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Abstract

The present invention provides a kit for low-energy laser induction of vascularization of mesenchymal stem cells. The kit comprises a kit body, a red laser-based low-energy laser rehabilitation treatment instrument positioned in the kit body, and a plurality of reagent bottles, and the plurality of reagent bottles respectively contain a tissue decomposition solution, a cell culture solution and a cell digestion solution. The kit for low-energy laser induction of vascularization of mesenchymal stem cells separates mesenchymal stem cells from tissues, carries out culture and amplification, and vascularizes the mesenchymal stem cells under the induction of low energy laser, so the kit has the advantages of high separation purity, good culture proliferation effect, good induction differentiation effect, high efficiency, effective prevention of the excess vascularization problem in the induction process, obtaining of abundant vascular endothelial cells used for promoting post-traumatic early skin vascularization, and facilitation of promotion of traumatic skin restoration.

Description

technical field [0001] The invention belongs to the field of cell technology, and in particular relates to a low-energy laser-induced vascularization kit for mesenchymal stem cells. Background technique [0002] As the largest organ of the human body, the skin has multiple functions such as sensation, temperature regulation, secretion and excretion, and prevention of water evaporation. It acts as a barrier between the human body and the external environment to maintain the stability of the internal environment, and is also an important part of the immune system. Extensive skin defects caused by trauma often result in very severe physical disability and even death. Angiogenesis is an important step in the treatment of skin defects, and is crucial to the recovery of skin wound-related functions. A variety of cells can be induced to differentiate in vitro to produce vascular endothelial cells. The vascular endothelial cells induced and differentiated from mesenchymal stem cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12N5/071C12N5/0775C12N13/00
CPCC12M35/02C12N5/069C12N13/00C12N2529/10C12N2506/1346C12N2500/35C12N2500/34C12N2500/32C12N2500/30
Inventor 汤苏阳
Owner 华夏(青岛)生物科技有限公司
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