Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs

A technique for poplar gray spot and streptomyces, which is applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., and can solve the problems of unsafe chemical pesticides and few types of microbial pesticides.

Inactive Publication Date: 2014-11-12
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the technical problems of unsafe chemical pesticides and fewer types of microbial source pesticides, thereby providing a preparation method of Streptomyces fermented liquid for preventing and treating poplar gray spot bacterium

Method used

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  • Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs
  • Method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs

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Experimental program
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specific Embodiment approach 1

[0016] Specific embodiment one: the preparation method of a kind of streptomyces fermented liquid of the present embodiment is carried out according to the following steps:

[0017] 1. Activation culture of strains: Streptomyces sp. Syjd3 was inoculated on Gaoshi No. 1 solid medium, and cultured at a temperature of 27-29°C for 4-6 days to obtain activated Streptomyces sp. )Syjd3;

[0018] 2. Fermentation broth culture: put 300mL Gaoshi No. 1 liquid medium into a 500mL Erlenmeyer flask, then autoclave at 110-121°C for 20-30min, after cooling, pick 2- 3 Steps of the inoculation loop 1. Activated Streptomyces sp. Syjd3 was inoculated into Gao's No. 1 liquid medium, and then placed in a shaker incubator for cultivation. The shaker temperature was 27-29°C, and the shaker revolutions 180 to 230 rpm, and the fermentation and cultivation time is 7 to 15 days, so that the Streptomyces fermentation broth for preventing and curing poplar gray spot bacteria can be obtained;

[0019] Whe...

specific Embodiment approach 2

[0025] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, the temperature is 28° C. for 4 days. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0026] Specific embodiment 3: The difference between this embodiment and the specific method 1 or 2 is: in step 2, autoclave at 121° C. for 20 minutes. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for preparing streptomyces fermentation liquor for preventing and treating alamo grey speck disease germs, and relates to a preparation method of the fermentation liquor. The method mainly solves the technical problems that chemical pesticides are unsafe, and microorganism sourced pesticide is few in kind. The method comprises the steps that (1) excitation cultivation of strains is carried out, (Streptomyces sp.) Syjd3 is shifted to gause first solid media for cultivation to obtain activated (Streptomyces sp.) Syjd3; (2) the activated (Streptomyces sp.) Syjd3 is inoculated to the gause first solid media, the gause first solid media after the inoculation are put into a shake cultivation box for cultivation, and the streptomyces fermentation liquor for preventing and treating the alamo grey speck disease germs is obtained. The streptomyces fermentation liquor has an inhibition function on the alamo grey speck disease germs, and also has certain inhibition functions on alamo leaf blight germs, fusarium solani, wheat root rot germs, curvularia lunata and rice bakanae pathogen.

Description

technical field [0001] The invention relates to a preparation method of fermented liquid. Background technique [0002] Poplar gray spot is one of the diseases that harm the leaves of poplar caused by Sporocadus populinus (Bres.) Orsenigo, Rodondi & B. Sutton=Coryneum populinum Bres., with a large area of ​​occurrence and serious damage , mainly occurs on the leaves and treetops of young trees and seedlings, causing leaves to fall off early, cadres canker, young shoots withered, and growth is reduced. In severe cases, it can cause plant death and reduce the survival rate of transplanting. Afforestation is a great threat. [0003] Plant diseases are one of the important factors that threaten agricultural production. Currently, the use of chemical pesticides is the main method to control plant diseases. However, the long-term use of chemical pesticides not only pollutes the environment and destroys the ecological balance, but also the residues are harmful to humans and animal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/06A01N63/02A01P3/00C12R1/465
Inventor 刘雪峰刘雪英马玲张静石凤梅
Owner NORTHEAST FORESTRY UNIVERSITY
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