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Preparation method and application of microbial agent for slope protection

A technology of microbial inoculants and mixed inoculants, applied in the field of preparation of microbial inoculants, can solve the problems of increasing the difficulty of slope protection, reducing the survival rate, soil pollution, etc., and achieving improvement and soil enzyme activity, promoting the formation, and improving the survival rate. Effect

Active Publication Date: 2020-12-18
北京航天恒丰科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, after the slope soil degrades, it becomes sandy and the water storage capacity is weakened; while the roots of slope protection plants grow slowly at the initial stage of planting, the degraded soil is not conducive to plant seed implantation and plant rooting and germination, which leads to a decrease in survival rate. Increased the difficulty of slope protection and weakened the ecological effect of slope protection
[0004] If a large amount of compound fertiliz

Method used

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  • Preparation method and application of microbial agent for slope protection
  • Preparation method and application of microbial agent for slope protection
  • Preparation method and application of microbial agent for slope protection

Examples

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preparation example 1

[0036] PDA medium: 21.0g sucrose, 20.0g agar, 200g potato; 1000ml distilled water, PH7.0-7.5;

[0037] PDB medium: (28°C, 180r / min, 72h): 20.0g sucrose, 200g potato; 1000ml distilled water, PH7.0-7.5;

[0038] Nutrient agar medium (NA): beef extract 5.0g, peptone 10.0g, sodium chloride 5.0g, agar 20.0g, distilled water 1000ml, PH7.0-7.2;

[0039] NB medium: peptone 10.0g, beef extract 5.0g, sodium chloride 5.0g, agar 20.0g, distilled water 1000ml, PH7.0-7.5;

[0040] BPY medium: beef extract 5.0g, sodium chloride 5.0g, glucose 10.0g, peptone 10.0g, yeast powder 5.0g, distilled water 1000ml, PH7.0-7.5;

[0041] All culture media were sterilized at 121°C for 30 minutes and set aside.

[0042]The Bacillus amyloliquefaciens FZB42 strain and Trichoderma harzianum stored at 4°C were activated with NA and PDA plates respectively, and cultured in a constant temperature incubator at 28°C for 2 days and 5 days respectively; Bacillus amyloliquefaciens was inoculated into BYP liquid med...

preparation example 2

[0052] The Bacillus amyloliquefaciens FZB42 strain and Trichoderma harzianum stored at 4°C were activated with NA and PDA plates respectively, and cultured in a constant temperature incubator at 28°C for 2 days and 5 days respectively; Bacillus amyloliquefaciens was inoculated into BYP liquid medium , 28°C, 180 rev / min constant temperature shaking incubator for 2 days; add 10ml sterilized physiological saline to each plate of the spore-filled Trichoderma harzianum strain, hang the sporangia with a spreader and inoculate it into the PDB medium In the 28°C, 180 rpm constant temperature shaking incubator, cultured for 5 days.

[0053] Among them, the amount of Bacillus amyloliquefaciens was 1 10 8 cfu / ml, the spore concentration of Trichoderma harzianum bacterial liquid was adjusted to 1 10 6 a / ml;

[0054] Weigh 51% rice straw, 25.5% wheat bran, 1.5% rice bran, 21.5% shell powder and 0.5% (NH 4 ) 2 SO 4 Mix to form a fermented material, add water to make the moisture c...

preparation example 3

[0059] The Bacillus amyloliquefaciens FZB42 strain and Trichoderma harzianum stored at 4°C were activated with NA and PDA plates respectively, and cultured in a constant temperature incubator at 28°C for 2 days and 5 days respectively; Bacillus amyloliquefaciens was inoculated into BYP liquid medium , 28°C, 180 rev / min constant temperature shaking incubator for 2 days; add 10ml sterilized physiological saline to each plate of the spore-filled Trichoderma harzianum strain, hang the sporangia with a spreader and inoculate it into the PDB medium In the 28°C, 180 rpm constant temperature shaking incubator, cultured for 5 days.

[0060] Among them, the amount of Bacillus amyloliquefaciens was 1 10 8 cfu / ml, the spore concentration of Trichoderma harzianum bacterial liquid was adjusted to 1 10 6 a / ml;

[0061] Weigh 51% rice straw, 25.5% wheat bran, 1.5% rice bran, 21.5% shell powder and 0.5% (NH 4 ) 2 SO 4 Mix to form a fermented material, add water to make the moisture c...

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Abstract

The invention provides a preparation method and application of a microbial agent for slope protection, and relates to the technical field of ecological restoration. The microbial agent is formed by mixed-fermentation-cultured bacillus amyloliquefaciens and trichoderma harzianum strains as well as arbuscular mycorrhizal fungi. The activity of rhizospheric microorganisms and soil enzymes can be improved, the number and activity of harmful microorganisms in soil are inhibited, and the survival rate of slope protection plants is increased; and in cooperative application with a straw board matrix,cellulose can be degraded quickly, and meanwhile, the formation of a soil aggregate structure is promoted, so that the survival rate of the slope protection plants is increased.

Description

technical field [0001] The invention relates to the technical field of ecological restoration, in particular to a preparation method and application of a microbial agent for slope protection. Background technique [0002] China's ecological environment is increasingly fragile. According to statistics, due to wind and sand activities, secondary salinization, and pasture overload and overgrazing, the soil quality of pastures is loose and landslides occur frequently, which not only increases the sediment concentration of rivers, And because livestock manure directly enters the river, it not only affects the environmental development of the pasture, but also threatens the ecological balance of the water environment around the pasture. Vegetation slope protection is the preferred ecological slope protection technology. [0003] However, after the slope soil degrades, it becomes sandy and the water storage capacity is weakened; while the roots of slope protection plants grow slow...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01B79/02A01G24/20A01G24/10A01G22/00A01G22/20A01G22/40E02D17/20C12R1/07C12R1/885C12R1/645
CPCA01B79/02A01G22/00A01G22/20A01G22/40A01G24/10A01G24/20C12N1/14C12N1/20E02D17/20
Inventor 刘海明李硕姜秀娟吕中文程淑琴杜蓉蓉
Owner 北京航天恒丰科技股份有限公司
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