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Early identification method for curvularia leaf spot in maize inbred lines

The invention relates to a technology for the leaf spot disease and identification method of Curlyspora, which is applied in the field of disease resistance breeding, and can solve the problems of being susceptible to other diseases and regions, difficult to identify and screen, complicated steps, and the like, so as to reduce the complicated work, the method is accurate, the The effect of breaking seasonal restrictions

Inactive Publication Date: 2012-10-24
QINGDAO AGRI UNIV
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AI Technical Summary

Problems solved by technology

In the breeding of resistance to Curvularia spp. leaf spot disease in corn, the identification of its disease resistance mainly adopts the method of artificially inoculating Curvularia spore suspension, and then using the disease index such as the number of diseased spots and the amount of sporulation to evaluate the resistance ( Wang Hong et al. Journal of Shenyang Agricultural University, 2000, 31 (5): 476-478; Zhao Laishun et al. Journal of Hebei Agricultural University, 1995, 18 (4): 36-38), this identification method has a large workload, and Susceptible to other diseases and regions (Diao Yi et al. Southern Agricultural Journal, 2011, 42(2): 161-163; Leng Tingrui et al. Modern Agricultural Science and Technology, 2010, (17): 159-161, 163); Other identification methods for disease resistance are isoenzyme analysis, enzyme-linked immunoassay, etc. Although these methods are accurate, the steps are cumbersome, the cost is high, and it is difficult to carry out large-scale identification and screening. Therefore, it is urgent to find a simple and reliable method. , and can identify the resistance of corn inbred lines Curvularia leaf spot indoors

Method used

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  • Early identification method for curvularia leaf spot in maize inbred lines
  • Early identification method for curvularia leaf spot in maize inbred lines

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Preparation of Curvularia lunata toxin solution

[0032] 1. Cultivate the Curvularia lunata strain (this bacterial strain is isolated and identified by Mr. Yan Honghai, Plant Pathology Teaching and Research Office, Qingdao Agricultural University) on PDA medium (its composition and weight ratio are: every 1L of distilled water contains 200g of potatoes and glucose 15g), after cultivating for 7 days, use a puncher (Φ=0.7cm) to punch holes on the cultured bacterium colony, and connect 6 Curvularia lunata bacteria sheets in 100mL improved Fries liquid medium (its composition and weight The ratio is: every 1L of distilled water contains 20g of sucrose, 5g of ammonium tartrate, and NH 4 NO 3 1g, KH 2 PO 4 1g, 0.1g of sodium chloride, 0.5g of magnesium sulfate heptahydrate, 0.13g of calcium chloride and 1g of yeast extract, pH 6.2), and cultured at 25°C and 120rpm for 15d; Granular activated carbon (30g) filter the above liquid medium at a flow rate of 4.2mL / m...

Embodiment 2

[0033] Example 2 Comparative Test of Early Identification of Curvularia Leaf Spot Resistance in Maize Inbred Lines

[0034] Proceed as follows:

[0035] Test materials: 10 maize inbred lines: Qi 319, Q58, Chang 7-2, Zheng 58, Mei 26800, Dan 340, Zheng 0510, Shan 814, 189, Danhuang 25.

[0036] Test design:

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Abstract

The invention belongs to the field of crop breeding for disease resistance and relates to an early identification method for curvularia leaf spot in maize inbred lines. The early identification method for curvularia leaf spot in maize inbred lines includes treating leaves of three-leaf seedlings of maize inbred lines in maize curvularia lunata toxin solution, and mixing with sodium borate buffer solution to extract enzyme crude extract of phenylalnine ammonialyase, peroxidase and superoxide dismutase in the leaves; measuring enzyme activities of the phenylalnine ammonialyase, the peroxidase and the superoxide dismutase, and calculating specific activity; calculating resistance values according to a formula, and calculating average of the resistance values. The average larger than 3 is the value of resistance of the inbred line to maize curvularia leaf spot. The method is accurate and reliable. The method can be used to identify and screen indoors without limitations of external environment conditions. In addition, the method has no season limitation in seedling-stage disease resistance identification, and shortens identification time. The method is high in work efficiency.

Description

technical field [0001] The invention belongs to the field of disease-resistant breeding, and in particular relates to an early identification method for the resistance of corn inbred lines Curvularia leaf spot. Background technique [0002] Corn Curvularia Leaf Spot Disease (Corn Curvularia Leaf Spot Disease) is a new disease with great harm that occurred in North China in the middle and late 1980s. It mainly damages leaves, and also damages leaf sheaths and bracts. After tasseling, the disease rapidly expands and spreads, the plants are covered with scabs, and the leaves dry up early, generally reducing production by 20-30%, and severe plots reduce production by more than 50%, or even fail. [0003] The pathogenic bacteria of Curvularia lunata (Wakker) Boed and Curvularia inaeguacis belong to the genus Curvularia lunata. The prevention and control of Curvularia maize leaf spot mainly involves disease-resistant breeding, cultivation, and chemical agents, among which cultiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/527C12Q1/28C12Q1/26
Inventor 郭新梅宋希云叶克苁裴玉贺张恩盈
Owner QINGDAO AGRI UNIV
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