46 results about "Cladosporium sp" patented technology

This invention has clone Stilbene synthase gene (sts) from Parthenocissi Tricuspidatae endogeny eumycete, the coding sequence similarity between Stilbene synthase gene and Parthenocissi Tricuspidatae is 95.25%,this eumycete belongs to Cladosporium sp by 18SrDNA series assessment. High performance liquid chromatogram and mass chromatographic analysis indicate that this eumycete can also composite resveratrol by culture in vitro. The analysis about non- coding part of endogeny eumycete sts gene indicates that: it is very different from regulatory element of sts gene which is in plants. Importing exogenous gene(such as VHb,ipt and iaaM and so on ) in endogeny eumycete can promote endogeny eumycete growing.

The invention belongs to the technical field of biology. Lipase widely exists in animal tissues, plants and microorganisms and can catalyze hydrolysis, alcoholysis, esterification and ester interchange of ester compounds, synthesis of compounds and the like. The invention aims to provide cladosporium capable of producing organic solvent tolerant lipase, which is a cladosporium strain (Cladosporium sp.) WBRD3.10062425 and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 4962. The invention also aims to provide the organic solvent tolerant lipase produced by the strain and a preparation method thereof. The lipase produced by the strain disclosed by the invention is the organic solvent tolerant lipase, has strong tolerance for methanol, ethanol and acetone and has wide application value in various industrial fields of biodiesel, medical industry, foods, oleochemical industry, environmental protection, detergents, textile, papermaking and the like.

The invention discloses a chlorpyrifos pesticide-degrading bacterium which refers to a Hu-01strain pertaining to Cladosporium sp. The preservation number of the strain is CCTCC M207111, and a Genebank accession number of the ITS sequence analysis is EF405864. Meantime, the invention discloses a method of preparation of inocula. The invention has the advantages of low production cost of inocula, convenient operation and good degradation effect. The invention is suitable for being widely used in fruit and vegetable production and export bases in China or in places possessing green food trademark, which has great significances in promoting the production of uncontaminated vegetable and green food.

The invention provides application of endophytic cladosporium fungus of liriope spicata var.prolifera Y.T.MA in preparing steroid saponin, and relates to the technical field of microorganisms. The application is mainly used for solving the problems that most existing steroid saponin of liriope spicata var.prolifera Y.T.MA is from extracts of liriope spicata var.prolifera Y.T.MA, tubers of liriopespicata var.prolifera Y.T.MA are long in production period, low in steroid saponin content and high in saponin extraction cost, and accordingly the market volume of the steroid saponin of liriope spicata var.prolifera Y.T.MA is limited. The fungus is separated from living tubers of liriope spicata var.prolifera Y.T.MA, authenticated as Cladosporium sp.EF111 by means of the 18 S rDNA sequence, andpreserved in the China Center for Type Culture Collection. The preservation date is December 7th, 2016, and the preservation number of CCTCC No:M2016725. According to the application, liriope spicatavar.prolifera Y.T.MA produced by a liriope spicata var.prolifera Y.T.MA GAP base which is the original production place of liriope spicata var.prolifera Y.T.MA in Oumiao town, Xiangyang city in Hubeiprovince, endophytic fungus strain liquid of liriope spicata var.prolifera Y.T.MA is used for fermentation, and the steroid saponin of liriope spicata var.prolifera Y.T.MA is produced. The endophyticfungus is an important microorganism for finding new resources of steroid saponin of liriope spicata var.prolifera Y.T.MA.

The invention provides cladosporium sp. of CGMCC (China General Microbiological Culture Collection) No. 5782. The invention also provides phyllobacterium myrsinacearum of CGMCC No. 5783. The invention also provides a microbial compound bacterium agent containing the two strains for treating an oilfield fracturing flowback fluid, and a preparation method of the microbial compound agent. The two strains are respectively put in two specific culture media for shaking culture, and two strains are respectively inoculated into two reactors filled with the specific culture media according to the inoculation amount of 5% to 15%, and are continuously cultured for 8 to 48 hours to obtain a liquid bacterium; and then the cultured liquid bacterium is compounded according to the volume ratio of cladosporium sp. to phyllobacterium myrsinacearum of 1 to 5:1 to obtain the microbial compound bacterium agent. The invention can effectively solve the problem that macromolecular organic compounds in the oilfield fracturing flowback liquid is difficult to biodegrade, and reduces secondary pollution of the environment.

The invention discloses a cladosporium sp. and application thereof in degradation of polyurethane plastics. The cladosporium sp. is classified and named as Cladosporium sp., the strain name is P7, thecladosporium sp. is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the strain is CCTCC NO: M 2020627, and the preservation date is October 23, 2020. The strain P7 adopted by the invention can grow by taking Impranil DLN as a unique carbon source, and the degradation rate of the waterborne polyurethane Impranil DLN reaches 95.8% within 7 days. In addition, the strain has a relatively high degradation effect on polyurethane foam, and 86.6% of polyurethane foam can be degraded within 15 days. Therefore, the strain provided by the invention has an extremely important application value in biological treatment of plastic wastes.

The invention discloses a Cladosporium sp. strain, and an extract and application thereof. The Cladosporium sp. FS86 is collected to China Center for Type Culture Collection (CCTCC) on March 27th, 2014, wherein the address is Wuhan University, Wuhan City, PRC, and the collection number is CCTCC NO: M2014108. The fermentation extract has obvious inhibitory effects on Candida albicans, Aspergillus niger, Aspergillus flavus, Trichoderma viride, Colletotrichum gloeosporioides, Cylindrocladium scoparium or Curvularia lunata. Therefore, the invention provides scientific references for developing new anti-pathogenic fungus drugs and exploring deep-sea-fungus-derived natural active substances.

The invention provides a lactobacillus plantarum PC28152 with broad-spectrum fungal inhibition activity. The strain is preserved in China General Microbiological Culture Collection Center on July 6, 2020, and the preservation number is CGMCC No.20198. The lactobacillus plantarum PC28152 and the fermentation liquor thereof have the effect of inhibiting fungal activity in a broad spectrum, and particularly have an obvious inhibiting effect on aspergillus flavus, penicillium chrysogenum, penicillium expansum, fusarium moniliforme and cladosporium cladosporioides. When the strain is applied to food preservation and preservation, infection of spoilage fungi to food can be effectively inhibited, and the shelf life of the food is remarkably prolonged.

The invention belongs to the field of microbial biotechnology, relates to a (cladosporium cladosporioides) Cc-106 strain for production of lovastatin through liquid fermentation and application and a fermentation production method. The Cc-106 strain is preserved in China Center for Type Culture Collection on July, 7, 2016 with the preservation number CCTCC M 2016380. The strain has the good characteristics of high yield production of the lovastatin, high sporulation quantity, simple culturing and the like. Under the conditions that the initial pH of a fermentation medium is 5.5, the culture temperature is 28 DEG C, and Cc-106 strain liquid fermentation is performed for 80h to 136h in a 5L fermentation tank, the yield of the lovastatin can reach about 184mg / L.

A alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6380, and the name of the strain is 11775-42-Z1, and is 42# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 42# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 42# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.

The invention relates to a method for biologically synthesizing crude extracts of theaflavins with cladosporium tenuissimum, which comprises following steps: obtaining high-producing strain cladosporium tenuissimum of polyphenol oxidase through screening a large amount of microbes, culturing the strains to synthesize polyphenol oxidase in optimized fermentation medium under the constant temperature and under the condition that the pH value is 4.5-5.3, wherein the rotation speed is 110-130r / min and the temperature is 22-25DEG C, obtaining crude enzyme liquid of the polyphenol oxidase, and inputting oxygen to simulate oxidation catechin for 40-50 minutes in vitro to prepare the theaflavins. The theaflavins content which is higher than 20.00% in raw products of the theaflavins can be obtained through applying the method of the invention, the process steps are simple, the equipment investment is little, the working efficiency is high, the production cost is low, the method does not use any organic solution in the reaction process, and the method is a green environmental protection theaflavins producing method.

The invention discloses a cladosporium yielding cholesterase (Cladosporium sp.) DZ16 which is screened, wherein the preservation number is CCTCC M2016685. The bacterial colony of the culture is velvet-like and is activated by means of a first step of preserving and activating the culture and a second step of plate spraying of the culture. In the early stage of fermentation, the biomass of the bacterial strain is activated and cultured in a low salt liquid state in the first step. In the later stage, by means of multifactor compounding action in the second step, the bacterial strain is inducedtop secrete zymoprotein, so that the enzyme activity is improved. The cholesterase produced by fermenting the bacterial strain has a good application potential in the fields of medical detection, food, pulping and paper-making industry, environmental protection and the like.

The invention relates to a method for biologically synthesizing crude extracts of theaflavins with cladosporium tenuissimum, which comprises following steps: obtaining high-producing strain cladosporium tenuissimum of polyphenol oxidase through screening a large amount of microbes, culturing the strains to synthesize polyphenol oxidase in optimized fermentation medium under the constant temperature and under the condition that the pH value is 4.5-5.6, wherein the rotation speed is 110-130r / min and the temperature is 22-25 DEG C., obtaining crude enzyme liquid of the polyphenol oxidase, and inputting oxygen to simulate oxidation catechin for 40-50 minutes in vitro to prepare the theaflavins. The theaflavins content which is higher than 20.00% in raw products of the theaflavins can be obtained through applying the method of the invention, the process steps are simple, the equipment investment is little, the working efficiency is high, the production cost is low, the method does not use any organic solution in the reaction process, and the method is a green environmental protection theaflavins producing method.

The invention discloses Cladosporium Sp. XJ-AC03 which is preserved in China center for type culture collection under the number of CCTCC NO.M2011483 on December 26 2011. A bacterial colony of the Cladosporium Sp. XJ-AC03 is circular with white regular edge. The bacterial colony is in milk white early in culture, and gradually turns gray to deep grayish green over time, and the center of the bacterial colony project slightly. Mycelia of the Cladosporium Sp. XJ-AC03 have transverse septa without longitudinal septa and are dense. Certain mycelia have conidiophore which is branched. The conidiophore is olivary with brown to dark brown color and is concentrated with 0-3 septa. The Cladosporium Sp. XJ-AC03 is obtained from root of Xinjiang Aconitum leucostomum by artificial separating and screening. The Cladosporium Sp. XJ-AC03 of aconitine can be synthesized effectively, and can be used as material for preparing antineoplastic drugs, anti-inflammatory drugs, and anti-rheumatic drugs.

The purpose of the present invention is to provide a hypochlorous acid aqueous solution having excellent antimicrobial effect and safety. An antimicrobial agent is provided which is a hypochlorous acid aqueous solution with a pH of 3.0-6.7, wherein the effective chlorine concentration in the aqueous solution is 50-260 ppm. Further, a sterilization method is provided characterized by immersing themember to undergo sterilization treatment in the aforementioned antimicrobial agent for 0.5-10 minutes. The antimicrobial agent has a broad antimicrobial spectrum of noroviruses, Staphylococcus aureusand other bacteria, yeast belonging to Rhodotorula, and Cladosporium cladosporioides and other fungi.

The invention relates to a multifunctional plant conditioning matrix. The multifunctional plant conditioning matrix comprises, by weight, 20 to 300 parts of a rooting agent, 10 to 70 parts of a water-retaining agent, 1000 to 2000 parts of soil, and 10 to 50 parts of a heavy metal ion adsorbent. The heavy metal ion adsorbent comprises orange peel, corn, and bran which are taken as base materials, and a mixed bacterial agent which is taken as a modifier and comprises beer yeast, Gordon amarae, aspergillus oryzae, cladosporium sp, and rhizopus nigricans. Beneficial effects are that: organic combination of agricultural crop with bacteria is realized; heavy metal ions can be absorbed effectively; water retention, slow release, fertilizer retention, soil improvement, and biological activation effects are achieved; transplanted plant survival rate is increased, and soil structure is improved at the same time; the ingredients of the multifunctional plant conditioning matrix are degradable organic matters, so that environmental pollution is not caused.

The invention discloses a fungus with manganese oxidation capacity, the fungus can oxidize Mn < 2 + > in a water body to generate manganese oxide insoluble in water, the Mn < 2 + > oxidizing fungus is Cladosporidium sp. XM01, and the preservation number is CGMCC NO.21083. The Cladosporium sp. XM01 strain is used for Mn < 2 + > oxidation in natural water, the operation temperature is stable (15-30 DEG C) and the pH value (6.0-7.5) is in a neutral range, so that the cladosporium XM01 strain has very high Mn < 2 + > oxidation efficiency; in-situ remediation of water and soil polluted by heavy metals or trace organic matters can be realized by adopting XM01 circulating the Mn (II); the manganese oxide generated by oxidation in the growth process of the strain has good application potential in the fields of sewage treatment, water environment remediation, soil and the like.

A cladosporium sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6608, and the name of the strain is 11673-58, and is 58# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 58# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 58# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.

The invention provides a cladosporium fulvum genetically engineered bacterium and application of the cladosporium fulvum genetically engineered bacterium in production of hypericin and sperm, and belongs to the technical field of biological pharmacy. The cladosporium fulvum genetically engineered bacterium is obtained by replacing a trihydroxy naphthol reductase gene in cladosporium fulvum with a cytochrome P450 gene. The invention also provides a method for producing the hypericin and the eriosperm by adopting the cladosporium fulvum genetically engineered bacterium, and the method comprises the step of carrying out fermentation culture by adopting the cladosporium fulvum genetically engineered bacterium to obtain the hypericin and the eriosperm. The cladosporium fulvum genetically engineered bacterium disclosed by the invention can be used for preparing hypericin and lysozyme through a fermentation method, and is efficient and environment-friendly.

The invention provides an AchE (acetylcholinesterase) inhibiting secondary metabolite of endophytic cladosporium sp. Pt-2 of portunus trituberculatus and a preparation method thereof, belonging to the technical field of biology. The preparation method comprises the following steps of: (1) carrying out liquid fermentation on endophytic cladosporium sp. Pt-2 of portunus trituberculatus taken as a strain to obtain a fermented product; (2) carrying out vacuum concentration on fermentation liquor at 40 DEG C until the volume of the fermentation liquor is 1 / 3 of the original volume, then extracting the fermentation liquor with ethyl acetate and concentrating extract liquor until the extract liquor is dry; and (3) carrying out silica column chromatography on the organic phase extract, which has been concentrated until the extract is dry, of the cladosporium sp. Pt-2 fermentation product, carrying out gradient elution with a chloroform / methanol system to obtain six polarity segments and separating the segment with the highest activity by adopting a C18 reversed-phase column and sephadex chromatography, thus obtaining the secondary metabolite with AchE inhibition ratio up to 70-90%. The secondary metabolite and the preparation method have the advantages that the defects that current drugs are expensive and have side effects and high chemical synthesis difficulty are overcome; and the new drug and preparation method are provided to provide theoretical bases for development of senile dementia preventing biological drugs.

The invention provides a phyllobacterium myrsinacearum which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No: 5783. Cladosporium sp. which has a preservation number of CGMCC No: 5782 and the phyllobacterium myrsinacearum strain are respectively put into two culture media to carry out shake cultivation, then respectively inoculated to two reactors of the specific culture media according to the inoculation size of 5-15 percent by volume and continuously cultured for 8-48 hours to obtain bacteria liquid, and then the well-cultured bacteria liquid is compounded to obtain a compound microbial agent. Microorganisms are constantly suitable for the growth environment of oilfield wastewater through growth, propagation and domestication of the microorganisms in fracturing fluid flowback liquid, and finally, the purpose of degrading the fracturing fluid flowback liquid to the chemical oxygen demand of is achieved. The phyllobacterium myrsinacearum can be used for effectively degrading macromolecules and organic matters in the fracturing fluid flowback liquid, the degradation rate and the water clarity are high, and secondary pollution to the water body environment and the soil environment is reduced.

The invention relates to cladosporium tenuissimum as the fungi with the probiotic effect to panax notoginseng and the capability of increasing the content of notoginsenoside as well as an inoculant ofthe cladosporium tenuissimum and belongs to the technical field of biology. The bacterial strain is separated from stems of healthy biennial panax notoginseng, is authenticated to be the cladosporiumtenuissimum and is preserved in China General Microbiological Culture Collection Center with a preservation number being CGMCC No.14126. Preparation of the inoculant mainly comprises the following steps of pure culture of microorganisms, preparation of a spore suspension, production of the inoculant and application of the inoculant. The inoculant can also be used by being mixed with an organic fertilizer to prepare a bacterial fertilizer, has the probiotic effect to the panax notoginseng, can promote increase of the content of the notoginsenoside to a certain extent and shows a good ecological application prospect.