Method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium

A combined fermentation and compound technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve problems such as difficult and large enzyme activity

Active Publication Date: 2016-07-20
TIANJIN JINYAO GRP
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the joint transformation fermentation is technically very difficult, mainly due to the different growth and fermentation conditions of different bacteria, such as: different temperature, medium, and pH value, it is difficult to make the two bacteria exert their maximum enzyme activity under the same fermentation conditions

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium
  • Method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium
  • Method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The selection of embodiment 1 fermentation medium

[0055] In the following examples, the influence of the fermentation medium on the conversion rate of the target product was examined. Incline, seed cultivation method as described in the summary of the invention.

Embodiment 1-1

[0057] In 1L of water, 5g of fermentation substrate RS, 30g of glucose, 5g of beef extract, 15g of corn steep liquor, 2g of ammonium sulfate, 1g of magnesium sulfate, 5g of sodium chloride, 0.4g of foam enemy, and 0.4g of foam enemy in 1L of water, the pH is 5.5. For the secondary culture of Arthrobacter simplex, after 24 hours of primary seed culture, insert 10% of the inoculum into the secondary seed medium, and after 22 hours of secondary culture, insert 15% of the inoculum into the above fermentation medium ; After 24 hours of culturing the primary seeds of Curvularia crescens, they were inserted into the above-mentioned fermentation medium according to 30% inoculum amount. Use a 30L fermenter for conversion, with a liquid volume of 80%, a tank pressure of 0.05MPa, and an air flow of 40m 3 / hr, temperature 28°C, stirring 180rpm, conversion for 48 hours, sampling by TLC analysis, the conversion of the substrate was complete, the sample was extracted with ethyl acetate, the ...

Embodiment 1-2

[0060] The components of the fermentation medium are 10g of glucose, 5g of beef extract, 15g of corn steep liquor, 2g of ammonium sulfate, 1g of magnesium sulfate, 5g of sodium chloride, 0.4g of foam enemy, and the pH is 5.5. Other conditions are the same as in Example 1-1.

[0061] After TLC analysis, the conversion of the substrate was complete, the sample was extracted with ethyl acetate, and the upper layer was sent to HPLC, the prednisolone content was 77.1%.

[0062] After the conversion is complete, the fermentation broth is inactivated at 85°C, cooled to room temperature, and filtered with suction. The filter cake was extracted with ethyl acetate and concentrated to obtain white crystals with a crude product yield of 89.2% and a prednisolone content of 78.4%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for preparing 11beta-hydroxyl-1,4-diene-3,20-dione steroid through co-fermentation of curvularia lunata and arthrobacterium, relating to a method for preparing a compound II by adopting 4-alkene-3,20-dione steroid I as an initiator through microbial co-fermentation. The method is characterized in that the 11beta-hydroxyl-1,4-diene-3,20-dione steroid II is prepared through the mixed one-step fermentation of curvularia lunata AS 3.4381 and arthrobacter simplex AS1.94.

Description

technical field [0001] The invention relates to a production method of steroid medicines, in particular to one-step preparation of 11β-hydroxyl-1,4-diene-3,20-dione steroids by combined fermentation of Curvularia lunae and Arthrobacter. Background technique [0002] Steroid drugs are an important class of drugs, with anti-inflammatory, anti-immune, contraceptive, anti-cancer, endocrine regulation and other functions, widely used in rheumatism, cardiovascular disease, collagen disease, lymphatic leukemia, human organ transplantation, Treatment of bacterial encephalitis, skin diseases, endocrine disorders, senile diseases, hormone-dependent tumors and other diseases. [0003] In the production of steroid hormone drugs, multiple groups of the steroid nucleus need to be modified by chemical methods and microorganisms, among which C 11 β hydroxylation and C 1,2 The introduction of double bonds by dehydrogenation is an important steroid transformation reaction, which is of great...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P33/02C12R1/645C12R1/06
Inventor 赵春霞王淑丽韩昆颖
Owner TIANJIN JINYAO GRP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap