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A kind of symbiotic fungus m2-1 of Pleurotus chinensis and its bacterial agent and fermentation liquid extract

A technology of symbiotic fungi and Psoriasis, which is applied in the field of microorganisms, can solve the problem of lack of antibacterial activity of symbiotic fungi and achieve broad-spectrum antibacterial activity, threat mitigation, and good inhibitory effect

Inactive Publication Date: 2022-04-22
YUNNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of a symbiotic fungus M2-1 with strong antibacterial activity and its application

Method used

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  • A kind of symbiotic fungus m2-1 of Pleurotus chinensis and its bacterial agent and fermentation liquid extract
  • A kind of symbiotic fungus m2-1 of Pleurotus chinensis and its bacterial agent and fermentation liquid extract
  • A kind of symbiotic fungus m2-1 of Pleurotus chinensis and its bacterial agent and fermentation liquid extract

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Experimental program
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Effect test

Embodiment 1

[0035] like Figure 1 to Figure 8 As shown, the present invention screens and obtains a kind of symbiotic fungus M2-1 (Mucorfragilis brittle mold) of P. of. The fermentation liquid extract of its strain culture is pathogenic to Bacillus lentus, Vibrio parahaemolyticus, Staphylococcus hemolyticus, Pseudomonas aeruginosa, Shigella flexneri, Bacillus subtilis, Escherichia coli, etc. Bacteria have good antibacterial activity. In order to alleviate the threat of pathogenic bacteria drug resistance to human health, research and development of new antibacterial drugs provide a new way.

[0036] Isolation and identification of the symbiont M2-1 strain of Pleurotus chinensis

[0037] 1. Isolation of Symbiotic Bacteria of Pseudomonas

[0038] Wash the fruiting body of Pleurotus chinensis with tap water earlier for 36 hours, then transfer to the aseptic workbench, and then rinse the fruiting body with 3L sterile water.

[0039] Use a sterilized knife to take 2g of Pleurotus sporocar...

Embodiment 2

[0051] Extraction of Active Substances from Symbiotic Bacteria M2-1

[0052] 1. Liquid fermentation culture

[0053] Liquid fermentation medium LMM: lactose 15g / L, sodium nitrate 6g / L, potassium chloride 0.52g / L, magnesium sulfate heptahydrate 0.52g / L, potassium dihydrogen phosphate 1.52g / L, trace elements 1ml / L, pure Water 1L. Media aliquots.

[0054] Add 700lul of MY liquid to a 2ml centrifuge tube, then scrape an appropriate amount of mycelium of Pleurotus chinensis M2-1 into the centrifuge tube, crush it for 90 seconds, and then inoculate the crushed solution into each tube Place in a Erlenmeyer flask with LMM liquid medium in a constant temperature shaking incubator at 150r min-1, and culture at 28°C for 10 days

[0055] 2. Extraction of cultured mycelia

[0056] After the fermentation culture is completed, the obtained fungal mycelium and culture solution. Use a separatory funnel to separate the mycelium and the bacterial liquid, add ethyl acetate (1:1) to the bacte...

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Abstract

The invention discloses a symbiotic fungus M2‑1 of Pleurotus chinensis and a fermentation broth extract of its strain culture. A symbiotic fungus M2-1 of the present invention, the bacterial strain is the symbiotic fungus M2-1 (Mucor fragilis); the preservation name is Mucor fragilis YAFM2-1; it is preserved in the Chinese Type Culture Collection Center, The deposit address is Wuhan University, Wuhan, China; deposit date: July 16, 2020; deposit number: CCTCC NO: M 2020322 The present invention has a good inhibitory effect on a variety of pathogenic bacteria. Threats posed to human health, research provides new avenues for the development of new antibacterial drugs.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a symbiotic fungus M2-1 of Pleurotus chinensis and its bacterial agent and fermentation liquid extract. Background technique [0002] Pathogenic bacteria can make people, animals and plants sick, and bring us huge health, food and property losses every year. In order to kill germs or inhibit the activity of germs, people have invented antibiotics and synthetic antibacterial drugs. With the widespread use of antibacterial drugs application, pathogenic bacteria are resistant to antibiotics, and even multi-drug resistance has emerged. The occurrence and spread of pathogenic bacteria resistance has constituted a serious threat to human health, so looking for emerging biological resources is a promising path. At present, there are more and more studies on the secondary metabolites of symbiotic bacteria. During the long-term co-evolution process, symbiotic bacteria and host pla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/06A61P31/04C12R1/785
CPCC12N1/14A61K36/06A61P31/04
Inventor 王毅罗婷
Owner YUNNAN ACAD OF FORESTRY
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