Burkholderia and application thereof

A technology of Burkholderia and Holderia, applied in the field of microorganisms, can solve the problems of increased burden on farmers, increased production costs, and decreased quality of pods, so as to improve the soil microbial environment, promote growth, and eliminate continuous cropping The effect of biological barriers

Active Publication Date: 2014-03-19
SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, vanilla orchid is a perennial crop. With the increase of planting years, the soil microbial flora becomes imbalanced, the number of soil-borne pathogens increases significantly, and the diversity of soil microorganisms tends to be simple. In order to reduce the number of pathogens, a large number of pesticides are used in production, resulting in production Increased costs, increased burden on farmers, decreased quality of pods, and serious pollution of the ecological environment

Method used

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  • Burkholderia and application thereof
  • Burkholderia and application thereof
  • Burkholderia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: the isolation and identification of Burkholderia described in the present invention

[0030] The rhizosphere soil with better growth in the 20-year-old vanilla orchid plantation was selected, and the samples were taken back to the laboratory for dilution, coating, and cultivation. Select the strains with obvious phosphate-dissolving circles on the NBRIP selective medium, and then re-screen the high-efficiency growth-promoting bacteria V-29 through pot experiments. The strain V-29 was identified by 16S rDNA method, which belonged to Burkholderia stabilis, could solubilize phosphorus, and grew well under the condition of starch and beef extract as the only carbon and nitrogen sources.

[0031] The Burkholderia bacterium was preserved on August 3, 2013 in the General Microbiology Center of the Chinese Microbiological Culture Collection Management Committee, the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, ...

Embodiment 2

[0032] Embodiment 2: the preparation of microbial fertilizer of the present invention

[0033] Inoculate Burkholderia bacterium with preservation number CGMCC No.7980 in NA liquid medium with a pH value of 6-8, shake and culture at 28°C-35°C, 160-220 rpm for 36 hours, Centrifuge the bacteria, resuspend with sterile water, centrifuge again, and suspend with an equal volume of sterile water to obtain a bacterial suspension; among them, the formula of the NA liquid medium is: beef extract 5g / L, peptone 10g / L, chloride Sodium 5g / L, the balance is water.

[0034] Inoculate the bacterial suspension into the decomposed cow dung (the volume mass ratio of the bacterial suspension to the decomposed cow dung is 2mL:10g) for solid fermentation until the number of Burkholderia bacteria reaches 10 8 cfu / g or more, during which the solid fermentation temperature is not higher than 50°C, turning once a day to obtain microbial fertilizer. Among them, the mass ratio of organic matter in decom...

Embodiment 3

[0037] Embodiment 3: pot experiment

[0038] Collect 20-year-old vanilla orchid plantation soil with obvious continuous cropping biological obstacles as the test soil. The test pot uses a turnover box (inner size 600×420×165mm), and selects healthy vanilla orchids with the same growth as the test. Seedling.

[0039] Inoculate the strain V-29 marked with green fluorescent protein molecules into 2000ml NA liquid medium containing 0.3mg / ml kanamycin, culture on a shaker for 36h, centrifuge the bacteria, resuspend with sterile water, and centrifuge again. The bacterial suspension obtained after suspending with an equal volume of sterile water; wherein, the formula of the NA liquid medium is: beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L, and the balance is water.

[0040] Inoculate the bacterial suspension into the decomposed cow dung (the volume mass ratio of the bacterial suspension to the decomposed cow dung is 2mL:10g) for solid fermentation until the number of Burkh...

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Abstract

The invention relates to the microbial field, and specifically discloses Burkholderia. The Burkholderia disclosed by the invention is preserved in the China General Microbiological Culture Collection Center with the preserving number of CGMCC No.7980. The Burkholderia disclosed by the invention can be stably colonized in soil, and the microbial fertilizer of the Burkholderia can improve the microbial environment of the soil, eliminate successive cropping biological obstacles and have a remarkable inhibiting effect on fusarium oxysporum. Furthermore, the Burkholderia can promote growth of vanilla, and can be applied to prevention and treatment of fusarium oxysporum and promoting growth of vanilla.

Description

technical field [0001] The present invention relates to the field of microbes, and more specifically relates to a Burkholderia and its application. Background technique [0002] Vanilla planifolia (Vanilla planifolia Andrews) is a tropical vine spice plant of Orchidaceae, and has the reputation of "the king of natural food spices". Its fresh bean pods contain more than 250 kinds of aromatic components after aroma processing, and are widely used in the preparation of various high-end cigarettes, famous wines, and tea leaves. [0003] Due to the high requirements of vanilla orchid on climatic conditions, the current vanilla orchid planting areas in my country are mainly distributed in Hainan Province, but Hainan Province has abundant precipitation, strong leaching effect, high soil acidity, iron, aluminum and phosphorus in the soil form insoluble iron and phosphorus and aluminum phosphorus, and even the less effective closed-storage phosphorus, so that most of the soil phospho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F3/00C05F3/02A01P3/00C12R1/01
CPCY02A40/20
Inventor 赵青云王华庄辉发赵秋芳王辉宋应辉朱自慧
Owner SPICE & BEVERAGE RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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