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A deep-sea fungus Cladosporium and its extract and application

The technology of Cladosporium and its extract is applied in the application field of preparing anti-pathogenic fungi medicine, and achieves the effect of broad-spectrum anti-pathogenic fungi activity and good application prospect.

Inactive Publication Date: 2016-06-22
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The ocean accounts for about 71% of the earth's surface area and is rich in microbial resources, among which microorganisms account for 90% of the number of marine organisms and control all important biological and biochemical cycles that maintain the balance of the earth's ecosystem. Existing research data show that the marine microbial resource bank It is far larger than the terrestrial microbial resource pool, and has not yet been fully explored and utilized by humans. It can be seen that marine microorganisms are rich biological resources for human medicine.

Method used

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  • A deep-sea fungus Cladosporium and its extract and application
  • A deep-sea fungus Cladosporium and its extract and application
  • A deep-sea fungus Cladosporium and its extract and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation and identification of deep-sea fungus Cladosporium sp. FS86

[0029]The deep-sea fungus Cladosporium sp. FS86 was isolated and purified from the sediments at a depth of 1598 m in the South China Sea (12°58.551'E, 17°0.646'N). The separation and purification method is as follows: the sea mud sample is diluted to 10% with sterile water containing 3% crude sea salt, shaken at 27°C for 20 minutes, 0.2 mL is uniformly spread on the separation medium, and cultured in a constant temperature box at 26-28°C. After the colonies grow, pick the colonies for streak purification. Each liter of the separation medium is obtained by: boiling 200g of potatoes for 20min with water, filtering to calculate, then adding 20g of glucose, KH 2 PO 4 3g, MgSO 4 0.75g, B110mg, crude sea salt 30g agar 20g, make up to 1L with water, pH is natural.

[0030] Through morphological study and according to "Fungi Identification Manual" (Wei Jingchao, 1979), the strain was initiall...

Embodiment 2

[0036] Example 2: Preparation of deep-sea fungus Cladosporium sp. FS86 fermentation extract

[0037] Cladosporium FS86 was inoculated into the fermentation medium and cultured at 120 r / min at 26°C for 7 d to obtain Cladosporium FS86 fermentation culture.

[0038] The fermented culture of Cladosporium FS86 was cold-soaked with ethyl acetate for 24 hours, and the cold soak was concentrated under reduced pressure to obtain a brown paste-like extract, which was the fermented extract of Cladosporium FS86.

[0039] The fermentation medium per liter is obtained by: boiling 200g of potatoes for 20min with water, filtering to measure, then adding 10g of glucose, 20g of mannitol, 3g of yeast extract, 5g of peptone, 5g of monosodium glutamate, 3.0g of coarse sea salt, and making up to 1L with water , pH: natural.

Embodiment 3

[0040] Example 3: Antipathogenic fungi activity of deep-sea fungus Cladosporium sp. FS86 fermentation extract

[0041] 1. Determination of antibacterial activity:

[0042] The antibacterial activity of Cladosporium FS86 fermented extract against Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletosporum anthracis, Bispora styrexus or Curvus crescentus was determined by filter paper method.

[0043] The Cladosporium FS86 fermentation extract obtained in Example 2 was dissolved in DMSO and diluted to a concentration of 50 mg / mL.

[0044] The above-mentioned pathogenic fungal bacterial liquid obtained by liquid fermentation is diluted with physiological saline to a concentration of 106 / mL of bacterial count or spore count, and 1 mL of bacterial or spore suspension with a concentration of 106 / mL is drawn into a petri dish, Pour into the PDA medium that has been cooled to an appropriate temperature, mix well, use sterile tweezers to pick up a filter...

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Abstract

The invention discloses a deep-sea fungus Cladosporium and its extract and application. The deep-sea fungus Cladosporium sp. FS86 was preserved in the China Center for Type Culture Collection (CCTCC) on March 27, 2014, address: Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2014108. Its fermented extract To pathogenic fungi Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletotrichum gloeosporioides, Cylindrocladium scoparium ) or Curvularia lunata had significant inhibitory effect. Therefore, the realization of the present invention provides a scientific basis for the development of new anti-pathogenic fungal drugs and the exploration of natural active substances derived from deep-sea fungi.

Description

Technical field: [0001] The invention belongs to the technical field of biology and medicine, and in particular relates to a deep-sea fungus Cladosporium sp. FS86, and a fermented extract prepared from the liquid culture product of the strain, and the extract is used in the preparation of anti-pathogenic fungi medicines. applications in . Background technique: [0002] Candida albicans (Candida albicans) is an important opportunistic pathogenic fungus, which usually exists in the oral cavity, upper respiratory tract, intestinal tract and vagina of normal people. If the defense power is decreased or the interaction of normal flora is out of balance, the bacteria multiply and change the growth form (blastoma hyphal phase) to invade the cells and cause diseases. In recent years, with the application of high-dose antibiotics, hormones, immunosuppressants, and the development of organ transplantation, deep fungal infections are increasing and can be life-threatening. Candida al...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A61K36/06A61P31/10C12R1/645
Inventor 李浩华章卫民王磊谭国慧
Owner GUANGDONG INST OF MICROORGANISM
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