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Streptomyces with inhibiting effect on poplar gray leaf spot pathogen

A poplar gray spot and inhibition technology, applied in the direction of chemicals, bacteria, and fungicides used for biological control, can solve the problems of harm to humans and animals, destruction of ecological balance, and pollution of the environment by chemical pesticides

Inactive Publication Date: 2014-11-05
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

[0003] Plant diseases are one of the important factors that threaten agricultural production. Currently, the use of chemical pesticides is the main method to control plant diseases. However, the long-term use of chemical pesticides not only pollutes the environment and destroys the ecological balance, but also the residues are harmful to humans and animals. Pathogens have developed drug resistance, so the research on biological control of plant diseases has received more and more attention

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  • Streptomyces with inhibiting effect on poplar gray leaf spot pathogen
  • Streptomyces with inhibiting effect on poplar gray leaf spot pathogen
  • Streptomyces with inhibiting effect on poplar gray leaf spot pathogen

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specific Embodiment approach 1

[0016] Specific embodiment one: the streptomyces that this embodiment has inhibitory effect to poplar gray spot bacterium is streptomyces (Streptomyces sp.) Syjd3, is preserved in China Microbiology Culture Collection Management Committee General Microorganism Center (CGMCC), and preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city. The preservation date is June 17, 2014, and the preservation number is CGMCC No.9355.

[0017] In this embodiment, Streptomyces Syjd3 was streaked and cultured on Gao's No. 1 medium. After culturing at 28°C for 7-14 days, the aerial hyphae, basal mycelia and spores on the inserts were observed with an optical microscope and an electron microscope, respectively. morphological features (such as figure 1 ). The mycelium is initially white, and later turns light yellowish brown. The surface of the colony is dry and powdery, and the color of the front and back is the same. Aerial hyphae are abundant a...

specific Embodiment approach 2

[0020] Specific embodiment 2: In this embodiment, Streptomyces sp. Syjd3 is isolated from 3-year-old Populus argentina in the third nursery of Harbin City, Heilongjiang Province. The separation method is carried out as follows:

[0021] (1) 5 trees were randomly selected from the 3-year-old silver poplar in the third nursery of Harbin City, Heilongjiang Province, and 30 leaves were randomly collected from each tree, and the leaves were surface-sterilized with 75% alcohol in an ultra-clean workbench.

[0022] (2) Grind 10 grams of sterilized leaves with a mortar, put them into a triangular flask equipped with glass beads and sterile water, shake them on a shaker at 150 rpm for 30 minutes, and after standing still for 30 seconds, Dilute 10 times, prepare 10 -1 、10 -2 、10 -3 、10 -4 Dilution solution, draw 0.2ml of the dilution solution and add it dropwise on Gao’s No. 1 medium plate, spread evenly, and place the culture dish upside down in an incubator at 28°C.

[0023] Gao'...

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Abstract

The invention discloses streptomyces with an inhibiting effect on poplar gray leaf spot pathogen, relating to a strain of streptomyces. The invention provides the streptomyces which has the inhibiting effect on the poplar gray leaf spot pathogen and provides an effective way for biological prevention and control of poplar gray leaf spot. The streptomyces with the inhibiting effect on the poplar gray leaf spot pathogen is streptomyces Syjd3, and is preserved in the Common microorganism Center of China Committee for Culture Collection of Microorganisms; the preservation address of streptomyces is No. 3, No.1 Institute, west Beichen Road, Chaoyang District, Beijing; the preservation date of streptomyces is June 17, 2014; the preservation number of streptomyces is CGMCC No.9355. Streptomyces Syjd3 disclosed by the invention has the inhibiting effect on the poplar gray leaf spot pathogen, and also has a certain inhibiting effect on poplar stemphylium botryosum, fusarium solani, bipolaris sorokiniana, curvularia lunata and fusarium moniforme.

Description

technical field [0001] The present invention relates to a strain of streptomyces. Background technique [0002] Poplar gray spot is one of the diseases that harm poplar leaves caused by the fungus Sporocadus populinus (Bres.) Orsenigo, Rodondi & B. Sutton=Coryneum populinum Bres. It has a large area of ​​occurrence and serious damage. Occurs on the leaves and treetops of saplings and seedlings, causing leaves to fall off early, cadres canker, young shoots withered, and growth is reduced. In severe cases, it can cause plant death, reduce the survival rate of transplanting, and threaten poplar seedling cultivation and afforestation. very big. [0003] Plant diseases are one of the important factors that threaten agricultural production. Currently, the use of chemical pesticides is the main method to control plant diseases. However, the long-term use of chemical pesticides not only pollutes the environment and destroys the ecological balance, but also the residues are harmful ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/465
Inventor 刘雪英马玲刘雪峰
Owner NORTHEAST FORESTRY UNIVERSITY
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