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Bacillus licheniformis, microbial agent prepared by Bacillus licheniformis, biological fertilizer and application thereof

A microbial inoculum, Bacillus technology, applied in the direction of microorganisms, microorganisms, biocides, etc.

Active Publication Date: 2019-07-02
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some strains of this kind of microorganisms are used to make microbial fertilizers or microbial pesticides because of their growth-promoting and antagonistic functions, but they are mainly used in conventional soil production, suitable for moderate to severe saline-alkali land, especially suitable for soil improvement with various obstacles There are still few good bacillus, and it is urgent to strengthen the corresponding research and development work and tap the application potential

Method used

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  • Bacillus licheniformis, microbial agent prepared by Bacillus licheniformis, biological fertilizer and application thereof
  • Bacillus licheniformis, microbial agent prepared by Bacillus licheniformis, biological fertilizer and application thereof
  • Bacillus licheniformis, microbial agent prepared by Bacillus licheniformis, biological fertilizer and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Morphology and identification of Bacillus simplex CGMCC No.16458

[0032] 1. Morphological characteristics of the strain

[0033]Form round, flat, moist and viscous colonies on LB plates, and grow turbidly in LB liquid medium. After Gram staining, it can be seen under the microscope that the ends are blunt and round, of different lengths, Gram-positive bacilli, and there are subterminal spores that are larger than the bacteria.

[0034] 2. Physiological and biochemical characteristics of the strain

[0035] Experiments confirmed that the strain could grow on LB plate with 10% salt content. It was found through experiments that the strain has the ability to dissolve phosphorus, fix nitrogen, produce siderophiles, ACCD and cytokinins. ACCD enzyme activity reached 7.27mM / mg / h.

[0036] 3. Strain identification and preservation

[0037] Through 16S rDNA sequence analysis, comprehensive bacterial morphology, physiological and biochemical characteristics and 16S rDNA gen...

Embodiment 2

[0039] Preparation of CGMCC No.16458 Biological Fertilizer Containing Bacillus simplex

[0040] Inoculate the Bacillus simplex (Bacillus simplex) CGMCC No.16458 bacteria liquid stored in the glycerol tube on the LB solid medium for activation, culture at 30°C for 2 days, inoculate the single colony into the seed medium, 30°C, 200 rpm Minutes, culture hours to the late logarithmic period, the bacterial liquid obtained after the inoculation amount of 1% by mass ratio is transferred once more is the seed liquid. Fermentation is carried out with this seed liquor. 60% of the volume of the fermenter is put into the sterilized fermentation medium, the inoculation amount of the seed liquid is 5%, the temperature is 30°C, and the ventilation rate is 0.5m 3 / h, stirring speed 200 rev / min, dissolved oxygen 75%, defoaming edible soybean oil, cultivated for 45 hours, so that the number of bacteria reached 10 10 cfu / mL.

[0041] The fermentation medium contains the following components: ...

Embodiment 3

[0044] Bacillus simplex (Bacillus simplex) CGMCC No.16458 test against Pythium, Fusarium, Curvularia, Plaque and Phytophthora

[0045] The center of the LB plate was inoculated with the test fungal block, and four small filter paper sheets with a diameter of 6 mm were symmetrically placed 2.5 cm away from the center of the plate, and 10 μL of Bacillus simplex (Bacillus simplex) CGMCC No. Each treatment was repeated 3 times and cultured at 30°C; as a control, the center of the LB plate was inoculated with the test fungal block, and 4 pieces of small filter paper with a diameter of 6 mm were symmetrically placed at a distance of 2.5 cm from the center of the plate, and 10 μL of sterile water was added dropwise to each piece of paper. , each treatment was repeated 3 times and cultured at 30°C. When the fungus in the control group covered the plate or reached the size of a paper sheet, the plate was taken out, the diameter of the fungus was measured, and the inhibition rate was ca...

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Abstract

The invention provides a broad-spectrum functional bacteria (Bacillus simplex)CGMCC No.16458, which has high salt tolerance, multi-function repair and continuous cropping, and salt and alkali resistance for soil resistance. The strain has high ability to dissolve phosphorus, fix nitrogen, produce ferritin, ACCD and cytokinin, and has a salt tolerance of 10%, and has strong antagonistic effects forPythium, Fusarium, Curvularia lunata, Bipolaria maydis and Phytophthora, is suitable for the production of the biological fertilizer with disease prevention, growth promotion, salt and alkali resistance, and the like, and is applied to medium and heavy saline-alkali land production and forage cultivation, as well as from production of vegetables for many years of continuous cropping facilities, the strain promotes emergence, promotes root development, prevents root diseases, improves crop yield and quality, can reduce the use of chemical fertilizers and pesticides, and has broad application prospects.

Description

technical field [0001] The invention relates to microbial strains and applications thereof, in particular to a simple bacillus, a microbial agent prepared by using the bacillus, biological fertilizer and its application. Background technique [0002] With the development of society and the increase of population, the demand for cultivated land resources is increasing. my country has 99.13 million hectares of saline-alkali land, including 700,000 hectares around the Bohai Sea, which is an important potential arable land resource. Saline-alkali land is divided into mild saline-alkali land, moderate saline-alkali land and heavy saline-alkali land. The pH value of mild saline-alkali land is 7.1-8.5, with a salt content of <0.3%, and the plant emergence rate is usually 70%-80%; The value is above 9.5, the salt content is ≥0.6%, and the emergence rate is lower than 50%. Due to unreasonable tillage and fertilization, vegetable fields are generally secondary salinized and soil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P21/00A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12R2001/07C12N1/205A01N63/10
Inventor 王占武彭杰丽胡栋张翠绵贾楠马佳
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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