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Functional small peptide KP-11 and application thereof

A KP-11, functional technology, applied in applications, peptides, chemicals for biological control, etc., can solve problems such as polluting the environment, limited range of action of pesticides, and endangering human health

Inactive Publication Date: 2021-11-05
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of pesticides not only pollutes the environment but also endangers human health, and the scope of pesticide action is limited

Method used

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  • Functional small peptide KP-11 and application thereof
  • Functional small peptide KP-11 and application thereof
  • Functional small peptide KP-11 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] 1 Materials and methods

[0025] 1.1 Experimental materials

[0026] 1.1.1 Pathogenic bacteria

[0027] The Helmitosporium umbilifera, Curvus crescentus and Helminthium maize were preserved by our laboratory.

[0028] 1.1.2 Culture medium

[0029] The medium used in this study is potato dextrose agar (PDA), and the configuration is as follows:

[0030] (1) 200g of fresh potatoes, peeled and cut into pieces and put into 1000ml of distilled water.

[0031] (2) After boiling for 20 minutes, filter with gauze.

[0032] (3) 20g sucrose and 20g agar powder were added to the filtrate, and distilled water was added to make the volume to 1000ml.

[0033] (4) After autoclaving at 121°C for 21 min, pour the plate into a plate for later use.

[0034] 1.2 Experimental method

[0035] 1.2.1 Peptide synthesis

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Abstract

The invention discloses a functional small peptide KP-11. The amino acid sequence of the peptide KP-11 is KPWLRVALCPG. A broad-spectrum antifungal activity experiment of the peptide KP-11 shows that the peptide KP-11 achieves an inhibition effect on fungi mainly through hypha aggregation. The highest inhibition rates of the peptide KP-11 on corn bihilum, curvularia lunata and maize bihilum are respectively 76.9%, 62.3% and 45.7%.

Description

technical field [0001] The invention relates to a functional small peptide and its application, in particular to a functional small peptide with antibacterial activity against pathogenic fungi. Background technique [0002] Fungal phytopathogens are an important class of microorganisms that cause significant economic losses to agricultural production around the world. For decades, chemical pesticides have been commonly used to control the growth and spread of fungal pathogens. However, the use of pesticides not only pollutes the environment but also harms human health, and the scope of pesticides is limited. Therefore, there is an urgent need to find a green, environmentally friendly and safe new fungicide to replace the chemical pesticides currently in use. [0003] Active peptides are called functional small peptides, and their molecular structures vary in complexity, ranging from simple dipeptides to cyclic macromolecular polypeptides, and these peptides can be modified...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A01N47/44A01N37/46A01P3/00
CPCC07K7/06A01N47/44A01N37/46
Inventor 吴刘记陈雪艳贾兴锰王晓旭
Owner HENAN AGRICULTURAL UNIVERSITY
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