Genetic transformation method of maize curvularia germination conidia

Abstract

The invention relates to a method for genetic transformation of germinating conidiophore of Curvularia lunata Boed. The method comprises the following steps: culturing the germinating conidiophore of the Curvularia lunata Boed as a receptor by a slide suspension method; co-culturing agrobacterium containing an epiphyte conversion vector system and the germinating conidiophore; and screening transformants with resistibility on an antibiotic culture medium. The system uses the Curvularia lunata Boed as initial bacteria, spore is scratched by 0.9 percent of physiological saline after the Curvularia lunata Boed is cultured on the PDA culture medium for 5 to 7 days, and is used for infection of the agrobacterium after the spore is germinated by the slide suspension culture method. The infected germinating conidiophore obtains resistant transformant by co-culture, bacteriostasis culture and anti-selective agent screening culture of continuous two generations, so that a Curvularia lunata Boed mutant base is constructed to provide rich screening strain sources for weak pathogenicity and pathogenic defective Curvularia strain, and meanwhile can be used for cloning related gene of Curvularia lunata Boed pathogenicity so as to lay a foundation for further investigating the pathogenesis mechanism of the Curvularia lunata Boed strain.

Description

technical field

[0001] The invention relates to a method for genetic transformation of germinated conidia of Curvularia maize, in particular to a method for genetic transformation of germinated conidia mediated by Agrobacterium and using the germinated conidia cultured by the slide suspension method as a material to obtain cornbent A method for a spore mutant belongs to the field of biotechnology. Background technique

[0002] Curvularia lunata (Wakker) Boed, caused by Curvularia lunata (Wakker) Boed, has caused major damage in most countries of the world. In 1996, the disease caused severe maize loss in China, and 40% of the maize production areas were infected by the disease. In order to better control the disease and breed resistant varieties, it is of great significance to conduct in-depth research on the pathogenic molecular mechanism of the pathogen. Isolating and identifying pathogenicity-related genes of Curvularia sp. is the key to achieve this goal, and creating ...