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Cladosporium Sp. XJ-AC03 and application thereof

A technology of XJ-AC03 and Cladosporium, applied in the field of microorganisms, can solve the problems of lack of aconitine sources and high price

Inactive Publication Date: 2012-10-03
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the source of aconitine is indeed very scarce and expensive, which is far from meeting people's research and clinical medical needs.

Method used

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  • Cladosporium Sp. XJ-AC03 and application thereof
  • Cladosporium Sp. XJ-AC03 and application thereof
  • Cladosporium Sp. XJ-AC03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 The separation of Cladosporium aconitum XJ-AC03

[0023] 1) The roots of Aconitum diphtheriae collected from the Natila prairie in Xinyuan County, Xinjiang Uygur Autonomous Region were washed with deionized water to clean the surface of the sediment, and the surface was disinfected.

[0024] 2) Absorb the water on the surface-sterilized root tissue with sterilized filter paper, then cut it into tissue pieces with a size of 5 mm×5 mm×5 mm, and inoculate them on the surface of PDA solid medium for 20 Cultivate in the dark in an incubator.

[0025] 3) When mycelium grows from the edge of the tissue block, pick the mycelium from the edge of the colony and inoculate it on the surface of the PDA solid medium, and culture it in a dark incubator at 20 °C until it is purified into a single colony; collect the cultured fungal mycelium , extract its alkaloids with organic solvents; use thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) to ...

Embodiment 2

[0026] Example 2 Identification of Cladosporium XJ-AC03

[0027] 1) Morphological features

[0028] Such as figure 1 , figure 2 , image 3 As shown, the colony of this strain is round, with regular white edges; it is milky white at the initial stage of cultivation, and as the cultivation time goes on, the colony gradually turns gray to dark gray-green, and the center is slightly protruding; the mycelium of this strain has Diaphragm, no mediastinum, relatively dense, some hyphae with conidiophores, and conidiophores with branches; conidia olive-shaped, brown to dark brown, concentrated distribution, ranging from 0 to 3 septa.

[0029] 2) Culture characteristics

[0030] The strain grows moderately on PDA solid medium, and the optimum growth temperature is 20 ℃, and the growth is inhibited when the temperature is higher than 28 ℃;

[0031] The components and proportions of the PDA solid medium are: 200 g of potatoes, 20 g of glucose, 17 g of agar, and autoclaved at 121 °C...

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Abstract

The invention discloses Cladosporium Sp. XJ-AC03 which is preserved in China center for type culture collection under the number of CCTCC NO.M2011483 on December 26 2011. A bacterial colony of the Cladosporium Sp. XJ-AC03 is circular with white regular edge. The bacterial colony is in milk white early in culture, and gradually turns gray to deep grayish green over time, and the center of the bacterial colony project slightly. Mycelia of the Cladosporium Sp. XJ-AC03 have transverse septa without longitudinal septa and are dense. Certain mycelia have conidiophore which is branched. The conidiophore is olivary with brown to dark brown color and is concentrated with 0-3 septa. The Cladosporium Sp. XJ-AC03 is obtained from root of Xinjiang Aconitum leucostomum by artificial separating and screening. The Cladosporium Sp. XJ-AC03 of aconitine can be synthesized effectively, and can be used as material for preparing antineoplastic drugs, anti-inflammatory drugs, and anti-rheumatic drugs.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a new Mycobacterium aconitum ( Cladosporium sp. ) and its applications. Background technique [0002] Aconitum is the earliest recorded poisonous plant in my country. The whole body of Aconitum is poisonous, and the root of the genus is the most poisonous. Its toxic components are mainly diterpene alkaloids. The poisoning of Aconitum is mainly caused by accidental ingestion and improper medicinal use. Generally speaking, native animals have the ability to recognize aconitum, and aconitum irritates the oral mucosa, so they will not actively eat. Animals introduced from other places lack the ability to judge poisonous weeds and actively eat and are poisoned. [0003] Among the main components of diphtheria aconitum, aconitine is the most toxic, and aconitine is mainly toxic to the cardiovascular and nerves of animals. Cardiovascular toxicity is mainly to excite the vagus ner...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61K36/06A61K31/439A61P29/00A61P35/00A61P19/02C12R1/645
Inventor 李勤凡杨凯梁洁孔祥雅夏爽
Owner NORTHWEST A & F UNIV