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Cladosporium sp. strain, and extract and application thereof

A Cladosporium, extract technology, applied in the field of application in the preparation of anti-pathogenic fungal drugs, to achieve the effect of broad-spectrum anti-pathogenic fungal activity and good application prospects

Inactive Publication Date: 2014-09-10
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The ocean accounts for about 71% of the earth's surface area and is rich in microbial resources, among which microorganisms account for 90% of the number of marine organisms and control all important biological and biochemical cycles that maintain the balance of the earth's ecosystem. Existing research data show that the marine microbial resource bank It is far larger than the terrestrial microbial resource pool, and has not yet been fully explored and utilized by humans. It can be seen that marine microorganisms are rich biological resources for human medicine.

Method used

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  • Cladosporium sp. strain, and extract and application thereof
  • Cladosporium sp. strain, and extract and application thereof
  • Cladosporium sp. strain, and extract and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: Isolation and identification of deep-sea fungus Cladosporium sp. FS86

[0029]The deep-sea fungus Cladosporium sp. FS86 was isolated and purified from the sediments at the depth of 1598m in the South China Sea (12°58.551′E, 17°0.646′N). The separation and purification method is as follows: dilute the sea mud sample to 10% with sterile water containing 3% coarse sea salt, shake at 27°C for 20 minutes, take 0.2mL and evenly spread it in the separation medium, and cultivate it in a constant temperature box at 26-28°C. After the colonies grow out, the colonies are picked and streaked for purification. Each liter of the separation medium is obtained in the following manner: boil 200g of potatoes with water for 20min, filter to obtain a meter, then add 20g of glucose, KH 2 PO 4 3g, MgSO 4 0.75g, B110mg, coarse sea salt 30g agar 20g, make up to 1L with water, pH natural.

[0030] Through morphological studies, and according to the "Handbook of Fungal Identifi...

Embodiment 2

[0036] Embodiment 2: Preparation of deep-sea fungus Cladosporium sp. (Cladosporium sp.) FS86 fermentation extract

[0037] Cladosporium FS86 was inoculated into the fermentation medium, cultured at 120 r / min, 26° C. for 7 days, to obtain the cladosporium FS86 fermentation culture.

[0038] The fermentation culture of Cladosporium FS86 was cold-soaked with ethyl acetate for 24 hours, and the cold soak was concentrated under reduced pressure to obtain a brown paste-like extract, which was the fermented extract of Cladosporium FS86.

[0039] The fermentation medium per liter is obtained in the following way: boil 200g of potatoes with water for 20min, filter to obtain a meter, then add 10g of glucose, 20g of mannitol, 3g of yeast extract, 5g of peptone, 5g of monosodium glutamate, 3.0g of coarse sea salt, and make up to 1L with water , pH: natural.

Embodiment 3

[0040] Embodiment 3: Antipathogenic fungal activity of deep-sea fungus Cladosporium sp. (Cladosporium sp.) FS86 fermentation extract

[0041] 1. Determination of antibacterial activity:

[0042] The antibacterial activity of Cladosporium FS86 fermented extracts against Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Gluespora anthracnose, Bispora styloidae or Curvularia lunenee was determined by filter paper method.

[0043] The Cladosporium FS86 fermented extract obtained in Example 2 was dissolved with DMSO and diluted to a concentration of 50 mg / mL.

[0044] Dilute the above-mentioned pathogenic fungus liquid obtained by liquid fermentation with physiological saline to a concentration of 106 / mL of bacteria or spores, and draw 1 mL of bacteria or spore suspension with a concentration of 106 / mL in a petri dish, Pour into the PDA medium that has been cooled to a suitable temperature, mix evenly, use sterile tweezers to pick up a filter paper sheet...

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Abstract

The invention discloses a Cladosporium sp. strain, and an extract and application thereof. The Cladosporium sp. FS86 is collected to China Center for Type Culture Collection (CCTCC) on March 27th, 2014, wherein the address is Wuhan University, Wuhan City, PRC, and the collection number is CCTCC NO: M2014108. The fermentation extract has obvious inhibitory effects on Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletotrichum gloeosporioides, Cylindrocladium scoparium or Curvularia lunata. Therefore, the invention provides scientific references for developing new anti-pathogenic fungus drugs and exploring deep-sea-fungus-derived natural active substances.

Description

Technical field: [0001] The invention belongs to the technical field of biology and medicine, and in particular relates to a strain of deep-sea fungus Cladosporium sp. FS86, a fermented extract prepared from the liquid culture product of the strain, and the use of the extract in preparing anti-pathogenic fungal application in medicine. Background technique: [0002] Candida albicans (Candida albicans) is an important conditional pathogenic fungus, which usually exists in the oral cavity, upper respiratory tract, intestinal tract and vagina of normal people. If the general defense force is reduced or the mutual restriction of normal flora is out of balance, the bacteria will multiply and change the growth form (sprout mycelium phase) to invade cells and cause diseases. In recent years, with the application of large doses of antibiotics, hormones, immunosuppressants, and the development of organ transplantation, deep fungal infections are increasing and can be life-threatenin...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61K36/06A61P31/10C12R1/645
Inventor 李浩华章卫民王磊谭国慧
Owner GUANGDONG INST OF MICROORGANISM
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