36 results about "Curvularia lunata" patented technology

The invention relates to a method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs, and relates to a preparation method of the fermentation liquor. The method mainly solves the technical problems that chemical pesticides are unsafe, and microorganism sourced pesticide is few in kind. The method comprises the steps that (1) excitation cultivation of strains is carried out, (Streptomyces sp.) Syjd3 is shifted to gause first solid media for cultivation to obtain activated (Streptomyces sp.) Syjd3; (2) the activated (Streptomyces sp.) Syjd3 is inoculated to the gause first solid media, the gause first solid media after the inoculation are put into a shake cultivation box for cultivation, and the streptomyces fermentation liquor for preventing and treating the alamo grey speck disease germs is obtained. The streptomyces fermentation liquor has an inhibition function on the alamo grey speck disease germs, and also has certain inhibition functions on alamo leaf blight germs, fusarium solani, wheat root rot germs, curvularia lunata and rice bakanae pathogen.

The invention discloses streptomyces with an inhibiting effect on poplar gray leaf spot pathogen, relating to a strain of streptomyces. The invention provides the streptomyces which has the inhibiting effect on the poplar gray leaf spot pathogen and provides an effective way for biological prevention and control of poplar gray leaf spot. The streptomyces with the inhibiting effect on the poplar gray leaf spot pathogen is streptomyces Syjd3, and is preserved in the Common microorganism Center of China Committee for Culture Collection of Microorganisms; the preservation address of streptomyces is No. 3, No.1 Institute, west Beichen Road, Chaoyang District, Beijing; the preservation date of streptomyces is June 17, 2014; the preservation number of streptomyces is CGMCC No.9355. Streptomyces Syjd3 disclosed by the invention has the inhibiting effect on the poplar gray leaf spot pathogen, and also has a certain inhibiting effect on poplar stemphylium botryosum, fusarium solani, bipolaris sorokiniana, curvularia lunata and fusarium moniforme.

The invention provides a method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium, relating to a method for preparing a compound II by adopting 4-alkene-3,20-dione steroid I as an initiator through microbial co-fermentation. The method is characterized in that the 11beta-hydroxyl-1,4-diene-3,20-dione steroid II is prepared through the mixed one-step fermentation of curvularia lunata AS 3.4381 and arthrobacter simplex AS1.94.

The invention discloses an application of an indolyl diketopiperazine compound in preparation of biological pesticides. According to the application, the discovered compound, i.e., 1,2,3,4-tetrahydro-2-methyl-3-methylene-1,4-dioxopyrazino[1,2-a]indole has a very high inhibiting effect on phytopathogens, i.e., Curvularia lunata, Colletotrichum gloeosporioides, Alternaria alternata and Cylindrocladium scoparium, so that the compound, i.e., 1,2,3,4-tetrahydro-2-methyl-3-methylene-1,4-dioxopyrazino[1,2-a]indole has a very good phytopathogen inhibiting effect and has a good application development prospect. Therefore, the invention provides candidate biopesticides for researching and developing novel antiphytopathogen pesticides and provides a scientific basis for developing and utilizing marine fungus derived natural active substances.

The invention provides a broad-spectrum functional bacteria (Bacillus simplex)CGMCC No.16458, which has high salt tolerance, multi-function repair and continuous cropping, and salt and alkali resistance for soil resistance. The strain has high ability to dissolve phosphorus, fix nitrogen, produce ferritin, ACCD and cytokinin, and has a salt tolerance of 10%, and has strong antagonistic effects forPythium, Fusarium, Curvularia lunata, Bipolaria maydis and Phytophthora, is suitable for the production of the biological fertilizer with disease prevention, growth promotion, salt and alkali resistance, and the like, and is applied to medium and heavy saline-alkali land production and forage cultivation, as well as from production of vegetables for many years of continuous cropping facilities, the strain promotes emergence, promotes root development, prevents root diseases, improves crop yield and quality, can reduce the use of chemical fertilizers and pesticides, and has broad application prospects.

The invention provides a curvularia lunata steroid C11 beta-hydroxylase mutant L6 and a curvularia lunata steroid C11 beta hydroxylase mutant L7. The DNA sequences are shown as DNA SEQ ID NO: 14 and DNA SEQ ID NO: 16. Compared with C11 beta-hydroxylase CYP5103B6, the specificity of converting steroid substrates 11-deoxycortisol by the mutants L6 and L7 is obviously improved, and byproducts are obviously reduced. The invention further provides an expression vector based on heterologous overexpression of mutants L6 and L7 and a steroid transformation process of the expression vector, and precious materials (genes and strains) and basic data support are provided for researching and developing a process for producing hydrocortisone by efficient C11 beta-hydroxylation 11-deoxycortisol.

The invention belongs to the technical field of biotechnology and steroid drug conversion, and particularly relates to the application of Curvularia crescenae CICC40301 in C14α-hydroxylation. Curvularia lunata (Curvularia lunata) is used for C11β hydroxylation in industry, and the present invention discovers Curvularia lunata with C14α hydroxylation ability for the first time, and Curvularia lunata CICC40301 can transform 4AD into C14α-4AD, yield Reaching 40%‑50%, this discovery will provide a new route for the C14α hydroxylation of steroids.

The invention discloses a bacteria medicament mixture for preventing and controlling weed in a rice field, which is characterized in that: curvularia lunata strain B6 (Wakker) Boedijn with the collection number of CGMGGNO.2928 and cyhalofop-butyl are adopted as active ingredients and proper solvent, surfactant, thickener and stabilizer are adopted as auxiliary materials, and then the active ingredients and the auxiliary materials are subjected to mechanical grinding to prepare a suspension system, wherein the concentration of the active ingredient curvularia lunata strain B6 is (0.1-10)*10<8>spores / ml, and the mixture comprises the following components in percentage by mass: 0.1 to 10 percent of cyhalofop-butyl, 1 to 10 percent of surfactant, 1 to 10 percent of stabilizer, 0.5 to 5 percent of thickener and the balance of solvent. The bacteria medicament mixture is used for preventing and controlling various kinds of weed in the rice field and has the advantages of safety for rice, wide weed controlling range and capability of controlling the weed in the entire growth period of the rice. The invention provides a new herbicide prepared by compounding stable and efficient post-emergence microorganisms with a chemical pesticide, and the new bacteria medicament mixture is environmental-friendly, prevents and controls various kinds of weed in the rice field and obviously increases the yield.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and / or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention discloses a deep-sea fungus Cladosporium and its extract and application. The deep-sea fungus Cladosporium sp. FS86 was preserved in the China Center for Type Culture Collection (CCTCC) on March 27, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2014108. Its fermented extract To pathogenic fungi Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletotrichum gloeosporioides, Cylindrocladium scoparium ) or Curvularia lunata had significant inhibitory effect. Therefore, the realization of the present invention provides a scientific basis for the development of new anti-pathogenic fungal drugs and the exploration of natural active substances derived from deep-sea fungi.

The invention provides a microbial flocculant composition and a preparation method thereof and belongs to the field of wastewater treatment. In order to overcome the shortcomings of an inorganic flocculant and an organic flocculant in the prior art, the biological composite flocculant of the invention comprises the following raw materials, by weight, of 30-40 parts of co-fermentation products of curvularia lunata and rhodococcus erythropolis, 50-95 parts of activated silicate and 3-6 parts of polyoxyethylene. The microbial flocculant composition has the advantages of being high in flocculation activity, safe, non-toxic, free of secondary pollution, and suitable for being applied and popularized.

The invention provides a curvularia lunata strain and application thereof. The strain is curvularia lunata B6 of which the preservation number is CGMCC No.2928. The curvularia lunata strain B6 CGMCC No.2928 has strong and fast pathogenicity to echinochloa crusgalli; field experiments show that the echinochloa crusgalli shows spot and dead symptoms in a week after spores of the curvularia lunata strain are sprinkled to the echinochloa crusgalli and the fresh weight inhibition rate to the echinochloa crusgalli can reach 90 percent in 28 days. The fungus strain curvularia lunata strain B6 CGMCC No.2928 is separated from echinochloa crusgallis naturally having diseases in fields without any artificial gene modification; therefore, the curvularia lunata strain cannot have any ecological and environmental hidden danger when released to nature and is an absolutely-safe biological prevention and control medium.

The invention belongs to the field of biotechnology, and in particular relates to an SSR primer for analyzing the genetic diversity and kinship of Curvularia lunene and its application. The nucleotide sequences of the SSR primers used for the analysis of the genetic diversity and kinship of Curvularia lunata are shown in SEQ ID NO.1-40. The present invention also provides a kit for analyzing the genetic diversity and kinship of Curvularia lunata, and the development of the primers and the kit provides a new technique for studying the genetic diversity and kinship analysis of Curvularia lunene ways and means.

The present invention relates to a plant mangrove-associated fungus Culvularia lunata, and a simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove-associated fungus Curvularia lunata, said method comprising steps of cutting the leaves into small pieces and placing it on potato dextrose agar (PDA) plates for 48 hours, maintaining the culture at temperature ranging between 26-47° C. and salinity ranging between 4-32 ppt with occasional stress conditions for about 17-19 days to obtain mycelial mat, sonicating the mat to lyse the cells, extracting crude from the sonicated mat using methanol repeatedly, concentrating the crude, treating the concentrated crude extract with solvents of increasing polarity, obtaining aqueous fraction as white powder residue after the treatment containing crude mannitol, purifying the crude mannitol by chromatography to obtain pure mannitol with about 75% of the total crude extract.