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Curvularia lunata strain and application thereof

A technology of Curvularia and Crescent, which is applied in the field of Curvularia crescentus strains, can solve the problems such as the unsuccessful development of biological barnyard control agents, and achieve the effects of low development cost, convenient processing, and low cost

Inactive Publication Date: 2012-05-02
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far commercialized biological barnyard control agent has not been successfully developed

Method used

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  • Curvularia lunata strain and application thereof
  • Curvularia lunata strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, isolation and identification of bacterial strain

[0035] 1. Isolation of strains

[0036] (1) Naturally susceptible barnyardgrass leaves were collected in the wild. (2) remove some pieces of tissue about 4 mm long from the sick and healthy junction of sick leaves, carry out surface disinfection with 2% (v / v) sodium hypochlorite solution for 10 minutes, repeat 3 times; use 65% (v / v) sodium hypochlorite solution afterwards v) Alcohol surface disinfection for 1 minute, repeat 3 times. Finally, rinse with sterile water. These tissues were transferred to PDA plates and placed in a 28°C incubator for cultivation. (3) After single spore separation and purification, pure culture bacteria are obtained.

[0037] 2. Screening of strains

[0038] Leaf segment method: cut off the third leaf of barnyardgrass, cut out the flat 4cm long leaf segment in the middle, and paste it face up on the surface of the water agar (containing 0.05 / L benzimidazole) plate. Wash th...

Embodiment 2

[0050] Embodiment 2, the acquisition of spore and the preparation of spray

[0051] 1. Acquisition, collection and storage of spores

[0052] (1) Method I

[0053] Inoculate Curvularia lunata (Curvularia lunata) B6 CGMCC No.2928 on a PDA plate medium, and culture it for 14 days at 28°C and in the dark. The conidia produced by the strain are on the surface of the medium; The spores grown on the surface of the culture medium were collected and the spore powder was directly used to make barnyardgrass biological control preparations, or stored in a 4°C refrigerator and taken out when needed.

[0054] The composition of the PDA medium: glucose 20g, agar 15g, 200g potato extract, distilled water to 1L. The extract of 200g of potatoes is prepared as follows: Weigh 200g of potatoes, wash, peel and cut into small pieces, add 1000ml of water and boil for half an hour, filter through gauze, and take the filtrate to obtain 200g of potato extract.

[0055] Results: The spore yield can r...

Embodiment 3

[0071] Embodiment 3, the application of biological agent

[0072] 1. Control of barnyardgrass by biological agents

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Abstract

The invention provides a curvularia lunata strain and application thereof. The strain is curvularia lunata B6 of which the preservation number is CGMCC No.2928. The curvularia lunata strain B6 CGMCC No.2928 has strong and fast pathogenicity to echinochloa crusgalli; field experiments show that the echinochloa crusgalli shows spot and dead symptoms in a week after spores of the curvularia lunata strain are sprinkled to the echinochloa crusgalli and the fresh weight inhibition rate to the echinochloa crusgalli can reach 90 percent in 28 days. The fungus strain curvularia lunata strain B6 CGMCC No.2928 is separated from echinochloa crusgallis naturally having diseases in fields without any artificial gene modification; therefore, the curvularia lunata strain cannot have any ecological and environmental hidden danger when released to nature and is an absolutely-safe biological prevention and control medium.

Description

technical field [0001] The invention relates to a strain of Curvularia lunata strain and application thereof. Background technique [0002] Barnyardgrass (Echinoloa crusgalli (L.) Beauv), belonging to the family Gramineae, is distributed in tropical and temperate regions and is ubiquitous throughout the country. It is one of the most serious weed species harmful to crops. Barnyardgrass is one of the vicious weeds in rice fields, which is associated with rice and is extremely difficult to remove. It also occurs in wet early land and harms autumn dry land crops such as cotton and soybean. Typically, as weed density increases, its competitiveness increases and crop yield losses increase. C as a high photosynthetic rate 4 plants, in CO 2 When the concentration is low, barnyardgrass on CO 2 The utilization rate is greater than that of C 3 Plants of rice and soybeans. Strong adaptability, like warm and humid environment, not only can grow in shallow water but also more drou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P13/02C12R1/645
Inventor 倪汉文李静韦韬曹永松王琦
Owner CHINA AGRI UNIV
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