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Peptide KC-19 with antibacterial activity

A technology of KC-19 and antibacterial activity, applied in the field of peptide KC-19, can solve problems such as polluting the environment, limited range of action of pesticides, and endangering human health

Inactive Publication Date: 2021-10-22
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of pesticides not only pollutes the environment but also endangers human health, and the scope of pesticide action is limited

Method used

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  • Peptide KC-19 with antibacterial activity
  • Peptide KC-19 with antibacterial activity
  • Peptide KC-19 with antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1 Materials and methods

[0024] 1.1 Experimental materials

[0025] 1.1.1 Pathogenic bacteria

[0026] Olivia umbilicali, Curvularia lunata and Fusarium graminearum are preserved in this laboratory.

[0027] 1.1.2 Medium

[0028] The medium used in this study was potato dextrose agar (PDA), configured as follows:

[0029] (1) 200g of fresh potatoes, peeled, cut into pieces and put into 1000ml of distilled water.

[0030] (2) After boiling in water for 20 minutes, filter with gauze.

[0031] (3) Add 20 g of sucrose and 20 g of agar powder to the filtrate, and add distilled water to make the volume to 1000 ml.

[0032] (4) After autoclaving at 121°C for 21 minutes, pour it onto a plate for later use.

[0033] 1.2 Experimental method

[0034] 1.2.1 Synthesis of peptides

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Abstract

The invention discloses a peptide KC-19 with antibacterial activity. The amino acid sequence of the peptide KC-19 is KCHLHPLCKGEALELFSVG. A broad-spectrum antifungal activity experiment of the peptide KC-19 shows that the peptide KC-19 achieves an inhibition effect on fungi mainly through hypha aggregation. The highest inhibition rates of the KC-19 on Bipolaris zeicola, Curvularia lunata, Fusarium graminearum are 47.0%, 47.8% and 25.1% respectively.

Description

technical field [0001] The present invention relates to a peptide KC-19 with antibacterial activity. Background technique [0002] Fungal plant pathogens are an important class of microorganisms that cause significant economic losses to agricultural production around the world. For decades, chemical pesticides have been commonly used to control the growth and spread of fungal pathogens. However, the use of pesticides not only pollutes the environment but also endangers human health, and the range of effects of pesticides is limited. Therefore, there is an urgent need to find a green, environmentally friendly and safe new fungicide to replace the currently used chemical pesticides. [0003] Antimicrobial peptides are a class of polypeptides that widely exist in the entire biological world. Antimicrobial peptides can act as part of the immune system of plants, insects or mammals to resist the invasion of pathogenic microorganisms. Antimicrobial peptides have the characteri...

Claims

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Application Information

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IPC IPC(8): C07K7/08A01N43/50A01P3/00
CPCC07K7/08A01N43/50
Inventor 吴刘记陈雪艳贾兴锰
Owner HENAN AGRICULTURAL UNIVERSITY
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