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Bacillus cereus and application of bacillus cereus

A technology of Bacillus cereus and Fusarium oxysporum, applied in the field of microorganisms, can solve the problems of increased burden on farmers, increased production costs, and decreased quality of pods, and achieve the effects of improving the soil microbial environment, promoting growth, and eliminating biological obstacles to continuous cropping

Active Publication Date: 2014-03-26
海南兴科热带作物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to reduce the number of pathogenic bacteria, a large number of pesticides are used in production, resulting in increased production costs, increased burden on farmers, decreased quality of pods, and serious pollution of the ecological environment

Method used

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  • Bacillus cereus and application of bacillus cereus
  • Bacillus cereus and application of bacillus cereus
  • Bacillus cereus and application of bacillus cereus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Isolation and identification of Bacillus cereus of the present invention

[0034] The rhizosphere soil with better growth in the 20-year-old vanilla orchid plantation was selected, and the samples were taken back to the laboratory for dilution, coating, and cultivation. Select the strains with obvious hemolytic circles on the blood agar medium (indicating that they can produce antifungal lipopeptide active substances), and then re-screen out the pathogenic bacteria of vanilla orchid root rot-Fusarium oxysporum through the plate confrontation test. Significant inhibitory effect of strain F-6. The strain F-6 was identified by 16S rDNA method. The strain F-6 belonged to Bacillus cereus, could produce antifungal lipopeptide substances, and grew well under the condition of maltose and peptone as the only carbon and nitrogen sources. The strains were stored at -80°C in 30% glycerol.

[0035]The Bacillus cereus has been preserved in the General Microbiology Cen...

Embodiment 2

[0036] Embodiment 2: the preparation of biocontrol agent of the present invention

[0037] Inoculate Bacillus cereus with a preservation number of CGMCC No.7979 in NA liquid medium with a pH value of 6-8, shake and culture at 28°C-35°C, 160-220 rpm for 36 hours, and centrifuge the bacteria , resuspend with sterile water, centrifuge again, suspend with equal volume of sterile water and add carrier oil, activator and UV protection agent.

[0038] Among them, the formula of NA liquid medium is: beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L, and the balance is water.

Embodiment 3

[0039] Embodiment 3: the preparation of microbial fertilizer of the present invention

[0040] Inoculate Bacillus cereus with a preservation number of CGMCC No.7979 in NA liquid medium with a pH value of 6-8, shake and culture at 28°C-35°C, 160-220 rpm for 36 hours, and centrifuge the bacteria , resuspended with sterile water, then centrifuged, and suspended with an equal volume of sterile water to obtain a bacterial suspension; wherein, the formula of the NA liquid medium is: beef extract 5g / L, peptone 10g / L, sodium chloride 5g / L L, the balance is water.

[0041] Inoculate the bacterial suspension into the decomposed cow dung (the volume mass ratio of the bacterial suspension to the decomposed cow dung is 2mL:10g) for solid fermentation until the number of Bacillus cereus reaches 10 8 cfu / g or more, during which the solid fermentation temperature is not higher than 50°C, turning once a day to obtain microbial fertilizer. Among them, the mass ratio of organic matter in decom...

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Abstract

The invention relates to the field of microorganisms, and particularly discloses bacillus cereus. The bacillus cereus is collected in CGMCC (China general microbiological culture collection center), and has the collection number being CGMCC No.7979. The bacillus cereus can be stably colonized in the soil, the soil microbial environment is improved, the biological continuous cropping obstacle is eliminated, in addition, an obvious inhibition effect is realized on fusarium oxysporum, in addition, the growth of vanilla can be prompted, and the bacillus cereus can be applied to prevention and treatment of fusarium oxysporum, vanilla growth promotion and preparation of relevant products.

Description

technical field [0001] The invention relates to the field of microbes, more specifically to a bacillus cereus and its application. Background technique [0002] Vanilla planifolia (Vanilla planifolia Andrews) is a tropical vine spice plant of Orchidaceae, and has the reputation of "the king of natural food spices". Its fresh bean pods contain more than 250 kinds of aromatic components after aroma processing, and are widely used in the preparation of various high-end cigarettes, famous wines, and tea leaves. [0003] Vanilla orchid is a perennial crop. With the increase of planting years, the soil microbial flora becomes out of balance, the number of soil-borne pathogenic fungus Fusarium oxysporum increases significantly, soil microbial diversity tends to be simple, and the growth of vanilla orchid is weak. , the problem of continuous cropping biological obstacles has seriously restricted the sustainable development of vanilla orchid planting. In order to reduce the number ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C05F15/00C05F17/00C12R1/085
CPCY02W30/40
Inventor 王辉赵青云赵秋芳王华庄辉发宋应辉朱自慧
Owner 海南兴科热带作物工程技术有限公司
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