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Application of a Curvularia lunata in C14α-Hydroxylation

A kind of Curvularia, hydroxylation technology, applied in the field of biotechnology and steroid drug transformation, can solve the problem of low pharmacological activity

Active Publication Date: 2021-08-06
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, natural steroid drugs often have the problem of low pharmacological activity. The introduction of hydroxyl groups at specific sites of steroid drugs can significantly improve their pharmacological and physiological activities.

Method used

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  • Application of a Curvularia lunata in C14α-Hydroxylation
  • Application of a Curvularia lunata in C14α-Hydroxylation
  • Application of a Curvularia lunata in C14α-Hydroxylation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 Curvularia crescenae CICC40301 transforms the method for 4AD

[0025] The reaction formula of Curvularia crescens transformation 4AD is as follows figure 1 shown.

[0026] Take 200 μL of the Curvularia crescens strain seed solution and connect it to 50 mL of YPD liquid medium, and after cultivating at 28°C for 36 hours, add 0.2% androst-4-ene-3,17-dione substrate (4AD), and then After 48 hours of conversion, the yield of C14α-4AD was 48.3%.

Embodiment 2

[0027] Embodiment 2 Curvularia crescenae CICC40301 transforms the method for 4AD

[0028] Curvularia crescenae CICC40301 was inoculated in YPD liquid medium at an inoculum of 1% after seed cultivation, and after culturing at 25°C for 24 hours, 0.1% androst-4-ene-3,17-dione was added Object (4AD), then transform 24h;

[0029] After 24 hours of transformation, the yield of C14α-4AD reached 41.5%.

Embodiment 3

[0030] Embodiment 3 Curvularia crescenae CICC40301 transforms the method for 4AD

[0031] After Curvularia lunae CICC40301 was cultured by seeds, it was inoculated in YPD liquid medium at an inoculum size of 2%, and after culturing at 30°C for 36 hours, 0.3% of androst-4-ene-3,17-dione was added to it. Object (4AD), then transform 40h;

[0032] After 40 hours of conversion, the yield of C14α-4AD reached 45.2%.

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Abstract

The invention belongs to the technical field of biotechnology and steroid drug conversion, and particularly relates to the application of Curvularia crescenae CICC40301 in C14α-hydroxylation. Curvularia lunata (Curvularia lunata) is used for C11β hydroxylation in industry, and the present invention discovers Curvularia lunata with C14α hydroxylation ability for the first time, and Curvularia lunata CICC40301 can transform 4AD into C14α-4AD, yield Reaching 40%‑50%, this discovery will provide a new route for the C14α hydroxylation of steroids.

Description

Technical field: [0001] The invention belongs to the technical field of biotechnology and steroid drug transformation, and in particular relates to the application of Curvularia crescenae CICC40301 in C14α-hydroxylation. Background technique: [0002] Steroid drugs have a wide range of pharmacological and physiological activities, and play an extremely important role in anti-inflammation, anti-tumor, regulation of sexual function and birth control. For example, the C11α-hydroxylation products of certain steroid compounds are important intermediates for the synthesis of drugs such as dexamethasone and prednisolone; 14α-hydroxyandrost-4-ene-3,6,17-trione has anti-uterine The effect of cancer. However, natural steroid drugs often have the problem of low pharmacological activity, and the introduction of hydroxyl groups at specific sites of steroid drugs can significantly improve their pharmacological and physiological activities. [0003] The structure of steroids is complex a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P33/06C12R1/64
CPCC12P33/06
Inventor 刘晓光李慧杰李改贾龙刚王正祥路福平金鹏毛淑红
Owner TIANJIN UNIV OF SCI & TECH
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