Cc-106 (cladosporium cladosporioides) strain for high-yield production of lovastatin and application thereof

A technology of Cladosporium cladoides and lovastatin, which is applied in the field of microbial biology, can solve the problems of lack of excellent industrial strains of Lovastatin, and achieve the effects of enriching strains, simple cultivation, and large spore production

Inactive Publication Date: 2017-05-31
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In response to the increasing demand for Lovastatin in the market

Method used

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  • Cc-106 (cladosporium cladosporioides) strain for high-yield production of lovastatin and application thereof
  • Cc-106 (cladosporium cladosporioides) strain for high-yield production of lovastatin and application thereof
  • Cc-106 (cladosporium cladosporioides) strain for high-yield production of lovastatin and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Culture medium embodiment 1 high-yield Lovastatin Cladosporium ( Cladosporium cladosporioides ) Isolation, screening and identification of Cc-106 strain

[0023] ① PDA medium: 20% potato, 2% glucose, 2.0% agar powder, pH 5.5~6.0. Used for isolation, purification and identification of mold strains.

[0024] ② PD culture medium: potato 20%, glucose 2%, beef extract 0.5%, pH 5.5~6.0. It is used for the screening of high-yield lovastatin mold strains.

[0025] experimental method

[0026] 2.1 Isolation and purification of mold strains

[0027] Pick a small amount of mycelia from the surface of samples collected from different habitats (food, soil, fruit, organic matter, etc.) and insert them on the surface of the PDA medium plate, culture at 28°C for 24 hours, and after the white fluffy mycelium grows, take a little mycelium After transferring to another PDA medium plate and continuing to culture for 3 days to produce spores, the typical characteristics of mold were ob...

Embodiment 2

[0039] Embodiment 2 Cladosporium ( Cladosporium cladosporioides ) Growth curve of Cc-106 strain in 5 L fermenter and production rule of Lovastatin

[0040] 1 strain: Cladosporium ( Cladosporium cladosporioides ) Cc-106 strain.

[0041] culture medium

[0042] ① PDA medium: potato 20%, glucose 2%, agar powder 2.0%, pH 5.0~5.5. For the activation culture of Cc-106 strain.

[0043] ② PD culture medium: potato 20%, glucose 2%, pH 5.0~5.5. Used for primary seed culture of the Cc-106 strain.

[0044] ③ Secondary seed culture solution: 20% potato, 2% glucose, 0.5% beef extract, pH 5.0~5.5. Used for secondary seed culture of the Cc-106 strain.

[0045] ④ Fermentation medium: 20% potato, 2% glucose, 0.5% yeast extract, 1.5% peptone, 0.2% NaCl, pH5.0~5.5. Used for the fermentation of Cc-106 strain to produce Lovastatin.

[0046] experimental method

[0047] 3.1 Cultivation of Cc-106 strain

[0048] Pick a small amount of Cc-106 strain mycelium with an inoculation needle, tran...

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Abstract

The invention belongs to the field of microbial biotechnology, relates to a (cladosporium cladosporioides) Cc-106 strain for production of lovastatin through liquid fermentation and application and a fermentation production method. The Cc-106 strain is preserved in China Center for Type Culture Collection on July, 7, 2016 with the preservation number CCTCC M 2016380. The strain has the good characteristics of high yield production of the lovastatin, high sporulation quantity, simple culturing and the like. Under the conditions that the initial pH of a fermentation medium is 5.5, the culture temperature is 28 DEG C, and Cc-106 strain liquid fermentation is performed for 80h to 136h in a 5L fermentation tank, the yield of the lovastatin can reach about 184mg/L.

Description

technical field [0001] The invention belongs to the field of microbial biotechnology, and relates to a strain of Cladosporium ( Cladosporium cladosporioides ) Cc-106 strain, application and fermentation production method. Background technique [0002] Lovastatin is recognized as an ideal drug for lowering cholesterol in various countries. It has the characteristics of high efficiency, low toxicity and safety. It can selectively inhibit the activity of hydroxymethylglutaric acid coenzyme A (HMG-CoA) reductase and block the biosynthesis of cholesterol. In recent years, it has been widely used in clinical treatment. From 1979, Japanese scholar Professor Akira Endo from Monascus red ( Monascus ruber ) isolated an active substance that can strongly inhibit cholesterol synthesis and named it Monacolin K (Monacolin K). After a lapse of 1 year, Albert et al. Aspergillus terrers ) found a similar substance, and named Mevinolin, now known as Lovastatin. Lovastatin has since be...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/06C12R1/645
CPCC12N1/14C12P17/06C12N1/145C12R2001/645
Inventor 蒋冬花许楚旋郑婕施
Owner ZHEJIANG NORMAL UNIVERSITY
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