Zopfiella sp. and application thereof

A technology of sporosporium and stipe, which is applied in the field of endophytic podosporium in Boju, which can solve the problems of insecticide resistance, ecological balance damage, and non-target organism damage.

Active Publication Date: 2018-06-19
FUYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main means of prevention and control in various countries today is chemical agents, which will inevitably bring a series of serious problems such as

Method used

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  • Zopfiella sp. and application thereof
  • Zopfiella sp. and application thereof
  • Zopfiella sp. and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the screening of endophytic Podosporum BJF10 of Boju chrysanthemum

[0033] The test pathogens used in this embodiment: Fusarium moniliforme, Fusarium oxysporum, Curvularia lunata, and Pythium. The pathogenic bacteria to be tested were respectively activated on PDA medium at 28°C for 4-5 days for later use.

[0034] The screening steps of the present embodiment mainly include:

[0035] (1) Acquisition of endophytic fungi of Boju: Rinse the healthy plants of Boju with running water and dry them, cut them into several parts and put them in the ultra-clean workbench, soak them in 75% alcohol for 3 minutes, and then wash them with sterile water Rinse 3 times, then rinse with 0.1% mercury liter for 30 sec, and then rinse with sterile water 3 to 4 times. The PDA and LB mediums were used for culture respectively, and the bacterial culture temperature was 37°C, and the fungal culture temperature was 28°C. After culturing for 3-4 days, pick the colonies accord...

Embodiment 2

[0042] Embodiment 2, Morphological and molecular identification of endophytic Podosporum BJF10 of Boju chrysanthemum

[0043] (1) Morphological identification of endophyte BJF10

[0044] Macroscopic traits such as colony size, color, decoration on the surface of the colony, and edge of the colony were observed by plate culture method. The microscopic properties such as hyphae and spore morphology of the active fungal cultured on inserts were observed under the low-power lens and 40-fold objective lens by microscopy.

[0045] After culturing and observing, the specific colony morphology of the endophytic fungus BJF10 is shown in Table 2 and figure 2 , fungal hyphae and spore morphology see image 3 .

[0046]

[0047] (2) Molecular biology identification of endophyte BJF10

[0048] Reagents involved in the embodiments of the present invention:

[0049] (1) DNA extraction reagents

[0050] TE solution: 10mmol / L Tris·HCl (pH8.0), 1mmol / L EDTA;

[0051] 3M NH 4 AC: Wei...

Embodiment 3

[0069] Example 3, the test of the endophytic Podosporum BJF10 of Boju chrysanthemum on the degradation function of cellulose

[0070] The medium involved in this example: 1) CMC-Na medium (g / L): NaCl 6.0g, MgSO 4 ·7H 2 O 0.1 g, KH 2 PO 4 0.5g, CaCl 2 0.1g, K 2 HPO 4 2.0g, CMC-Na 10.0g, (NH 4 ) 2 SO 4 2.0 g, 15.0 g agar, pH value 7.0~7.4. 2) CMC enzyme activity detection liquid fermentation medium (g / L): peptone 10.0 g, beef extract 10.0 g, CMC-Na 10.0 g, NaCl 1.5 g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.3g, distilled water, pH 7.0. 3) Filter paper enzyme activity detection liquid fermentation medium (g / L): peptone 10.0g, beef extract 10.0g, Xinhua filter paper 0.05g, NaCl 1.5g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.3g, distilled water 1000mL, pH value 7.0g. All the above mediums were sterilized at 0.1MPa, 121°C for 25 min, and cooled to 40-50°C for later use.

[0071] Solutions related to identification of cellulose degradation: 1) 1mg / mL Congo red solution: wei...

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Abstract

The invention discloses a zopfiella sp. multifunctional biocontrol characteristic strain. A preservation number of zopfiella sp. is CGMCC No. 60123. The zopfiella sp. BJF10 has strong inhibition effect for fusarium moniliforme, fusarium oxysporum, curvularia lunata, and pythium, through determination of cellulose degrading capability, CMC enzyme activity can achieve as high as 180.32 U/mL, and filter paper enzyme activity can achieve as high as 81.46 U/mL. Through determination of antioxidation capability, and the strain fermentation product has an average removing rate on DPPH can achieve 67.7%.

Description

technical field [0001] The invention relates to the field of biological control, and relates to a strain of endophytic Podospora chrysanthemum and application thereof. Background technique [0002] Plant disease control is a primary problem in agricultural production. However, chemical agents are the main means of prevention and control in various countries today, which will inevitably bring residual pollution to the biosphere, easily cause bacteria and pests to develop drug resistance, harm non-target organisms, and even destroy ecological balance. A series of serious problems. Microbial control products overcome the shortcomings of traditional chemical pesticides, fertilizers, etc., and show significant antagonism to plant diseases. The growth and metabolism of some microorganisms have a good growth-promoting effect on plants, and the effect is efficient and does not pollute the environment. High safety performance , in line with the strategic policy of environment-friend...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 唐俊王贵生陆娟唐娟朱玉李庆芬
Owner FUYANG NORMAL UNIVERSITY
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